原核生物

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    Nitrite Reduction Assay for Whole Pseudomonas Cells
    全假单胞菌细胞的亚硝酸盐还原试验
    作者:Michael P. Thorgersen and Michael W. Adams日期:05/20/2016,浏览量:10746,Q&A: 1
    [Abstract] The second step of the dissimilatory denitrification pathway in which nitrite (NO2-) is converted to nitric oxide (NO) is catalyzed by the enzyme nitrite reductase. Two distinct enzymes are found in nature that catalyze this reaction, and they contain different metal sites, either iron (Fe), in the form of heme, or copper ...
    Identification and Characterization of Bacterial Chemoreceptors Using Quantitative Capillary and Gradient Plate Chemotaxis Assays
    使用量化毛细管和梯度平板趋化性试验法进行细菌化学受体的识别和特性描述
    作者:Jose Antonio Reyes-Darias, Vanina García, Miriam Rico-Jiménez, Andrés Corral-Lugo and Tino Krell日期:04/20/2016,浏览量:10558,Q&A: 0
    [Abstract] Bacterial chemotaxis is a motility-based response that biases cell movement toward beneficial molecules, called attractants, and away from harmful molecules, also known as repellents. Since the species of the genus Pseudomonas are characterized by a metabolic versatility, these bacteria have developed chemotactic behaviors towards a wide range of ...
    Quantification of Respiratory Activity in Biofilms
    生物膜呼吸活性的量化
    作者:Cláudia N. H. Marques and Scott A. Craver日期:09/20/2015,浏览量:9440,Q&A: 1
    [Abstract] Bacteria live mostly as biofilms, not as planktonic cell populations. Bacterial cells living as biofilms are known to be in different physiological status. Persister cells are one of such physiological conditions and they are recognized as to be a stochastically produced sub-population of non-growing bacterial cells. The following protocol ...
    Isolation of Persister Cells from Biofilm and Planktonic Populations of Pseudomonas aeruginosa
    从绿脓杆菌生物膜和浮游种群中分离持留细胞
    作者:Cláudia N. H. Marques日期:09/20/2015,浏览量:11371,Q&A: 0
    [Abstract] Persister cells are a stochastically produced sub-population of non-growing bacterial cells. Recently these cells have been more widely studied due to the recognition that they are tolerant to antimicrobials and thus, play a major role in the resilience of bacterial populations to antimicrobials, particularly in chronic biofilm infections. The ...
    Cell Fractionation of Pseudomonas aeruginosa
    绿脓杆菌的细胞分级分离
    作者:Esteban Paredes-Osses and Kim R. Hardie日期:10/05/2013,浏览量:11921,Q&A: 1
    [Abstract] Pseudomonas aeruginosa is a Gram negative bacterium. Separating the cell envelope compartments enables proteins to be localized to confirm where in the cell they function. Cell fractionation can also provide a first step in a protein purification strategy (Williams et al., 1998). This protocol has been designed to obtain the ...