BS
Boris Shklyar
  • Head of Bioimaging Unit, University of Haifa Haifa
研究方向
  • Cell Biology, Developmental Biology, Neuroscience, Stem Cell, Bioimaging
Live Imaging of Phagoptosis in ex vivo Drosophila Testis
体外果蝇睾丸中吞噬作用的实时成像
作者:Diana Kanaan, Boris Shklyar, Lilach Porat-Kuperstein and Hila Toledano日期:03/20/2023,浏览量:810,Q&A: 0

Phagoptosis is a prevalent type of programmed cell death (PCD) in adult tissues in which phagocytes non-autonomously eliminate viable cells. Therefore, phagoptosis can only be studied in the context of the entire tissue that includes both the phagocyte executors and the targeted cells doomed to die. Here, we describe an ex vivo live imaging protocol of Drosophila testis to study the dynamics of phagoptosis of germ cell progenitors that are spontaneously removed by neighboring cyst cells. Using this approach, we followed the pattern of exogenous fluorophores with endogenously expressed fluorescent proteins and revealed the sequence of events in germ cell phagoptosis. Although optimized for Drosophila testis, this easy-to-use protocol can be adapted to a wide variety of organisms, tissues, and probes, thus providing a reliable and simple means to study phagoptosis.