Yugander Arra
  • Faculty, CSIR-Central Institute of Medicinal and Aromatic Plants
研究方向
  • Developmental Biology, Microbiology, Molecular Biology, Plant Science
A Step-by-step Protocol for Crossing and Marker-Assisted Breeding of Asian and African Rice Varieties
亚洲和非洲水稻品种杂交与标记辅助育种逐步指南
作者:Yugander Arra, Eliza P.-I. Loo, B.N. Devanna, Melissa Stiebner and Wolf B. Frommer日期:09/20/2024,浏览量:794,Q&A: 0

Improving desirable traits of popular rice varieties is of particular importance for small-scale food producers. Breeding is considered the most ecological and economic approach to improve yield, especially in the context of pest and pathogen-resistant varieties development. Being able to cross rice lines is also a critical step when using current transgene-based genome editing technologies, e.g., to remove transgenes. Moreover, rice breeders have developed accelerated breeding methods, including marker-assisted backcross breeding (MABB) to develop novel rice varieties with in-built resistance to biotic and abiotic stressors, grain, and nutritional quality. MABB is a highly efficient and cost-effective approach in accelerating the improvement of recipient variety by introgressing desirable traits, especially from landrace cultivars and wild rice accessions. Here, we provide a detailed protocol including video instructions for rice crossing and MABB to introgress target trait(s) of interest into the elite rice line. Further, we also highlight tips and tricks to be considered for a successful crossing and MABB.