热门实验
Evaluation of the Sequence Variability within the PCR Primer/Probe Target Regions of the SARS-CoV-2 Genome
SARS-CoV-2基因组PCR引物/探针靶区序列变异性评价
作者:Kashif Aziz Khan and Peter Cheung日期:12/20/2020,浏览量:1306,Q&A: 0
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; initially named 2019-nCoV) is responsible for the recent coronavirus disease (COVID-19) pandemic, and polymerase chain reaction (PCR) is ...
Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
农杆菌介导的优良籼稻品种Komboka的高效转化
Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, ...
Confocal and Super-resolution Imaging of RNA in Live Bacteria Using a Fluorogenic Silicon Rhodamine-binding Aptamer
含氟硅-罗丹明适配体用于活性菌中RNA的激光共聚焦超分辨率成像
作者:Regina Wirth, Peng Gao, G. Ulrich Nienhaus, Murat Sunbul and Andres Jäschke日期:05/05/2020,浏览量:3734,Q&A: 0
Genetically encoded light-up RNA aptamers have been shown to be promising tools for the visualization of RNAs in living cells, helping us to advance our understanding of the broad and complex life of ...
Single Cell Migration Assay Using Human Breast Cancer MDA-MB-231 Cell Line
利用人乳腺癌MDA-MB-231细胞系进行单细胞迁移分析
作者:David M. Gau and Partha Roy日期:04/20/2020,浏览量:2970,Q&A: 0
Cell migration is a fundamental cellular process that plays a crucial role in many physioglogical and pathological processes such as wound healing or cancer metastasis. Many assays have been ...
Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA
通过合成共价闭合环状(3Cs)DNA构建无偏和专用CRISPR / Cas gRNA文库
作者:Martin Wegner, Koraljka Husnjak and Manuel Kaulich日期:01/05/2020,浏览量:3753,Q&A: 0
Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus ...
Tryptophan Fluorescence Quenching Assays for Measuring Protein-ligand Binding Affinities: Principles and a Practical Guide
用于检测蛋白配体结合亲和力的色氨酸荧光猝灭试验:原理和操作指南
作者:Anthony Yammine, Jinlong Gao and Ann H. Kwan日期:06/05/2019,浏览量:11368,Q&A: 0
Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a ...
Straight Channel Microfluidic Chips for the Study of Platelet Adhesion under Flow
直通道微流控芯片用于研究流动中的血小板黏附
作者:Alexander Dupuy, Lining Arnold Ju and Freda H Passam日期:03/20/2019,浏览量:8545,Q&A: 0
Microfluidic devices have become an integral method of cardiovascular research as they enable the study of shear force in biological processes, such as platelet function and thrombus formation. ...
Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
作者:Rachna Narayanan and Andrew C. Oates日期:03/20/2019,浏览量:8920,Q&A: 0
In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current ...
Induced Germinal Center B Cell Culture System
诱导生发中心B细胞培养系统
作者:Kei Haniuda and Daisuke Kitamura日期:02/20/2019,浏览量:8261,Q&A: 0
The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate ...
Induction and Analysis of Anti-CD40-induced Colitis in Mice
小鼠中抗-CD40抗体诱导的结肠炎的诱导和分析
作者:Barbara Joyce-Shaikh, Daniel J. Cua and David Bauché日期:02/05/2019,浏览量:5927,Q&A: 0
Colon inflammation or colitis affects more than 1 million people worldwide. Several pre-clinical models, including chemical-induced (i.e., DSS, TNBS) or pathogen-induced (i.e., ...
Evaluation of Anticancer activity of Silver Nanoparticles on the A549 Human Lung Carcinoma Cell Lines through Alamar Blue Assay
通过阿尔玛蓝显色法评估A549人肺癌细胞系上的银纳米粒子
作者:Nikita Sharma, Geeta Arya, R. Mankamna Kumari, Nidhi Gupta and Surendra Nimesh日期:01/05/2019,浏览量:8379,Q&A: 0
Silver nanoparticles have been widely studied to possess antimicrobial as well as anticancer activity, and have found its applications in various fields including pharmaceutical industry, ...
Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe
用阳离子JC-1染料作为敏感荧光探针进行线粒体膜电势分析
作者:Farzane Sivandzade, Aditya Bhalerao and Luca Cucullo日期:01/05/2019,浏览量:13871,Q&A: 0
In recent years, fluorescent dyes have been frequently used for monitoring mitochondrial membrane potential to evaluate mitochondrial viability and function. However, the reproducibility of the ...
Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells
利用单分子荧光原位杂交进行贴壁动物细胞中RNA的检测
作者:Gal Haimovich and Jeffrey E. Gerst日期:11/05/2018,浏览量:21869,Q&A: 1
Transcription and RNA decay play critical roles in the process of gene expression and the ability to accurately measure cellular mRNA levels is essential for understanding this regulation. Here, we ...
mRNA Stability Assay Using Transcription Inhibition by Actinomycin D in Mouse Pluripotent Stem Cells
利用放线菌素D抑制转录检测小鼠多能干细胞中mRNA的稳定性
作者:Madara Ratnadiwakara and Minna-Liisa Änkö日期:11/05/2018,浏览量:19294,Q&A: 2
Gene expression is regulated through multiple steps at both transcriptional and post-transcriptional levels. The net abundance of mature mRNA species in cells is determined by the balance between ...
Cell Synchronization by Double Thymidine Block
用胸腺嘧啶核苷双阻断法同步细胞
作者:Guo Chen and Xingming Deng日期:09/05/2018,浏览量:23112,Q&A: 1
Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine ...
Isothermal Titration Calorimetry: A Biophysical Method to Characterize the Interaction between Label-free Biomolecules in Solution
等温滴定量热法:一种检测溶液中非标记生物分子相互作用的生物物理方法
作者:Andrea Saponaro日期:08/05/2018,浏览量:16895,Q&A: 0
This protocol can be applied to analyze the direct interaction between a soluble protein and a target ligand molecule using Isothermal Titration Calorimetry (ITC, Malvern). ITC allows the biophysical ...
Measurement of Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in Culture Cells for Assessment of the Energy Metabolism
测定培养细胞中的消氧率(OCR)和细胞外酸化率(ECAR)以评估能量代谢
作者:Birte Plitzko and Sandra Loesgen日期:05/20/2018,浏览量:53923,Q&A: 0
Mammalian cells generate ATP by mitochondrial (oxidative phosphorylation) and non-mitochondrial (glycolysis) metabolism. Cancer cells are known to reprogram their metabolism using different ...
Transmission Electron Microscopy for Analysis of Mitochondria in Mouse Skeletal Muscle
使用透射电子显微镜技术分析小鼠骨骼肌线粒体
作者:Joseph D. McMillan and Michael A. Eisenback日期:05/20/2018,浏览量:12343,Q&A: 0
Skeletal muscle is the most abundant tissue in the human body and regulates a variety of functions including locomotion and whole-body metabolism. Skeletal muscle has a plethora of mitochondria, the ...
Generation of Luciferase-expressing Tumor Cell Lines
表达萤光素酶的肿瘤细胞系的生成
作者:Todd V. Brennan, Liwen Lin, Xiaopei Huang and Yiping Yang日期:04/20/2018,浏览量:12721,Q&A: 1
Murine tumor models have been critical to advances in our knowledge of tumor physiology and for the development of effective tumor therapies. Essential to these studies is the ability to both track ...
Detection and Analysis of Circular RNAs by RT-PCR
采用RT-PCR检测和分析环状RNA
作者:Amaresh C Panda and Myriam Gorospe日期:03/20/2018,浏览量:13331,Q&A: 4
Gene expression in eukaryotic cells is tightly regulated at the transcriptional and posttranscriptional levels. Posttranscriptional processes, including pre-mRNA splicing, mRNA export, mRNA turnover, ...
Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9
使用CRISPR-Cas9在酵母中进行单步精确基因组编辑
Genome modification in budding yeast has been extremely successful largely due to its highly efficient homology-directed DNA repair machinery. Several methods for modifying the yeast genome have ...
Quantification of Plant Cell Death by Electrolyte Leakage Assay
通过电解质渗漏测定法定量植物细胞死亡
作者:Noriyuki Hatsugai and Fumiaki Katagiri日期:03/05/2018,浏览量:16080,Q&A: 1
We describe a protocol to measure the electrolyte leakage from plant tissues, resulting from loss of cell membrane integrity, which is a common definition of cell death. This simple protocol is ...
Adapting the Smart-seq2 Protocol for Robust Single Worm RNA-seq
改编Smart-seq2实验方案以实现稳定的单蠕虫RNA测序
Most nematodes are small worms that lack enough RNA for regular RNA-seq protocols without pooling hundred to thousand of individuals. We have adapted the Smart-seq2 protocol in order to sequence the ...
Isolation and Establishment of Mesenchymal Stem Cells from Wharton’s Jelly of Human Umbilical Cord
从人脐带华通氏胶中分离及建立间充质干细胞
作者:Umesh Goyal, Chitra Jaiswal and Malancha Ta日期:02/20/2018,浏览量:12817,Q&A: 4
Mesenchymal stem cells (MSCs) are currently considered as ‘medicinal signaling cells’ and a promising resource in regard to cell-based regenerative therapy. Umbilical cord is a human term perinatal ...
Obtaining Acute Brain Slices
急性脑组织切片的制备
作者:Thomas Papouin and Philip G. Haydon日期:01/20/2018,浏览量:15764,Q&A: 1
Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step ...
Multiple Stepwise Gene Knockout Using CRISPR/Cas9 in Escherichia coli
使用CRISPR/Cas9在大肠埃希杆菌中进行逐步多基因敲除
作者:Enrico König, Francesca Zerbini, Ilaria Zanella, Davide Fraccascia and Guido Grandi日期:01/20/2018,浏览量:20331,Q&A: 1
With the recent implementation of the CRISPR/Cas9 technology as a standard tool for genome editing, laboratories all over the world are undergoing one of the biggest advancements in molecular biology ...
Targeted Nucleotide Substitution in Mammalian Cell by Target-AID
在哺乳动物细胞中通过靶向性AID进行核苷酸靶向置换
作者:Takayuki Arazoe, Keiji Nishida and Akihiko Kondo日期:06/05/2017,浏览量:11382,Q&A: 0
Programmable RNA-guided nucleases based on CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) systems have been applied to various type of cells as ...
Chromatin Immunoprecipitation Experiments from Whole Drosophila Embryos or Larval Imaginal Discs
整个果蝇胚胎或幼虫成虫盘的染色质免疫沉淀实验
作者:Vincent Loubiere, Anna Delest, Bernd Schuettengruber, Anne-Marie Martinez and Giacomo Cavalli日期:06/05/2017,浏览量:9546,Q&A: 1
Chromatin Immunoprecipitation coupled either to qPCR (qChIP) or high-throughput sequencing (ChIP-Seq) has been extensively used in the last decades to identify the DNA binding sites of transcription ...
Muscle Histology Characterization Using H&E Staining and Muscle Fiber Type Classification Using Immunofluorescence Staining
H&E染色鉴定肌肉组织学特征并通过免疫荧光染色进行肌纤维分类
作者:Chao Wang, Feng Yue and Shihuan Kuang日期:05/20/2017,浏览量:18419,Q&A: 1
Muscle function is determined by its structure and fiber type composition. Here we describe a protocol to examine muscle histology and myofiber types using hematoxylin and eosin (H&E) and ...
Murine Bronchoalveolar Lavage
小鼠支气管肺泡灌洗
作者:Fan Sun, Gutian Xiao and Zhaoxia Qu日期:05/20/2017,浏览量:19136,Q&A: 1
A basic Bronchoalveolar lavage (BAL) procedure in mouse is described here. Cells and fluids obtained from BAL can be analyzed by Hema3-staining, immunostaining, Fluorescence-activated cell sorting ...
Robust Generation of Knock-in Cell Lines Using CRISPR-Cas9 and rAAV-assisted Repair Template Delivery
使用CRISPR-Cas9和rAAV辅助的修复模板递送稳定生成敲入细胞系
作者:Giel Vandemoortele, Delphine De Sutter and Sven Eyckerman日期:04/05/2017,浏览量:17488,Q&A: 0
The programmable Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated nuclease 9 (Cas9) technology revolutionized genome editing by providing an efficient way to cut the ...
Polysome Fractionation to Analyze mRNA Distribution Profiles
多聚核糖体分馏分析mRNA分布特征
作者:Amaresh C. Panda, Jennifer L. Martindale and Myriam Gorospe日期:02/05/2017,浏览量:21184,Q&A: 5
Eukaryotic cells adapt to changes in external or internal signals by precisely modulating the expression of specific gene products. The expression of protein-coding genes is controlled at the ...
Dot Blot Analysis of N6-methyladenosine RNA Modification Levels
N6-甲基腺苷RNA修饰水平的斑点印迹分析
作者:Lisha Shen, Zhe Liang and Hao Yu日期:01/05/2017,浏览量:20043,Q&A: 3
N6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). The total amount of m6A can be detected by several methods, ...
Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
利用磺酰罗丹明B(SRB)法检测细胞的增殖
作者:Esteban A. Orellana and Andrea L. Kasinski日期:11/05/2016,浏览量:39285,Q&A: 0
The SRB assay has been used since its development in 1990 (Skehan et al., 1990) to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies (Vichai ...
BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
显微镜观察BODIPY 493/503 染色中性脂滴以及用流式细胞仪量化
作者:Bo Qiu and M. Celeste Simon日期:09/05/2016,浏览量:44437,Q&A: 1
Lipid droplets (LDs) are ubiquitous, dynamic organelles and function as a storage depot for neutral lipids, including triglycerides and cholesterol esters (Walther and Farese, 2012). The movement of ...
Hemagglutination Inhibition (HI) Assay of Influenza Viruses with Monoclonal Antibodies
单克隆抗体介导的流感病毒血细胞凝集抑制(HI)试验
Heamagglutination is inhibited when antibodies are present because antibodies to the influenza virus will prevent attachment of the virus to red blood cells. The highest dilution of antibody that ...
Differentiation of THP1 Cells into Macrophages for Transwell Co-culture Assay with Melanoma Cells
THP1细胞分化成巨噬细胞及与黑色素瘤细胞的跨室联合培养试验
作者:Michael Peter Smith, Helen Young, Adam Hurlstone and Claudia Wellbrock日期:11/05/2015,浏览量:36909,Q&A: 2
Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer treatments move towards combing both targeted- and immuno-therapies in new treatment ...
Analysis of Protein Stability by the Cycloheximide Chase Assay
放线菌酮追踪检测分析蛋白质的稳定性
Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly ...
Infectious Focus Assays and Multiplicity of Infection (MOI) Calculations for Alpha-herpesviruses
α疱疹病毒的疫源地分析和感染复数(MOI)计算
作者:Anna Sloutskin and Ronald S. Goldstein日期:11/20/2014,浏览量:37123,Q&A: 0
Titration of viral stocks is a critical process before any experimental use of the virus. Here we describe an infectious focus assay for several alphaherpesviruses, a titration method for ...
Novel Object Recognition for Studying Memory in Mice
采用新物体识别研究小鼠的记忆
作者:Tzyy-Nan Huang and Yi-Ping Hsueh日期:10/05/2014,浏览量:27943,Q&A: 0
Memory tests are important indexes of the brain functions for rodents behavior assay. Many memory tasks require external forces (e.g. electric shocks) or intrinsic forces (e.g. ...
Targeted Gene Mutation in Rice Using a CRISPR-Cas9 System
采用CRISPR-Cas9 系统进行水稻基因定点突变
作者:Kabin Xie, Bastian Minkenberg and Yinong Yang日期:09/05/2014,浏览量:27944,Q&A: 0
RNA-guided genome editing (RGE) using bacterial type II cluster regularly interspaced short palindromic repeats (CRISPR)–associated nuclease (Cas) has emerged as a simple and versatile tool for ...
In vitro Protein Ubiquitination Assays
体外蛋白质泛素化修饰试验
作者:Qingzhen Zhao and Qi Xie日期:10/05/2013,浏览量:39274,Q&A: 1
Ubiquitin can be added to substrate protein as a protein tag by the concerted actions of ubiquitin activating enzyme (E1), ubiquitin conjugating enzyme (E2) and ubiquitin protein ligase (E3). At the ...
Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation
采用Percoll密度梯度离心法分离小鼠肿瘤浸润性白细胞
作者:Ying Liu, Keqiang Chen, Chunyan Wang, Wanghua Gong, Teizo Yoshimura, Ji Ming Wang and Mingyong Liu日期:09/05/2013,浏览量:36329,Q&A: 4
A hallmark of cancer-associated inflammation is the infiltration of leukocytes into tumors, which is believed to be recruited by chemokines. Some infiltrating leukocytes such as macrophages often ...
ImmunoPrecipitation of Nuclear Protein with Antibody Affinity Columns
采用抗体亲和色谱进行细胞核蛋白免疫沉淀试验
作者:Saïda Dadi, Dominique Payet-Bornet and Pierre Ferrier日期:02/05/2013,浏览量:30891,Q&A: 1
Co-Immunoprecipitation (Co-IP) is the method used to pull down protein partners of a protein of interest using an antibody that specifically binds to this specific protein in order to test ...
Scratch Wound Healing Assay
划痕实验
作者:Yanling Chen日期:03/05/2012,浏览量:113231,Q&A: 6
The scratch wound healing assay has been widely adapted and modified to study the effects of a variety of experimental conditions, for instance, gene knockdown or chemical exposure, on mammalian cell ...
Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell
Transwell小室检测人乳腺上皮癌细胞迁移
作者:Yanling Chen日期:02/20/2012,浏览量:66542,Q&A: 7
Transwell migration assays have been widely used for studying the motility of different types of cells including metastatic cancer cells. The assay is also useful in screens for compounds that act as ...