高引方法
Semi-quantitative Determination of Protein Expression Using Immunohistochemistry Staining and Analysis: An Integrated Protocol
一种综合的实验方法:利用免疫组织化学染色和分析进行蛋白表达的半定量测定
作者:Alexandra R Crowe and Wei Yue日期:12/20/2019,浏览量:9164,Q&A: 0
Semi-quantitative IHC is a powerful method for investigating protein expression and localization within tissues. The semi-quantitative immunohistochemistry (IHC) involves using software such as free ...
Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe
用阳离子JC-1染料作为敏感荧光探针进行线粒体膜电势分析
作者:Farzane Sivandzade, Aditya Bhalerao and Luca Cucullo日期:01/05/2019,浏览量:23203,Q&A: 0
In recent years, fluorescent dyes have been frequently used for monitoring mitochondrial membrane potential to evaluate mitochondrial viability and function. However, the reproducibility of the ...
Detection and Analysis of Circular RNAs by RT-PCR
采用RT-PCR检测和分析环状RNA
作者:Amaresh C Panda and Myriam Gorospe日期:03/20/2018,浏览量:20666,Q&A: 5
Gene expression in eukaryotic cells is tightly regulated at the transcriptional and posttranscriptional levels. Posttranscriptional processes, including pre-mRNA splicing, mRNA export, mRNA turnover, ...
DQ-Red BSA Trafficking Assay in Cultured Cells to Assess Cargo Delivery to Lysosomes
在培养细胞中评估物质运输至溶酶体的DQ-Red BSA转运分析法
作者:Rituraj Marwaha and Mahak Sharma日期:10/05/2017,浏览量:18996,Q&A: 1
Lysosomes are the terminal end of the endocytic pathway having acidic environment required for active hydrolases that degrade the cargo delivered to these compartments. This process of cargo delivery ...
Polysome Fractionation to Analyze mRNA Distribution Profiles
多聚核糖体分馏分析mRNA分布特征
作者:Amaresh C. Panda, Jennifer L. Martindale and Myriam Gorospe日期:02/05/2017,浏览量:26016,Q&A: 5
Eukaryotic cells adapt to changes in external or internal signals by precisely modulating the expression of specific gene products. The expression of protein-coding genes is controlled at the ...
Detection of Reactive Oxygen Species in Oryza sativa L. (Rice)
水稻中活性氧的检测
作者:Navdeep Kaur, Isha Sharma, Kamal Kirat and Pratap Kumar Pati日期:12/20/2016,浏览量:18151,Q&A: 2
Superoxide ions (O2-) and hydrogen peroxide (H2O2) are the reactive oxygen species (ROS) that play a significant role in regulation of many plant processes. The level ...
Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
利用磺酰罗丹明B(SRB)法检测细胞的增殖
作者:Esteban A. Orellana and Andrea L. Kasinski日期:11/05/2016,浏览量:43359,Q&A: 0
The SRB assay has been used since its development in 1990 (Skehan et al., 1990) to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies (Vichai ...
Protocol for Primary Microglial Culture Preparation
原代小胶质细胞培养制备法
作者:Hong Lian, Ethan Roy and Hui Zheng日期:11/05/2016,浏览量:20102,Q&A: 0
Primary microglia, in either mono-culture or co-culture with neurons or astrocytes, are a powerful tool for studying mechanisms underlying microglial inflammatory responses and cell type-specific ...
BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
显微镜观察BODIPY 493/503 染色中性脂滴以及用流式细胞仪量化
作者:Bo Qiu and M. Celeste Simon日期:09/05/2016,浏览量:51355,Q&A: 1
Lipid droplets (LDs) are ubiquitous, dynamic organelles and function as a storage depot for neutral lipids, including triglycerides and cholesterol esters (Walther and Farese, 2012). The movement of ...
Collagen-induced Arthritis: A Model for Murine Autoimmune Arthritis
胶原蛋白诱导的关节炎:构建鼠自身免疫性关节炎的模型
作者:Kathryn M. Pietrosimone, Mengyao Jin, Brad Poston and Peng Liu日期:10/20/2015,浏览量:13023,Q&A: 1
Collagen-induced arthritis (CIA) is a common autoimmune animal model used to study rheumatoid arthritis (RA). The development of CIA involves infiltration of macrophages and neutrophils into the ...
Pyridine Hemochromagen Assay for Determining the Concentration of Heme in Purified Protein Solutions
吡啶血色原法测定纯化蛋白溶液中血红素的浓度
作者:Ian Barr and Feng Guo日期:09/20/2015,浏览量:29170,Q&A: 2
Heme is a common cofactor in proteins, found in hemoglobin, myoglobin, cytochrome P450, DGCR8, and nitric oxide synthase, among others. This protocol describes a method for quantifying heme that ...
Cryo-focused Ion Beam Sample Preparation for Imaging Vitreous Cells by Cryo-electron Tomography
低温聚焦离子束方法制备样本用于低温电子断层扫描术观察玻璃化细胞
作者:Miroslava Schaffer, Benjamin D. Engel, Tim Laugks, Julia Mahamid, Jürgen M. Plitzko and Wolfgang Baumeister日期:09/05/2015,浏览量:19634,Q&A: 0
Cryo-electron tomography (CET) is a well-established technique for imaging cellular and molecular structures at sub-nanometer resolution. As the method is limited to samples that are thinner than 500 ...
Measurement of Chlorophyll a and Carotenoids Concentration in Cyanobacteria
蓝藻细菌中叶绿素a和类胡萝卜素浓度的测量
作者:Tomáš Zavřel, Maria A. Sinetova and Jan Červený日期:05/05/2015,浏览量:31308,Q&A: 2
This is a protocol for precise measurement of chlorophyll a and total carotenoid concentrations in cyanobacteria cells. Cellular chlorophyll concentration is one of the central physiological ...
Analysis of Protein Stability by the Cycloheximide Chase Assay
放线菌酮追踪检测分析蛋白质的稳定性
Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly ...
Hypoxia Studies with Pimonidazole in vivo
体内哌莫硝唑缺氧研究
作者:Kristina Y. Aguilera and Rolf A. Brekken日期:10/05/2014,浏览量:24352,Q&A: 0
Therapy-induced hypoxia drives changes in the tumor microenvironment that contribute to the poor response to therapy. Hypoxia is capable of driving the expression and/or activation of specific ...
Histochemical Detection of Superoxide and H2O2 Accumulation in Brassica juncea Seedlings
芥菜幼苗中超氧化物和H2O2累积的组织化学检测
作者:Deepak Kumar, Mohd Aslam Yusuf, Preeti Singh, Meryam Sardar and Neera Bhalla Sarin日期:04/20/2014,浏览量:29570,Q&A: 3
Plant cells continually produce reactive oxygen species (ROS) as a by-product of aerobic metabolism. Increased production of ROS occurs under unfavorable conditions imposed by various abiotic and ...
Isolation of Mouse Embryo Fibroblasts
小鼠胚胎成纤维细胞的分离
作者:Marian E Durkin, Xiaolan Qian, Nicholas C Popescu and Douglas R Lowy日期:09/20/2013,浏览量:17541,Q&A: 0
Preparation of primary cultures of embryo fibroblasts from genetically engineered mouse strains can provide a valuable resource for analyzing the consequences of genetic alterations at the cellular ...
Analysis of Malondialdehyde, Chlorophyll Proline, Soluble Sugar, and Glutathione Content in Arabidopsis seedling
拟南芥中丙二醛、叶绿素脯氨酸、可溶性糖和谷胱甘肽含量的分析
作者:Zhijin Zhang and Rongfeng Huang日期:07/20/2013,浏览量:30437,Q&A: 2
The protocol has four sub-protocols, which are about the measurement of malondialdehyde, chlorophyll proline, soluble sugar, and glutathione content, respectively, in Arabidopsis seedling by ...
Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves
拟南芥叶中过氧化氢的检测(DAB染色法)
作者:Arsalan Daudi and Jose A. O’Brien日期:09/20/2012,浏览量:74899,Q&A: 22
In this protocol, the in situ detection of hydrogen peroxide (one of several reactive oxygen species) is described in mature Arabidopsis rosette leaves by staining with ...