分子生物学

分类

    现刊
    A Modified Fluctuation Assay with a CAN1 Reporter in Yeast
    酵母中CAN1报告基因的改良波动测定
    作者:Pengyao Jiang, Anja R. Ollodart and Maitreya J. Dunham日期:06/05/2022,浏览量:1012,Q&A: 0
    [Abstract]

    Understanding the generation of mutations is fundamental to understanding evolution and genetic disease; however, the rarity of such events makes experimentally identifying them difficult. Mutation accumulation (MA) methods have been widely used. MA lines require serial bottlenecks to fix de novo mutations, followed by whole-genome sequencing.

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    Determination of Antibacterial Activity of Film Coatings against Four Clinically Relevant Bacterial Strains
    薄膜敷层对4种与临床相关菌株的抑菌活性测定
    [Abstract]

    Antibacterial coatings have currently gained great importance in biomedical technology investigations. Because of the spatial arrangement of the film coatings, evaluation of antibacterial activity presents a new challenge regarding traditional bacterial counting methods. In this protocol, four clinically relevant pathogens, Salmonella typhimurium,

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    Isolation and Quantification of Extracellular DNA from Biofluids
    生物流体中细胞外DNA的分离和定量
    作者:Ľubica Janovičová, Barbora Konečná, Barbora Vlková and Peter Celec日期:08/20/2020,浏览量:3029,Q&A: 0
    [Abstract] Extracellular DNA is studied as a diagnostic biomarker, but also as a factor involved in the pathophysiology of several diseases due to its pro-inflammatory properties. Extracellular DNA can be extracted from plasma, urine, saliva or other biofluids using standard DNA isolation procedures and specialized commercial kits. Sample preparation for ...
    Transcervical Mouse Infections with Chlamydia trachomatis and Determination of Bacterial Burden
    小鼠宫颈沙眼衣原体感染及细菌负荷测定
    作者:Karthika Rajeeve and Rajeeve Sivadasan日期:02/05/2020,浏览量:4242,Q&A: 0
    [Abstract] Chlamydia trachomatis is an obligate human pathogen. It infects the genital tract of humans ascending into the fallopian tube, exacerbated by chronic pelvic pain, pelvic inflammatory disease, and fallopian tube scaring resulting in infertility and other malignancies. The major hurdle in controlling chlamydial spread is that the ...
    DNase I Chromatin Accessibility Analysis
    DNaseⅠ染色质可及性分析
    作者:Brook S. Nepon-Sixt and Mark G. Alexandrow日期:12/05/2019,浏览量:4050,Q&A: 0
    [Abstract] Chromatin consists of compacted DNA in complex with proteins and contributes to the organization of DNA and its stability. Furthermore, chromatin plays key roles in regulating cellular processes such as DNA replication, transcription, DNA repair, and mitosis. Chromatin assumes more compact (inaccessible) or decondensed (accessible) conformations ...
    Quantification of HIV-2 DNA in Whole Blood
    全血HIV-2 DNA的定量分析
    作者:Zsófia Szojka, Sara Karlson, Marianne Jansson and Patrik Medstrand日期:10/20/2019,浏览量:4284,Q&A: 0
    [Abstract] Time to AIDS infection is longer with HIV-2, compared to HIV-1, but without antiretroviral therapy both infections will cause AIDS-related mortality. In HIV-2 infection, monitoring of antiretroviral treatment (ART) efficacy is challenging since a large proportion of HIV-2-infected individuals displays low or undetectable plasma RNA levels. Hence, ...
    Analysis of Early Phase HIV-1 Replication and Integration Events by Using Real-time PCR
    利用实时PCR对早期HIV-1复制和整合过程进行检测
    作者:Graf Laura, Steven P. Moran, Sally Ro, Carlos M.C. de Noronha and Binshan Shi日期:02/20/2019,浏览量:4630,Q&A: 0
    [Abstract] Upon entry into a host cell, the HIV-1 virus undergoes a series of critical early replication events including reverse transcription, nuclear import, and integration of its cDNA into the host genome. Molecular assays used to detect and analyze changes in HIV-1 early phase replication events are valuable tools in developing potential antiretroviral ...
    Virus Binding and Internalization Assay for Adeno-associated Virus
    腺相关病毒的病毒结合和内化测定实验
    作者:Garrett E. Berry and Longping V. Tse日期:01/20/2017,浏览量:11106,Q&A: 0
    [Abstract] The binding and internalization of adeno-associated virus (AAV) is an important determinant of viral infectivity and tropism. The ability to dissect these two tightly connected cellular processes would allow better understanding and provide insight on virus entry and trafficking. In the following protocol, we describe a quantitative PCR (qPCR) ...
    Estimation of the Chromosomal Copy Number in Synechococcus elongatus PCC 7942
    聚球藻PCC 7942中染色体拷贝数的估测
    作者:Satoru Watanabe and Hirofumi Yoshikawa日期:07/05/2016,浏览量:7489,Q&A: 0
    [Abstract] Cyanobacteria are prokaryotic organisms that perform oxygenic photosynthesis. Freshwater cyanobacteria, such as Synechococcus elongatus PCC 7942 and Synechocystis sp. PCC 6803, are model organisms for the study of photosynthesis, gene regulation, and biotechnological applications because they are easy to manipulate ...
    Determination of Mitochondrial DNA Upon Drug Treatment
    药物处理后的线粒体DNA测定
    作者:Michel Perron and Joy Y. Feng日期:01/20/2015,浏览量:7820,Q&A: 0
    [Abstract] Drug-induced mitochondrial injury can be caused by many different mechanisms including inhibition of mitochondrial DNA replication, transcription, translation, and altered protein function. Determination of the level of mitochondrial DNA relative to the nuclear DNA levels provides important information on potential mitochondrial toxicity.