联合评审(
Akira Karasawa New York Structural Biology Center
10 protocols

Alba Blesa Universidad Francisco de Vitoria
41 protocols

Alexandros C Kokotos Weill Cornell Medicine
18 protocols

Alka Mehra Institute of Genomics and Integrative Biology
45 protocols

编委
Ralph Thomas Boettcher
  • Project group leader, Max Planck Institute for Biochemistry
研究方向
  • 细胞生物学
  • 已发表 protocol 1
已发表 protocol 1篇
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
β1 整合素细胞表面免疫共沉淀(选择性免疫共沉淀)

作者:Ralph T. Böttcher日期:11/05/2013,浏览量:10694,Q&A: 0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
参审 protocol 6篇
Cell-free Synthesis of Correctly Folded Proteins with Multiple Disulphide Bonds: Production of Fungal Hydrophobins
有多重二硫化物键的正确折叠蛋白质的无细胞合成:真菌疏水性化合物的生产
作者:Rezwan Siddiquee and Ann H Kwan日期:05/20/2021,浏览量:3508,Q&A: 0

Cell-free synthesis is a powerful technique that uses the transcriptional and translational machinery extracted from cells to create proteins without the constraints of living cells. Here, we report a cell-free protein production protocol using

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主审 protocol 48篇
Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae
酿酒酵母内体循环囊泡的免疫分离
作者:Sho W. Suzuki and Scott D. Emr日期:05/05/2022,浏览量:878,Q&A: 0

Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes (i.e., retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further

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Single Molecule Tracking Nanoscopy Extended to Two Colors with MTT2col for the Analysis of Cell-Cell Interactions in Leukemia
使用 MTT2col 将单分子跟踪纳米显微镜扩展到两种颜色,用于分析白血病中的细胞-细胞相互作用
作者:Loriane Maillot, Magali Irla and Arnauld Sergé日期:04/20/2022,浏览量:935,Q&A: 0

Single molecule tracking (SMT) is a powerful technique to study molecular dynamics, and is particularly adapted to monitor the motion and interactions of cell membrane components. Assessing interactions among two molecular populations is classically

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