Shubhankar Nath
  • Senior Oncology Scientist, Bioink and Tissue Engineering, Cellink LLC
研究方向
  • Cancer biology
Photodynamic Therapy in a 3D Model of Ovarian Cancer
卵巢癌3D模型中的光动力学治疗
作者:Shubhankar Nath and Kaitlin Moore日期:08/05/2019,浏览量:4433,Q&A: 0
Photodynamic therapy (PDT), is a clinically-approved light-based anti-cancer treatment modality in which a photoactivatable photosensitizer is irradiated with an appropriate wavelength of light to generate cytotoxic molecules to kill cancer cells. In this article, we describe an in vitro PDT protocol using a 3-dimensional (3D) model of ovarian cancer that was established on beds of Matrigel. PDT was performed using a liposomal formulation of verteporfin photosensitizer (Visudyne®). The cancer cells were genetically-labeled with the fluorescent protein mCherry to facilitate the evaluation of the treatment response. This protocol is advantageous because the mCherry fluorescence is an indicator of cell viability, eliminating the need for external dyes, which often exhibit limited penetration and diffusion into 3D organoids. Additionally, Visudyne PDT achieves significant tumor-killing efficacy in a 3D model for ovarian cancer.