AV
Alan W. Varley
  • Internal Medicine, University of Texas Southwestern Medical School, USA
研究方向
  • Cell biology
Harvest and Culture of Mouse Peritoneal Macrophages
小鼠腹水中巨噬细胞的采集和培养
作者:Mingfang Lu and Alan W. Varley日期:11/20/2013,浏览量:38340,Q&A: 1
Peritoneal macrophages are used as primary macrophages in lots of studies, mainly because they are easy to obtain. Injection of thioglycollate broth i.p. induces inflammatory responses and elicits large numbers of macrophages. This protocol can be used for harvesting resident or thioglycollate-elicited peritoneal cells. Peritoneal macrophages are non-adherent in situ and when they are cultured in dishes, they become adherent so that macrophages may be separated from other types of cells in peritoneal cavity.