病毒

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    现刊
    Selection of Genetically Modified Bacteriophages Using the CRISPR-Cas System
    利用CRISPR-Cas系统筛选基因组编辑的噬菌体
    作者:Miriam Manor and Udi Qimron日期:08/05/2017,浏览量:9673,Q&A: 0
    [Abstract] We present a CRISPR-Cas based technique for deleting genes from the T7 bacteriophage genome. A DNA fragment encoding homologous arms to the target gene to be deleted is first cloned into a plasmid. The T7 phage is then propagated in Escherichia coli harboring this plasmid. During this propagation, some phage genomes undergo homologous ...