癌症生物学

Advertisement

分类

    现刊
    Triacylglycerol Measurement in HeLa Cells
    HeLa细胞中三酰甘油的测定
    作者:Ximing Du and Hongyuan Yang日期:12/20/2020,浏览量:2171,Q&A: 0
    [Abstract]

    Lipid droplets store triacylglycerols (triglycerides) and sterol esters to regulate lipid and energy homeostasis. Triacylglycerol measurement is often performed during the investigation of lipid droplet formation and growth. This protocol describes a reliable method using a fluorometric lipid quantification kit to measure triacylglycerols

    ...
    Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader
    使用荧光读板仪通过钙指示剂Fura-2 AM 测定细胞内钙浓度
    作者:Magdiel Martínez, Namyr A. Martínez and Walter I. Silva日期:07/20/2017,浏览量:29438,Q&A: 1
    [Abstract] Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to agonists/antagonists, among others (Burgos et al., 2007; Martínez et al., 2016). Changes in intracellular calcium concentration can be measured ...
    Metabolic Assays for Detection of Neutral Fat Stores
    代谢试验检测中性脂肪储存
    作者:Jan M. Baumann, Leila Kokabee, Xianhiu Wang, Yan Sun, Jason Wong and Douglas S. Conklin日期:06/20/2015,浏览量:13823,Q&A: 1
    [Abstract] Lipid droplets (LDs) are ubiquitous intracellular structures whose formation, growth, and maintenance are highly regulated (Wang et al., 2013; Ranall et al., 2011; Goodman, 2009). Lipid metabolism and droplet dynamics are of considerable interest to agriculture, biofuel production, viral pathology, nutrition, and cancer biology ...
    Bone Resorption Assay
    骨吸收分析
    作者:Carina Scholtysek, Gerhard Krönke and Georg Schett日期:07/20/2014,浏览量:15308,Q&A: 0
    [Abstract] The Bone resorption assay provides an easy to use protocol for quantitatively measuring in vitro osteoclast-mediated bone resorption. Osteoclasts can be seeded onto the bone slices and formation of resorption pits can be quantified via toluidinblue staining (Scholtysek et al., 2013).
    Lysosomal Stability Assay
    溶酶体稳定性分析
    作者:Nikolaj H. T. Petersen, Thomas Kirkegaard and Marja Jäättelä日期:06/20/2014,浏览量:15823,Q&A: 0
    [Abstract] This assay makes use of the dye Acridine Orange (AO) to determine the stability of lysosomes in living cells upon exposure to a confocal microscope laser.

    AO is a lipophilic amine that readily diffuses into cells (Figure 1). Inside the cell it enters the acidic lysosomal compartment where it is protonated and sequestered, shifting its ...
    Homologous Recombination Assay
    同源重组分析
    作者:Yvan Canitrot and Didier Trouche日期:09/20/2013,浏览量:17094,Q&A: 0
    [Abstract] Repair of double strand break by homologous recombination was examined using U2OS cells or RG37 cells harbouring specific substrate developed by Puget et al. (2005) and Dumay et al. (2006), respectively, to measure the repair of DNA double strand breaks by homologous recombination. The substrate is composed of two inactive copies ...