蠕虫

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    现刊
    Detection of piggyBac-mediated Transposition by Splinkerette PCR in Transgenic Lines of Strongyloides ratti
    采用Splinkerette PCR检测类圆线虫转基因株系中的piggyBac介导转位
    作者:Hongguang Shao and James B. Lok日期:01/05/2014,浏览量:13516,Q&A: 0
    [Abstract] Splinkerette PCR (spPCR) is a newly developed and efficient method to ascertain and characterize genomic insertion sites of transgenes. The method described in this protocol was successfully applied to confirm piggyBac transposon-mediated integration of transgenes into chromosomes of the parasitic nematode Strongyloides ratti. ...