I have completed my PhD from Prof. Deepak Saini lab, IISc. During my PhD, I conducted an extensive investigation into TCSs crosstalk and its physiological role in M. tuberculosis, employing different genetic, biochemical, and biophysical approaches.
https://sites.google.com/site/sainislab/
https://scholar.google.co.in/citations?user=TGlAkoMAAAAJ&hl=en1. Sankhe, G.D., Raja, R., Singh, D.P.*, Bheemireddy, S., Rana, S., Athira, P.J., Dixit, N.M., Saini, D.K., (2023) ‘Sequestration of histidine kinases by non-cognate response regulators establishes a threshold level of stimulation for bacterial two-component signaling’, Nature Communications,14(1), p. 4483. https://doi.org/10.1038/s41467-023-40095-2. (*joint co-first author)
2. Singh, K.K., Athira, P.J., Bhardwaj, N., Singh, D.P.*, Watson, U., Saini, D.K., (2021) ‘Acetylation of Response Regulator Protein MtrA in M. tuberculosis Regulates Its Repressor Activity’, Frontiers in Microbiology, 11, p. 516315. https://doi.org/10.3389/fmicb.2020.516315. (*co-author)
3. Hariharan, V.N., Yadav, R., Thakur, C., Singh, A., Gopinathan, R., Singh, D.P.*, Sankhe, G., Malhotra, V., Chandra, N., Bhatt, A., Saini, D.K., (2021) ‘Cyclic di‐GMP sensing histidine kinase PdtaS controls mycobacterial adaptation to carbon sources’, The FASEB Journal, 35(4). https://doi.org/10.1096/fj.202002537RR. (*co-author)
4. Singh, K., Singh, D.*, Singh, R., Saini, D., (2019) ‘In vitro and in vivo Assessment of Protein Acetylation Status in Mycobacteria’, BIO-PROTOCOL, 9(13).
https://doi.org/10.21769/BioProtoc.3291. (*co-author)
5. Emam, E.A.F., Roy, K., Singh, D.P.*, Saini, D.K., Varshney, U., 2024. An unusual activity of mycobacterial MutT1 Nudix hydrolase domain as a protein phosphatase regulates nucleoside diphosphate kinase function. J. Bacteriol. e00314-24. https://doi.org/10.1128/jb.00314-24. (*coauthor)
6. Elhassan Ali Fathi Emam, Devendra Pratap Singh*, Deepak Kumar Saini, Umesh Varshney, ‘Mycobacterial MutT1 mediated dephosphorylation of the sensor kinases reveals a new link in the regulation of the two-component signaling in bacteria’. Preprint on bioRxiv. doi: https://doi.org/10.1101/2025.03.06.641775. (*coauthor)