联合评审(
Fanglian He Bio-protocol
205 protocols

Hui Zhu Stanford University
18 protocols

Kate Hannan The Australian National University
15 protocols

Omar Akil University of California, San Francisco
16 protocols

Lin Fang
  • Stanford University
研究方向
  • 细胞生物学
  • 已发表 protocol 4

教育背景

PhD, University of Oregon, 2016

已发表 protocol 4篇
Antibody Purification from Western Blotting
利用免疫印迹法(WB)进行抗体纯化

作者:Lin Fang日期:03/20/2012,浏览量:19917,Q&A: 2
This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in ...
Expression and Purification of GST-tagged Proteins from E. coli
从大肠杆菌中表达和纯化GST-标签融合蛋白

作者:Lin Fang日期:09/20/2011,浏览量:20363,Q&A: 1
This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.
参审 protocol 18篇
Actin Retrograde Flow in Permeabilized Cells: Myosin-II Driven Centripetal Movement of Transverse Arcs
透性化细胞中肌动蛋白逆流:横向弧的肌球蛋白II激发的向性运动
作者:Yee Han Tee and Alexander D. Bershadsky日期:03/05/2016,浏览量:7289,Q&A: 0
Numerous biological functions such as cytokinesis, changes in cell shape and cell migration require actomyosin-driven cellular contractility. However, the detailed mechanism of how contractile forces drive cellular processes are difficult to ...
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Three Dimensional Spheroid Co-culture Invasion Assay
三维球状体共培养侵袭研究
作者:Eldo T Verghese and Thomas A Hughes日期:01/05/2015,浏览量:11366,Q&A: 0
The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells ...
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主审 protocol 77篇
Preparation of Golgi Membranes from Rat Liver
从大鼠肝中制备高尔基体膜
作者:Carmen Valente, Gabriele Turacchio, Stefania Spanò, Alberto Luini and Daniela Corda日期:11/20/2014,浏览量:7415,Q&A: 0
This protocol details the isolation of enriched Golgi membranes from rat liver, using discontinuous density gradient centrifugation. This high-yield extraction method is useful for several applications, including immunoprecipitation of solubilised ...
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Lysosomal Stability Assay
溶酶体稳定性分析
作者:Nikolaj H. T. Petersen, Thomas Kirkegaard and Marja Jäättelä日期:06/20/2014,浏览量:14975,Q&A: 0
This assay makes use of the dye Acridine Orange (AO) to determine the stability of lysosomes in living cells upon exposure to a confocal microscope laser.

AO is a lipophilic amine that readily diffuses into cells (Figure 1). Inside the ...
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