植物

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    现刊
    Histone modification ChIP-seq on Arabidopsis thaliana Plantlets
    拟南芥植株上的组蛋白修饰ChIP-seq
    作者:Juan S. Ramirez-Prado, David Latrasse and Moussa Benhamed日期:11/05/2021,浏览量:1897,Q&A: 1
    [Abstract]

    Characterizing the molecular mechanisms regulating gene expression is crucial for understanding the regulatory processes underlying physiological responses to environmental and developmental signals in eukaryotes. The covalent modification of histones contributes to the compaction levels of chromatin, as well as the recruitment of the

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    Determination of Luciferase Activity in Arabidopsis seedling
    拟南芥幼苗中荧光素酶活性的测定
    作者:Mohan TC, Gabriel Castrillo and Antonio Leyva日期:06/20/2014,浏览量:11635,Q&A: 0
    [Abstract] There are different direct and indirect methods available to study gene expression in plant systems. In this protocol we describe a modified expression assay using transgenic plants expressing the luciferase gene under the control of phosphate transporter PHT1;1 promoter. This assay was originally optimized for analyzing the repression of PHT1;1 ...
    DNA Damage Sensitivity Assays with Arabidopsis Seedlings
    拟南芥幼苗的DNA损伤敏感性实验
    作者:Marisa Rosa and Ortrun Mittelsten Scheid日期:04/05/2014,浏览量:9852,Q&A: 0
    [Abstract] We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a radiomimetic drug, (3) mitomycin C, a DNA intrastrand cross-linker, (4) hydroxyurea, an inhibitor of DNA ...
    Measuring Homologous Recombination Frequency in Arabidopsis Seedlings
    拟南芥幼苗中同源重组频率的测定
    作者:Marisa Rosa and Ortrun Mittelsten Scheid日期:04/05/2014,浏览量:11534,Q&A: 0
    [Abstract] Somatic homologous recombination (SHR) is a major pathway of DNA double-strand break (DSB) repair, in which intact homologous regions are used as a template for the removal of lesions. Its frequency in plants is generally low, as most DSB are removed by non-homologous mechanisms in higher eukaryotes. Nevertheless, SHR frequency has been shown to ...
    Micrococcal Nuclease (MNase) Assay of Arabidopsis thaliana Nuclei
    微球菌核酸酶(Mnase)试验分析拟南芥染色质结构
    作者:Laia Armengot and Jordi Moreno-Romero日期:04/05/2013,浏览量:22281,Q&A: 4
    [Abstract] Micrococcal nuclease (MNase) is able to produce double-strand breaks within nucleosome linker regions. The efficiency of MNase digestion depends on the degree of chromatin compaction, being more easily digested the regions of less compacted chromatin. The MNase protocol described here can be used to asses changes in the chromatin structure of ...