病毒

分类

    现刊
    Generation and Implementation of Reporter BHK-21 Cells for Live Imaging of Flavivirus Infection
    黄病毒感染活体显像报告细胞BHK-21的制备与实施
    作者:Jorge L. Arias-Arias and Rodrigo Mora-Rodríguez日期:03/05/2021,浏览量:1971,Q&A: 0
    [Abstract]

    The genus Flavivirus within the family Flaviviridae includes many viral species of medical importance, such as yellow fever virus (YFV), Zika virus (ZIKV), and dengue virus (DENV), among others. Presently, the identification of flavivirus-infected cells is based on either the immunolabeling of viral proteins, the application of recombinant

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    Production and Purification of Dengue Virus-like Particles from COS-1 Cells
    从COS-1细胞中制备和纯化登革热病毒样颗粒
    作者:Jedhan Ucat Galula, Gwong-Jen J. Chang and Day-Yu Chao日期:06/20/2019,浏览量:6899,Q&A: 0
    [Abstract] Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires abundant, and high-purity production methods. ...
    Virucidal and Neutralizing Activity Tests for Antiviral Substances and Antibodies
    抗病毒物质和抗体的杀灭病毒活性及中和活性检测
    作者:Chie Aoki-Utsubo, Ming Chen and Hak Hotta日期:05/20/2018,浏览量:10880,Q&A: 0
    [Abstract] In a narrow definition, virucidal activity represents the activity by which to interact with and physically disrupt viral particles. In a broad definition, it includes the activity by which to functionally inhibit (neutralize) viral infectivity without apparent morphological alterations of the viral particles. The viral infectivity can be measured ...
    Time-of-addition and Temperature-shift Assays to Determine Particular Step(s) in the Viral Life Cycle that is Blocked by Antiviral Substance(s)
    改变药物添加时间及变温分析实验确定抗病毒物质所作用的病毒生命周期中的步骤
    作者:Chie Aoki-Utsubo, Ming Chen and Hak Hotta日期:05/05/2018,浏览量:7143,Q&A: 0
    [Abstract] Viruses infect their host cells to produce progeny virus particles through the sequential steps of the viral life cycle, such as viral attachment, entry, penetration and post-entry events. This protocol describes time-of-addition and temperature-shift assays that are employed to explore which step(s) in the viral life cycle is blocked by an ...
    In vitro Assays for Measuring Endothelial Permeability by Transwells and Electrical Impedance Systems
    利用Transwell试验和电阻抗系统体外测量内皮通透性
    作者:Hong-Ru Chen and Trai-Ming Yeh日期:05/05/2017,浏览量:23245,Q&A: 2
    [Abstract] Vascular leakage is an important feature in several diseases, such as septic shock, viral hemorrhagic fever, cancer metastasis and ischemia-reperfusion injuries. Thus establishing assays for measuring endothelial permeability will provide insight into the establishment or progression of such diseases. Here, we provide transwell permeability assay ...
    Assay to Evaluate Vascular Permeability Induction in Mice
    小鼠血管通透性诱导的分析评估
    [Abstract] Dengue virus infection usually courses as a benign self-limited fever, called dengue fever. However, on occasions it can progress to a life-threatening complication known as severe dengue (SD). A hallmark of SD is a sharp increase in vascular permeability. Secondary infections are considered a risk factor to develop SD, presumably through a ...