节肢动物

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    现刊
    pNP Transgenic RNAi System Manual in Drosophila
    果蝇中pNP RNAi 转基因系统手册
    作者:Fang Wang, Huan-Huan Qiao, Rong-Gang Xu, Jin Sun, Ruibao Zhu, Decai Mao and Jian-Quan Ni 日期:02/05/2019,浏览量:5429,Q&A: 0
    [Abstract] Much of our knowledge about the mechanisms underlying biological processes relies on genetic approaches, whereby gene activity is reduced and the phenotypic consequences of perturbation are analyzed in detail. For functional genomic studies, a specific, systematic, and cost-effective manner is critical. Transgenic RNAi system is the top priority ...
    Flip-flop Mediated Conditional Gene Inactivation in Drosophila
    果蝇中Flip-flop介导的条件性基因失活
    作者:Sathiya N. Manivannan, Priyanka Pandey and Sonal Nagarkar-Jaiswal日期:02/05/2019,浏览量:5594,Q&A: 0
    [Abstract] Mosaic analysis in Drosophila, an important tool to assess cellular phenotypes of mutants in an otherwise heterozygous background, relies on mitosis. Hence, it cannot be used to inactivate gene function in mitotically inactive, terminally differentiated cells such as neurons. To address this issue, we developed “Flip-flop”, a novel, ...
    flySAM Transgenic CRISPRa System Manual
    基于flySAM的CRISPRa转基因系统操作手册
    作者:Yu Jia, Da Shen, Xia Wang, Jin Sun, Ping Peng, Rong-Gang Xu, Bowen Xu and Jian-Quan Ni 日期:01/20/2019,浏览量:6620,Q&A: 0
    [Abstract] Powerful and general methods that can enhance gene expression are useful to systematically study gene function. To date, compared with the methods in generating loss-of-function mutants, methods to achieve gain-of-function are limited. The entire field in Drosophila has relied heavily on the Gal4/UAS:cDNA overexpression system developed ...
    Validating Candidate Congenital Heart Disease Genes in Drosophila
    果蝇中候选先天性心脏病基因的验证
    作者:Jun-yi Zhu, Yulong Fu, Adam Richman and Zhe Han日期:06/20/2017,浏览量:7612,Q&A: 0
    [Abstract] Genomic sequencing efforts can implicate large numbers of genes and de novo mutations as potential disease risk factors. A high throughput in vivo model system to validate candidate gene association with pathology is therefore useful. We present such a system employing Drosophila to validate candidate congenital heart ...
    FLP/FRT Induction of Mitotic Recombination in Drosophila Germline
    果蝇种系中有丝分裂重组的FLP/FRT诱导
    作者:Pedro Prudêncio and Leonardo G. Guilgur日期:05/05/2015,浏览量:27150,Q&A: 0
    [Abstract] The FLP/FRT system is a site-directed recombination technology based on the targeting of a recombination enzyme (flipase - FLP) to specific DNA regions designated as flipase recognition target (FRT) sites. Initially identified in Saccharomyces cerevisiae, the yeast FLP-enzyme and its FRT recombination targets were successfully transferred ...