CS
Christian Schwarz
  • Molecular Plant Science, Ludwig-Maximilians-Universität, Germany
研究方向
  • Plant science
35S pulse Labelling of Chlamydomonas Chloroplast Proteins
衣藻属(Chlamydomonas )叶绿体蛋白质的35S脉冲标记
作者:Alexandra-Viola Bohne, Christian Schwarz and Joerg Nickelsen日期:06/05/2013,浏览量:9671,Q&A: 0
35S pulse labelling of proteins is used to attach a radioactive label to newly synthesized proteins, as sulfur is an element that is mainly present in proteins (Fleischmann and Rochaix 1999). Depending on your organism’s uptake mechanisms you need cysteine, methionine or sulfuric acid as a source of radioactive sulfur. This example uses Chlamydomonas cells and H235SO4 (Schwarz et al., 2012).
2D Diagonal Redox SDS-PAGE of Proteins
蛋白质二维双向还原电泳技术
作者:Christian Schwarz and Joerg Nickelsen日期:06/05/2013,浏览量:15817,Q&A: 0
2D diagonal redox SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012). Both dimensions consist of a conventional SDS-PAGE, except that the sample buffer for the first dimension lacks a reducing agent. Intermolecular disulfide bridges increase the apparent molecular weight of a protein in the first dimension, whereas intramolecular bridges decrease the apparent weight of the protein.