联合作者(
Margarita Salas Centro de Biología Molecular Severo Ochoa, Centro de Biología Molecular Severo Ochoa, Spain, Spain,
1 protocol

Mónica Berjón-Otero Present address: Max Planck Institute for Medical Research, Germany, Germany,
1 protocol

Ana Lechuga Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones científicas y Universidad Autónoma de Madrid, Spain, Spain,
1 protocol

María L. Salas Centro de Biología Molecular Severo Ochoa, CSIC/UAM, Spain
1 protocol
联合评审(
Adison Wong Singapore Institute of Technology
1 protocol

Alba Blesa Universidad Francisco de Vitoria
29 protocols

Anna M. Köhler Georg August Universität Göttingen
1 protocol

Arsalan Daudi University of California
157 protocols

编委
Modesto Redrejo-Rodriguez
  • Assistant Professor, Biochemistry Department, Madrid Autonomous University
研究方向
  • 分子生物学
  • DNA Replication and Repair mechanisms of viruses and mobile elements
  • 已发表 protocol 2
已发表 protocol 2篇
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
蛋白质pull-down免疫共沉淀实验分析病毒DNA结合蛋白质之间的直接相互作用

作者:Ana Lechuga, Mónica Berjón-Otero, Margarita Salas and Modesto Redrejo-Rodríguez日期:01/05/2018,浏览量:7524,Q&A: 0
This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are ...
Determination of Mutation Frequency During Viral DNA Replication
病毒DNA复制过程中的突变频率测定

作者:Modesto Redrejo-Rodríguez, Javier M. Rodríguez, Cristina Suárez and María L. Salas日期:03/20/2014,浏览量:7797,Q&A: 0
This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. ...
参审 protocol 10篇
Giant Mimiviridae CsCl Purification Protocol
拟菌病毒科巨型病毒的CsCl纯化方案
作者:Lionel Bertaux, Audrey Lartigue and Sandra Jeudy日期:11/20/2020,浏览量:1180,Q&A: 0
While different giant viruses’ purification protocols are available, they are not fully described and they use sucrose gradient that does not reach an equilibrium. Here, we report a protocol for the purification of members of the Mimiviridae ...
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Quantification of Densities of Bacterial Endosymbionts of Insects by Real-time PCR
实时PCR定量分析昆虫内共生细菌的密度
作者:Daisuke Kageyama日期:10/05/2017,浏览量:7913,Q&A: 0
Increased attention has been paid to the endosymbiotic bacteria of insects. Because most insect endosymbionts are uncultivable, quantitative PCR (qPCR) is a practical and convenient method to quantify endosymbiont titers. Here we report a protocol ...
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主审 protocol 20篇
Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
用于基因表达谱分析的基于蛋白和测序的大规模平行单细胞技术
作者:Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker日期:02/05/2019,浏览量:6039,Q&A: 0
Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
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Quantitative Transformation Efficiency Assay for Bacillus subtilis
枯草芽孢杆菌转化效率的定量测定
作者:Christian L. Loyo and Briana M. Burton日期:12/05/2018,浏览量:5562,Q&A: 0
Bacillus subtilis (B. subtilis) is a model Gram-positive organism used to study a variety of physiological states and stress responses, one of which is the development of competence. Competence refers to the physiological state of ...
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