Whole-genome Methylation Analysis of APOBEC Enzyme-converted DNA (~5 kb) by Nanopore Sequencing
通过纳米孔测序对 APOBEC 酶转化 DNA (~5 kb) 进行全基因组甲基化分析
作者:Suzuko Zaha, Yoshitaka Sakamoto, Satoi Nagasawa, Sumio Sugano, Ayako Suzuki, Yutaka Suzuki and Masahide Seki日期:03/05/2022,浏览量:2668,Q&A: 0

In recent years, DNA methylation research has been accelerated by the advent of nanopore sequencers. However, read length has been limited by the constraints of base conversion using the bisulfite

Labeling Endogenous Proteins Using CRISPR-mediated Insertion of Exon (CRISPIE)
使用 CRISPR 介导的外显子插入 (CRISPIE) 标记内源性蛋白质
作者:Evan A. Wilson, Tianyi Mao and Haining Zhong日期:03/05/2022,浏览量:2968,Q&A: 0

The CRISPR/Cas9 technology has transformed our ability to edit eukaryotic genomes. Despite this breakthrough, it remains challenging to precisely knock-in large DNA sequences, such as those encoding

Labeling and Tracking Mitochondria with Photoactivation in Drosophila Embryos
作者:Sayali Chowdhary and Richa Rikhy日期:03/05/2022,浏览量:1497,Q&A: 0

Mitochondria are relatively small, fragmented, and abundant in the large embryos of Drosophila, Xenopus and zebrafish. It is essential to study their distribution and dynamics in these embryos to

An in vitro Blood-brain Barrier Model to Study the Penetration of Nanoparticles
作者:Sezin Aday, Wen Li, Jeffrey M. Karp and Nitin Joshi日期:02/20/2022,浏览量:3505,Q&A: 0

The blood-brain barrier (BBB), a crucial protection mechanism in the central nervous system (CNS), is a selective barrier comprised of endothelial cells. It hampers the development of therapeutic and

Optimization of Extracellular Flux Assay to Measure Respiration of Anchorage-independent Tumor Cell Spheroids
Three-dimensional (3D) cell culture models are widely used in tumor studies to more accurately reflect cell-cell interactions and tumor growth conditions in vivo. 3D anchorage-independent ...
NBD-lipid Uptake Assay for Mammalian Cell Lines
哺乳动物细胞系的 NBD 脂质摄取测定
作者:Sara Abad Herrera, Marta Grifell-Junyent and Thomas Günther Pomorski日期:02/20/2022,浏览量:1720,Q&A: 0

All eukaryotic cells are equipped with transmembrane lipid transporters, which are key players in membrane lipid asymmetry, vesicular trafficking, and membrane fusion. The link between mutations in

Developing Clinically Relevant Acquired Chemoresistance Models in Epithelial Ovarian Cancer Cell Lines
作者:Priti S. Shenoy, Sourav Chakraborty, Snehal M. Gaikwad, Asmita Sakpal and Pritha Ray日期:02/05/2022,浏览量:1573,Q&A: 0

Chemoresistance, the ability of cancer cells to overcome therapeutic interventions, is an area of active research. Studies on intrinsic and acquired chemoresistance have partly succeeded in

Large-scale Analysis of Sleep in Zebrafish
作者:Daniel A. Lee, Grigorios Oikonomou and David A. Prober日期:02/05/2022,浏览量:1668,Q&A: 0

Over the past decade, zebrafish have emerged as a powerful model for the study of vertebrate sleep and wake behaviors. Experimental evidence has demonstrated behavioral, anatomical, genetic, and

ATAC Sequencing Protocol For Cryopreserved Mammalian Cells
作者:Juan Manuel Caravaca, Monika Mehta, Sujatha Gowda and Bao Tran日期:01/20/2022,浏览量:1663,Q&A: 0

ATAC-seq (assay for transposase-accessible chromatin with high-throughput sequencing) is a powerful method to evaluate chromatin accessibility and nucleosome positioning at a genome-wide scale. This

Heterologous Expression and High Degree Purification of the Restriction Endonuclease SauUSI
作者:Vinayak Sadasivam Tumuluri and Kayarat Saikrishnan日期:01/05/2022,浏览量:1686,Q&A: 1

Mechanisms that target and destroy foreign nucleic acids are major barriers to horizontal gene transfer (HGT) in prokaryotes. Amongst them, restriction-modification (R-M) systems are found in ≥75%

Total Nucleic Acid Extraction from Single Zebrafish Embryos for Genotyping and RNA-seq
从单个斑马鱼胚胎中提取总核酸用于基因分型和 RNA-seq
作者:Neha Wali, Munise Merteroglu, Richard J. White and Elisabeth M. Busch-Nentwich日期:01/05/2022,浏览量:1731,Q&A: 0

RNA sequencing allows for the quantification of the transcriptome of embryos to investigate transcriptional responses to various perturbations (e.g., mutations, infections, drug treatments). Previous

A Simple Technique for Direct Immobilization of Target Enzymes from Cell Lysates Based on the SpyTag/SpyCatcher Spontaneous Reaction
基于 SpyTag/SpyCatcher 自发反应从细胞裂解物中直接固定目标酶的简单技术
作者:Li-xi Cai, Yuan-qing Lin, Yun-meng Chu, Xiao-pin Chen, Li-xing Liu, Ming Zhang and Guang-ya Zhang日期:01/05/2022,浏览量:1628,Q&A: 0

Many of the current methods for enzyme purification and immobilization suffer from several drawbacks, such as requiring tedious multistep procedures or long preparation, and being environmentally

Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation
作者:Jinyun Wu, Yuqi Cai, Xiaoyang Wu, Yue Ying, Yilin Tai and Miao He日期:03/05/2021,浏览量:6133,Q&A: 0
Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into ...
Measurement of Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in Culture Cells for Assessment of the Energy Metabolism
作者:Birte Plitzko and Sandra Loesgen日期:05/20/2018,浏览量:63346,Q&A: 1
Mammalian cells generate ATP by mitochondrial (oxidative phosphorylation) and non-mitochondrial (glycolysis) metabolism. Cancer cells are known to reprogram their metabolism using different ...
Isolation, Culture and Differentiation of Adult Hippocampal Precursor Cells
作者:Stefanie N. Bernas, Odette Leiter, Tara L. Walker and Gerd Kempermann日期:11/05/2017,浏览量:16257,Q&A: 0
There are two neurogenic niches in the adult mammalian brain: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas can be ...
Targeted Nucleotide Substitution in Mammalian Cell by Target-AID
作者:Takayuki Arazoe, Keiji Nishida and Akihiko Kondo日期:06/05/2017,浏览量:16372,Q&A: 0
Programmable RNA-guided nucleases based on CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) systems have been applied to various type of cells as ...
Sucrose Preference Test to Measure Anhedonic Behaviour in Mice
作者:Tsvetan Serchov, Dietrich van Calker and Knut Biber日期:10/05/2016,浏览量:30903,Q&A: 0
The sucrose preference test (SPT) is a reward-based test, used as in indicator of anhedonia. Anhedonia, or the decreased ability to experience pleasure, represents one of the core symptoms of ...