联合作者(
Anne Ephrussi Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), Germany
2 protocols

Frank Wippich Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Germany, 69117 Germany,
1 protocol
联合评审(
Abhijit Kale Deciduous Therapeutics
10 protocols

Adam Idoine Carnegie Institution
16 protocols

Alessandro Didonna University of California, San Francisco
51 protocols

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

编委
Imre Gáspár
  • Faculty, INSTITUTE OF MOLECULAR BIOTECHNOLOGY, IMBA, Vienna
研究方向
  • 分子生物学
  • RNA localization, mRNP assembly, cytoskeletal transport,
    meiosis, chromatin remodelling during development
  • 已发表 protocol 2

教育背景

PhD, University of Szeged, 2009

实验室信息

Kikue Tachibana Lab
https://www.imba.oeaw.ac.at/research/kikue-tachibana/

发表论文

https://scholar.google.de/citations?user=I2gbMMwAAAAJ&hl=en
https://scholar.google.de/citations?user=I2gbMMwAAAAJ&hl=en
已发表 protocol 2篇
Terminal Deoxynucleotidyl Transferase Mediated Production of Labeled Probes for Single-molecule FISH or RNA Capture
末端脱氧核苷酸转移酶介导的用于单分子FISH或RNA捕获的标记探针的生成

作者:Imre Gaspar, Frank Wippich and Anne Ephrussi日期:03/05/2018,浏览量:8655,Q&A: 0
Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using ...
Ex vivo Ooplasmic Extract from Developing Drosophila Oocytes for Quantitative TIRF Microscopy Analysis
从在发育的果蝇卵母细胞中离体提取卵磷脂用于定量TIRF显微镜分析

作者:Imre Gáspár and Anne Ephrussi日期:07/05/2017,浏览量:6425,Q&A: 0
Understanding the dynamic behavior and the continuously changing composition of macromolecular complexes, subcellular structures and organelles is one of areas of active research in both cell and developmental biology, as these changes directly ...
参审 protocol 5篇
SMART (Single Molecule Analysis of Resection Tracks) Technique for Assessing DNA end-Resection in Response to DNA Damage
SMART(切除轨迹单分子分析)技术用于评估DNA末端切除对DNA损伤的反应
作者:Angela Altieri, Milena Dell’Aquila, Francesca Pentimalli, Antonio Giordano and Alfano Luigi日期:08/05/2020,浏览量:2436,Q&A: 0
DNA double strand breaks (DSBs) are among the most toxic lesions affecting genome integrity. DSBs are mainly repaired through non-homologous end joining (NHEJ) and homologous recombination (HR). A crucial step of the HR process is the generation, ...
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Application of Mechanical Forces on Drosophila Embryos by Manipulation of Microinjected Magnetic Particles
机械力应用于果蝇胚胎的磁性粒子显微注射操作
作者:Arturo D’Angelo and Jérôme Solon日期:05/05/2020,浏览量:1853,Q&A: 0
Cells generate mechanical forces to shape tissues during morphogenesis. These forces can activate several biochemical pathways and trigger diverse cellular responses by mechano-sensation, such as differentiation, division, migration and apoptosis. ...
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主审 protocol 16篇
Analyzing (Re)Capping of mRNA Using Transcript Specific 5' End Sequencing
使用转录本特异性5'端测序分析mRNA的(重新)帽化
作者:Daniel del Valle Morales and Daniel R. Schoenberg日期:10/20/2020,浏览量:1202,Q&A: 0
The 5′ cap is a ubiquitous feature of eukaryotic mRNAs. It is added in the nucleus onto newly synthesized pre-mRNA, and in the cytoplasm onto mRNAs after decapping or endonuclease cleavage. Cytoplasmic recapping can occur after loss of the cap at ...
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Fluorescence Measurement and Calibration of Intracellular pH in Starfish Oocytes
海星卵母细胞内pH值的荧光测定与校准
作者:Enako Hosoda and Kazuyoshi Chiba日期:10/05/2020,浏览量:1498,Q&A: 0
Oocyte maturation is a process wherein an oocyte arrested at prophase of meiosis I resumes meiosis to become a fertilizable egg. In starfish ovaries, a hormone released from follicle cells activates the oocytes, resulting in an increase in their ...
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