热门实验
Computational Analysis and Phylogenetic Clustering of SARS-CoV-2 Genomes
SARS-CoV-2基因组计算分析及系统进化聚类分析
作者:Bani Jolly and Vinod Scaria日期:04/20/2021,浏览量:1353,Q&A: 0

COVID-19, the disease caused by the novel SARS-CoV-2 coronavirus, originated as an isolated outbreak in the Hubei province of China but soon created a global pandemic and is now a major threat to

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Quantitative Characterization of the Amount and Length of (1,3)-β-D-glucan for Functional and Mechanistic Analysis of Fungal (1,3)-β-D-glucan Synthase
通过(1,3)-β-d-葡聚糖量和长度的定量表征对真菌(1,3)-β-d-葡聚糖合酶进行功能和机理分析
作者:Abhishek Chhetri, Anna Loksztejn and Kenichi Yokoyama日期:04/20/2021,浏览量:993,Q&A: 0

(1,3)-β-d-Glucan synthase (GS) is an essential enzyme for fungal cell wall biosynthesis that catalyzes the synthesis of (1,3)-β-d-glucan, a major and vital component of the cell wall. GS is

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Screening for Lysogen Activity in Therapeutically Relevant Bacteriophages
筛选与治疗相关的噬菌体的溶原活性
作者:Fernando L. Gordillo Altamirano and Jeremy J. Barr日期:04/20/2021,浏览量:1381,Q&A: 1

Lysogenic phages can integrate into their bacterial host’s genome, potentially transferring any genetic information they possess including virulence or resistance genes, and are therefore

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A Sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA while Preserving Cellular DNA
一种在保留细胞DNA的同时定量检测乙型肝炎病毒共价闭合环状(ccc)DNA的灵敏、特异PCR方法
作者:Benno Zehnder, Stephan Urban and Thomas Tu日期:04/20/2021,浏览量:867,Q&A: 0

Hepatitis B virus (HBV) is the major cause of liver diseases and liver cancer worldwide. After infecting hepatocytes, the virus establishes a stable episome (covalently closed circular DNA, or

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Optimized Recombinant Production of Secreted Proteins Using Human Embryonic Kidney (HEK293) Cells Grown in Suspension
利用悬浮培养的人胚胎肾(HEK293)细胞优化分泌蛋白的重组生产

Recombinant proteins are an essential milestone for a plethora of different applications ranging from pharmaceutical to clinical, and mammalian cell lines are among the currently preferred systems to

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Liposomal Clodronate-mediated Macrophage Depletion in the Zebrafish Model
在斑马鱼模型中脂质体氯膦酸盐介导的巨噬细胞耗竭
作者:Linlin Yang, Alison M. Rojas and Celia E. Shiau日期:03/20/2021,浏览量:1143,Q&A: 0

The ability to conduct in vivo macrophage-specific depletion remains an effective means to uncover functions of macrophages in a wide range of physiological contexts. Compared to the murine model,

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Multilayered Fabrication Assembly Technique to Engineer a Corneal Stromal Equivalent
用多层制造组装技术制做角膜基质等效物
作者:Julia Fernández-Pérez, Peter W. Madden and Mark Ahearne日期:03/20/2021,浏览量:1028,Q&A: 0

Tissue engineering has emerged as a strategy to combat the donor shortage of human corneas for transplantation. Synthetic corneal substitutes are currently unable to support the normal phenotype of

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Intestinal Co-culture System to Study TGR5 Agonism and Gut Restriction
利用肠道共培养系统研究TGR5激动剂和肠道限制性
作者:Snehal N. Chaudhari and A. Sloan Devlin日期:03/20/2021,浏览量:1157,Q&A: 0

The activation of the Takeda G-protein receptor 5 (TGR5, also known as the G protein-coupled bile acid receptor 1, GPBAR1) in enteroendocrine L-cells results in secretion of the anti-diabetic hormone

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Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells
用RUSH蛋白分选法追踪HeLa细胞分泌通路中的活细胞囊泡
作者:Mehrshad Pakdel, Natalia Pacheco-Fernandez and Julia von Blume日期:03/20/2021,浏览量:1075,Q&A: 0

More than 30% of the total amount of proteins synthesized in mammalian cells follow the secretory pathway in order to mature and be properly sorted to their final destinations. Among several

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Colorimetric RT-LAMP and LAMP-sequencing for Detecting SARS-CoV-2 RNA in Clinical Samples
比色RT-LAMP和LAMP测序检测临床样本中SARS-CoV-2 RNA
作者:Konrad Herbst, Matthias Meurer, Daniel Kirrmaier, Simon Anders, Michael Knop and Viet Loan Dao Thi日期:03/20/2021,浏览量:1204,Q&A: 0

During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to rapidly test large numbers of people are needed. As a faster and less resource-demanding alternative to detect

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Murine Monocyte and Macrophage Culture
小鼠单核细胞和巨噬细胞培养
作者:Simone M. Haag and Aditya Murthy日期:03/20/2021,浏览量:2273,Q&A: 0

Myeloid progenitors in the bone marrow generate monocytes, macrophages, granulocytes and most dendritic cells. Even though these innate immune cells are part of the same lineage, each cell type plays

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Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids
受荧光标记和易于固化质粒影响的假单胞菌快速基因组工程
作者:Daniel C. Volke, Nicolas T. Wirth and Pablo I. Nikel日期:02/20/2021,浏览量:2213,Q&A: 0

Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for

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Expression and Purification of Yeast-derived GPCR, Gα and Gβγ Subunits for Structural and Dynamic Studies
重组酵母的GPCR、Gα和Gβγ亚基的表达和纯化,用于结构和动力学研究
作者:Wenjie Zhao, Xudong Wang and Libin Ye日期:02/20/2021,浏览量:1828,Q&A: 0

In the last several years, as evidence of a surged number of GPCR-G complex structures, the expressions of GPCRs and G proteins for structural biology have achieved tremendous successes, mostly in

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Evaluation of the Sequence Variability within the PCR Primer/Probe Target Regions of the SARS-CoV-2 Genome
SARS-CoV-2基因组PCR引物/探针靶区序列变异性评价
作者:Kashif Aziz Khan and Peter Cheung日期:12/20/2020,浏览量:1612,Q&A: 0
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; initially named 2019-nCoV) is responsible for the recent coronavirus disease (COVID-19) pandemic, and polymerase chain reaction (PCR) is ...
Native Co-immunoprecipitation Assay to Identify Interacting Partners of Chromatin-associated Proteins in Mammalian Cells
天然共免疫沉淀法鉴定哺乳动物细胞中染色质相关蛋白的“伙伴蛋白质”
作者:Afzal Husain, Nasim A. Begum, Maki Kobayashi and Tasuku Honjo日期:12/05/2020,浏览量:1949,Q&A: 0

Protein-protein interactions play key roles in nuclear processes including transcription, replication, DNA damage repair, and recombination. Co-immunoprecipitation (Co-IP) followed by western blot or

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Agrobacterium-mediated Transformation of Sweet Basil (Ocimum basilicum)
农杆菌介导的罗勒(Ocimum basilicum)转化
作者:Natasha Navet and Miaoying Tian日期:11/20/2020,浏览量:1886,Q&A: 1

Sweet basil (Ocimum basilicum) is a popular herb with high economic value and is currently threatened by a severe oomycete disease. An efficient transformation method is a prerequisite for gene

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Colorimetric RT-LAMP Methods to Detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
严重急性呼吸综合征冠状病毒2(SARS-CoV-2)的RT-LAMP比色法检测
作者:Gun-Soo Park, Seung-Hwa Baek, Keunbon Ku, Seong Jun Kim, Seung Il Kim, Bum-Tae Kim and Jin-Soo Maeng日期:11/05/2020,浏览量:1947,Q&A: 0
Standard diagnostic methods of Coronavirus Disease 2019 (COVID-19) rely on RT-qPCR technique which have limited point-of-care test (POCT) potential due to necessity of dedicated equipment and ...
Dual sgRNA-based Targeted Deletion of Large Genomic Regions and Isolation of Heritable Cas9-free Mutants in Arabidopsis
基于双sgRNA的拟南芥大基因组区域靶向清除及可遗传的非Cas9突变体的分离
作者:Yu Jin and Sebastian Marquardt日期:10/20/2020,浏览量:2225,Q&A: 0
CRISPR/Cas9 system directed by a gene-specific single guide RNA (sgRNA) is an effective tool for genome editing such as deletions of few bases in coding genes. However, targeted deletion of ...
Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides
植物细胞壁多糖共聚焦和超分辨成像的多靶点免疫组织化学研究
作者:Kalina T. Haas, Methieu Rivière, Raymond Wightman and Alexis Peaucelle日期:10/05/2020,浏览量:2285,Q&A: 0
The plant cell wall (PCW) is a pecto-cellulosic extracellular matrix that envelopes the plant cell. By integrating extra-and intra-cellular cues, PCW mediates a plethora of essential physiological ...
A Workflow for Ultra-rapid Analysis of Histone Post-translational Modifications with Direct-injection Mass Spectrometry
使用直接进样质谱对组蛋白翻译后修饰进行超快速分析的工作流程
作者:Natarajan V. Bhanu, Simone Sidoli and Benjamin A Garcia日期:09/20/2020,浏览量:2357,Q&A: 0
Chromatin modifications, like histone post translational modifications (PTMs), are critical for tuning gene expression and many other aspects of cell phenotype. Liquid chromatography coupled to mass ...
Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells
细胞系和原代免疫细胞中的低水平活性氧的流式细胞仪检测
作者:Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd日期:09/05/2020,浏览量:2720,Q&A: 0
Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst ...
In vivo Blood-brain Barrier Permeability Assays Using Clostridium perfringens Epsilon Toxin
应用产气荚膜梭菌ε毒素测定体内血脑屏障通透性
作者:Michael R. Mazzucco, Timothy Vartanian and Jennifer R. Linden日期:08/05/2020,浏览量:2316,Q&A: 0
In order for the brain to function properly, a carefully orchestrated homeostasis must be maintained. To help regulate this delicate balance, the brain has developed a highly selective blood-brain ...
Measuring Intracellular Vesicle Density and Dispersion Using Fluorescence Microscopy and ImageJ/FIJI
使用荧光显微镜和ImageJ / FIJI测量细胞内囊泡的密度和分散度
Cell signalling, cell secretion, and plasma membrane repair are processes that critically rely on intracellular vesicles, important components of the endocytic and secretory pathways. More ...
Preparation of Drosophila Polytene Chromosomes, Followed by Immunofluorescence Analysis of Chromatin Structure by Multi-fluorescence Correlations
制备果蝇多线染色体并通过多重荧光关联对染色质结构进行免疫荧光分析
作者:Terra M. Kuhn, Shawn C. Little and Maya Capelson日期:07/05/2020,浏览量:4337,Q&A: 0
Drosophila larval salivary gland polytene chromosome squashes have been used for decades to analyze genome-wide protein-binding patterns, transcriptional activation processes, and changes in ...
Mouse Adipose Tissue Protein Extraction
小鼠脂肪组织蛋白提取
作者:Yu A. An and Philipp E. Scherer日期:06/05/2020,浏览量:4070,Q&A: 0
As obesity becomes a global epidemic, the metabolism research field is increasingly focusing on studying the physiological and pathological roles of adipose tissues (AT). However, extracting proteins ...
Confocal and Super-resolution Imaging of RNA in Live Bacteria Using a Fluorogenic Silicon Rhodamine-binding Aptamer
含氟硅-罗丹明适配体用于活性菌中RNA的激光共聚焦超分辨率成像
作者:Regina Wirth, Peng Gao, G. Ulrich Nienhaus, Murat Sunbul and Andres Jäschke日期:05/05/2020,浏览量:4087,Q&A: 0
Genetically encoded light-up RNA aptamers have been shown to be promising tools for the visualization of RNAs in living cells, helping us to advance our understanding of the broad and complex life of ...
Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA
通过合成共价闭合环状(3Cs)DNA构建无偏和专用CRISPR / Cas gRNA文库
作者:Martin Wegner, Koraljka Husnjak and Manuel Kaulich日期:01/05/2020,浏览量:4445,Q&A: 0
Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus ...
Tryptophan Fluorescence Quenching Assays for Measuring Protein-ligand Binding Affinities: Principles and a Practical Guide
用于检测蛋白配体结合亲和力的色氨酸荧光猝灭试验:原理和操作指南
作者:Anthony Yammine, Jinlong Gao and Ann H. Kwan日期:06/05/2019,浏览量:12391,Q&A: 0
Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a ...
Analysis of Indole-3-acetic Acid (IAA) Production in Klebsiella by LC-MS/MS and the Salkowski Method
利用液相色谱-串联质谱法和萨尔科夫斯基氏法分析克雷伯菌中吲哚乙酸的产生
作者:Shraddha Gang, Sheetal Sharma, Meenu Saraf, Martin Buck and Jörg Schumacher日期:05/05/2019,浏览量:15094,Q&A: 1
Many rhizobacteria isolated from plant rhizosphere produce various phytohormones in the form of secondary metabolites, the most common of which is Indole-3-acetic acid (IAA). Here, we detail ...
Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells
利用单分子荧光原位杂交进行贴壁动物细胞中RNA的检测
作者:Gal Haimovich and Jeffrey E. Gerst日期:11/05/2018,浏览量:23242,Q&A: 1
Transcription and RNA decay play critical roles in the process of gene expression and the ability to accurately measure cellular mRNA levels is essential for understanding this regulation. Here, we ...
mRNA Stability Assay Using Transcription Inhibition by Actinomycin D in Mouse Pluripotent Stem Cells
利用放线菌素D抑制转录检测小鼠多能干细胞中mRNA的稳定性
作者:Madara Ratnadiwakara and Minna-Liisa Änkö日期:11/05/2018,浏览量:20659,Q&A: 2
Gene expression is regulated through multiple steps at both transcriptional and post-transcriptional levels. The net abundance of mature mRNA species in cells is determined by the balance between ...
Measurement of Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in Culture Cells for Assessment of the Energy Metabolism
测定培养细胞中的消氧率(OCR)和细胞外酸化率(ECAR)以评估能量代谢
作者:Birte Plitzko and Sandra Loesgen日期:05/20/2018,浏览量:56327,Q&A: 0
Mammalian cells generate ATP by mitochondrial (oxidative phosphorylation) and non-mitochondrial (glycolysis) metabolism. Cancer cells are known to reprogram their metabolism using different ...
Microbial Mutagenicity Assay: Ames Test
微生物诱变分析:Ames试验
作者:Urvashi Vijay, Sonal Gupta, Priyanka Mathur, Prashanth Suravajhala and Pradeep Bhatnagar日期:03/20/2018,浏览量:25968,Q&A: 2
The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to ...
Stereotaxic Adeno-associated Virus Injection and Cannula Implantation in the Dorsal Raphe Nucleus of Mice
在小鼠中缝背核立体定向注射腺相关病毒和埋植套管
作者:Patrícia A. Correia, Sara Matias and Zachary F. Mainen日期:09/20/2017,浏览量:13476,Q&A: 1
Optogenetic methods are now widespread in neuroscience research. Here we present a detailed surgical procedure to inject adeno-associated viruses and implant optic fiber cannulas in the dorsal raphe ...
Superoxide Dismutase (SOD) and Catalase (CAT) Activity Assay Protocols for Caenorhabditis elegans
秀丽隐杆线虫中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性测定实验方案
作者:Jing Zhang, Rui Chen, Zhenyang Yu and Lili Xue日期:08/20/2017,浏览量:30098,Q&A: 0
Assays for superoxide dismutase (SOD) and catalase (CAT) activities are widely employed to indicate antioxidant responses underlying the toxic effects of test chemicals. Yet, earlier studies mainly ...
Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords
小鼠脑和脊髓中可溶性和不溶性蛋白质组分的提取
作者:Oliver Wirths日期:08/05/2017,浏览量:21282,Q&A: 1
The current protocol details the preparation of soluble and insoluble protein lysates from mouse brain or spinal cord samples. In detail, tissue homogenization and sequential protein extraction are ...
Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader
使用荧光读板仪通过钙指示剂Fura-2 AM 测定细胞内钙浓度
作者:Magdiel Martínez, Namyr A. Martínez and Walter I. Silva日期:07/20/2017,浏览量:25793,Q&A: 1
Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to ...
BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
显微镜观察BODIPY 493/503 染色中性脂滴以及用流式细胞仪量化
作者:Bo Qiu and M. Celeste Simon日期:09/05/2016,浏览量:45670,Q&A: 1
Lipid droplets (LDs) are ubiquitous, dynamic organelles and function as a storage depot for neutral lipids, including triglycerides and cholesterol esters (Walther and Farese, 2012). The movement of ...
Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii
鲍氏不动杆菌的最小抑菌浓度(MIC)测定
作者:Ming-Feng Lin, Yun-You Lin and Chung-Yu Lan日期:12/05/2014,浏览量:43314,Q&A: 3
Minimal inhibition concentration (MIC) is the lowest concentration of an antimicrobial agent that can inhibit the visible growth of a microorganism after overnight incubation. MIC determination is ...
Dimethylmethylene Blue Assay (DMMB)
二甲基亚甲基蓝试验(DMMB)
作者:Vivien Jane Coulson- Thomas and Tarsis Ferreira Gesteira日期:09/20/2014,浏览量:49965,Q&A: 8
Glycosaminoglycans (GAGs) are long unbranched polysaccharides consisting of repeating disaccharide units composed of a hexosamine (glucosamine or galactosamine) and a hexuronic acid (glucuronic or ...