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Measurement of Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in Culture Cells for Assessment of the Energy Metabolism
测定培养细胞中的消氧率(OCR)和细胞外酸化率(ECAR)以评估能量代谢
作者:Birte Plitzko and Sandra Loesgen日期:05/20/2018,浏览量:21016,Q&A: 0
Mammalian cells generate ATP by mitochondrial (oxidative phosphorylation) and non-mitochondrial (glycolysis) metabolism. Cancer cells are known to reprogram their metabolism using different ...
BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
显微镜观察BODIPY 493/503 染色中性脂滴以及用流式细胞仪量化
作者:Bo Qiu and M. Celeste Simon日期:09/05/2016,浏览量:29118,Q&A: 0
Lipid droplets (LDs) are ubiquitous, dynamic organelles and function as a storage depot for neutral lipids, including triglycerides and cholesterol esters (Walther and Farese, 2012). The movement of ...
Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
利用磺酰罗丹明B(SRB)法检测细胞的增殖
作者:Esteban A. Orellana and Andrea L. Kasinski日期:11/05/2016,浏览量:29815,Q&A: 0
The SRB assay has been used since its development in 1990 (Skehan et al., 1990) to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies (Vichai ...
Superoxide Dismutase (SOD) and Catalase (CAT) Activity Assay Protocols for Caenorhabditis elegans
秀丽隐杆线虫中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性测定实验方案
作者:Jing Zhang, Rui Chen, Zhenyang Yu and Lili Xue日期:08/20/2017,浏览量:16591,Q&A: 0
Assays for superoxide dismutase (SOD) and catalase (CAT) activities are widely employed to indicate antioxidant responses underlying the toxic effects of test chemicals. Yet, earlier studies mainly ...
Cell Synchronization by Double Thymidine Block
用胸腺嘧啶核苷双阻断法同步细胞
作者:Guo Chen and Xingming Deng日期:09/05/2018,浏览量:12016,Q&A: 1
Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine ...
Analysis of Protein Stability by the Cycloheximide Chase Assay
放线菌酮追踪检测分析蛋白质的稳定性
Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly ...
Microbial Mutagenicity Assay: Ames Test
微生物诱变分析:Ames试验
作者:Urvashi Vijay, Sonal Gupta, Priyanka Mathur, Prashanth Suravajhala and Pradeep Bhatnagar日期:03/20/2018,浏览量:12825,Q&A: 2
The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to ...
Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader
使用荧光读板仪通过钙指示剂Fura-2 AM 测定细胞内钙浓度
作者:Magdiel Martínez, Namyr A. Martínez and Walter I. Silva日期:07/20/2017,浏览量:14066,Q&A: 0
Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to ...
Isolation of PBMCs Using Vacutainer® Cellular Preparation Tubes (CPTTM)
使用Vacutainer®细胞制备管(CPTTM)分离外周血单核细胞
作者:Alaina Puleo, Chantia Carroll, Holden Maecker and Rohit Gupta日期:01/20/2017,浏览量:18321,Q&A: 0
Peripheral blood mononuclear cell (PBMC) isolation is commonly done via density gradient centrifugation over Ficoll-Hypaque, a labor-intensive procedure that requires skilled technicians and can ...
Soft Agar Colony Formation Assay as a Hallmark of Carcinogenesis
软琼脂集落形成实验测定作为致癌性标志的集落形成
作者:Feng Du, Xiaodi Zhao and Daiming Fan日期:06/20/2017,浏览量:13418,Q&A: 0
Soft agar colony formation assay is established to estimate the anchorage-independent growth ability of cells. In this assay, a bottom layer of agar with complete media is poured and solidified ...