联合评审(
Akira Karasawa New York Structural Biology Center
8 protocols

Alba Blesa Universidad Francisco de Vitoria
29 protocols

Alexandros C Kokotos Weill Cornell Medicine
18 protocols

Alka Mehra Institute of Genomics and Integrative Biology
41 protocols

编委
Ralph Thomas Boettcher
  • Project group leader, Max Planck Institute for Biochemistry
研究方向
  • 细胞生物学
  • 已发表 protocol 1
已发表 protocol 1篇
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
β1 整合素细胞表面免疫共沉淀(选择性免疫共沉淀)

作者:Ralph T. Böttcher日期:11/05/2013,浏览量:9882,Q&A: 0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
参审 protocol 6篇
Cell-free Synthesis of Correctly Folded Proteins with Multiple Disulphide Bonds: Production of Fungal Hydrophobins
有多重二硫化物键的正确折叠蛋白质的无细胞合成:真菌疏水性化合物的生产
作者:Rezwan Siddiquee and Ann H Kwan日期:05/20/2021,浏览量:2447,Q&A: 0

Cell-free synthesis is a powerful technique that uses the transcriptional and translational machinery extracted from cells to create proteins without the constraints of living cells. Here, we report a cell-free protein production protocol using

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主审 protocol 44篇
Preparation of Human Chondrocytes for Profiling Using Cytometry by Time-of-flight (cyTOF)
用飞行时间(cyTOF flight)流式细胞术制备人软骨细胞用于分析研究
作者:Fiorella Carla Grandi and Nidhi Bhutani日期:07/20/2021,浏览量:1258,Q&A: 0

Single-cell technologies have allowed high-resolution profiling of tissues and thus a deeper understanding of tissue homeostasis and disease heterogeneity. Understanding this heterogeneity can be especially important for tailoring treatments in a

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Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening
基于荧光的全染色单细胞分析和清除的微组织和类器官高通量筛选
作者:Henrik Renner, Mandy Otto, Martha Grabos, Hans R. Schöler and Jan M. Bruder日期:06/20/2021,浏览量:1679,Q&A: 1

Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues (“organoids”), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically,

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