微生物学

分类

    现刊
    Protocol for RNA-seq Expression Analysis in Yeast
    酵母RNA-seq表达分析方法
    作者:Stefan Bohn日期:09/20/2021,浏览量:300,Q&A: 0

    Genome-wide sequencing of RNA (RNA-seq) has become an inexpensive tool to gain key insights into cellular and disease mechanisms. Sample preparation and sequencing are streamlined and allow the acquisition of hundreds of gene expression profiles in

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    An Inexpensive Imaging Platform to Record and Quantitate Bacterial Swarming
    记录和定量细菌群集的廉价成像平台
    作者:Weijie Chen, Sridhar Mani and Jay X. Tang日期:09/20/2021,浏览量:251,Q&A: 0

    Bacterial swarming refers to a rapid spread, with coordinated motion, of flagellated bacteria on a semi-solid surface (Harshey, 2003). There has been extensive study on this particular mode of motility because of its interesting biological and

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    Cell-free Translation: Preparation and Validation of Translation-competent Extracts from Saccharomyces cerevisiae
    无细胞翻译:酿酒酵母翻译活性提取物的制备和验证
    作者:Brandon M. Trainor, Anton A. Komar, Dimitri G. Pestov and Natalia Shcherbik日期:09/20/2021,浏览量:530,Q&A: 0

    Cell-free translation is a powerful technique for in vitro protein synthesis. While cell-free translation platforms prepared from bacterial, plant, and mammalian cells are commercially available, yeast-based translation systems remain proprietary

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    Intrathoracic Inoculation of Zika Virus in Aedes aegypti
    埃及伊蚊胸内接种寨卡病毒
    作者:Irma Sanchez-Vargas, Adeline E. Williams, Alexander W.E. Franz and Ken E. Olson日期:09/20/2021,浏览量:185,Q&A: 0

    Aedes aegypti mosquitoes are the main vectors of many medically relevant arthropod-borne (arbo) viruses, including Zika (ZIKV), dengue (DENV), and yellow fever (YFV). Vector competence studies with Ae. aegypti often involve challenging mosquitoes

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    Glucose Starvation, Magnesium Ion Starvation, and Bile Stress Assays
    葡萄糖饥饿、镁离子饥饿和胆汁应激分析
    作者:Aryashree Arunima and Mrutyunjay Suar日期:09/20/2021,浏览量:278,Q&A: 0

    Salmonella enterica serovar Enteritidis (S. Enteritidis) is a leading causative pathogen for food-borne gastroenteritis. During its course of infection, it confronts myriads of physiological barriers inside the host, such as nutrient deprivation,

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    Protocol for RNA-seq Expression Analysis in Yeast
    酵母RNA-seq表达分析方法
    作者:Stefan Bohn日期:09/20/2021,浏览量:300,Q&A: 0
    [Abstract]

    Genome-wide sequencing of RNA (RNA-seq) has become an inexpensive tool to gain key insights into cellular and disease mechanisms. Sample preparation and sequencing are streamlined and allow the acquisition of hundreds of gene expression profiles in a few days; however, in particular, data processing, curation, and analysis involve numerous steps

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    An Inexpensive Imaging Platform to Record and Quantitate Bacterial Swarming
    记录和定量细菌群集的廉价成像平台
    作者:Weijie Chen, Sridhar Mani and Jay X. Tang日期:09/20/2021,浏览量:251,Q&A: 0
    [Abstract]

    Bacterial swarming refers to a rapid spread, with coordinated motion, of flagellated bacteria on a semi-solid surface (Harshey, 2003). There has been extensive study on this particular mode of motility because of its interesting biological and physical relevance, e.g., enhanced antibiotic resistance (Kearns, 2010) and turbulent collective motion

    ...
    Cell-free Translation: Preparation and Validation of Translation-competent Extracts from Saccharomyces cerevisiae
    无细胞翻译:酿酒酵母翻译活性提取物的制备和验证
    作者:Brandon M. Trainor, Anton A. Komar, Dimitri G. Pestov and Natalia Shcherbik日期:09/20/2021,浏览量:530,Q&A: 0
    [Abstract]

    Cell-free translation is a powerful technique for in vitro protein synthesis. While cell-free translation platforms prepared from bacterial, plant, and mammalian cells are commercially available, yeast-based translation systems remain proprietary knowledge of individual labs. Here, we provide a detailed protocol for simple, fast, and

    ...
    Intrathoracic Inoculation of Zika Virus in Aedes aegypti
    埃及伊蚊胸内接种寨卡病毒
    作者:Irma Sanchez-Vargas, Adeline E. Williams, Alexander W.E. Franz and Ken E. Olson日期:09/20/2021,浏览量:185,Q&A: 0
    [Abstract]

    Aedes aegypti mosquitoes are the main vectors of many medically relevant arthropod-borne (arbo) viruses, including Zika (ZIKV), dengue (DENV), and yellow fever (YFV). Vector competence studies with Ae. aegypti often involve challenging mosquitoes with an artificial bloodmeal containing virus and later quantifying viral titer or infectious

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    Glucose Starvation, Magnesium Ion Starvation, and Bile Stress Assays
    葡萄糖饥饿、镁离子饥饿和胆汁应激分析
    作者:Aryashree Arunima and Mrutyunjay Suar日期:09/20/2021,浏览量:278,Q&A: 0
    [Abstract]

    Salmonella enterica serovar Enteritidis (S. Enteritidis) is a leading causative pathogen for food-borne gastroenteritis. During its course of infection, it confronts myriads of physiological barriers inside the host, such as nutrient deprivation, low micronutrient availability, and toxicity from bile salts, to promote bacterial survival and

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    Isolation and Characterization of Membrane Vesicles from Lactobacillus Species
    乳酸杆菌属膜囊泡中的分离和特性研究
    作者:Julie C. Caruana, Scott N. Dean and Scott A. Walper日期:09/05/2021,浏览量:784,Q&A: 0
    [Abstract]

    Throughout their life cycle, bacteria shed portions of their outermost membrane comprised of proteins, lipids, and a diversity of other biomolecules. These biological nanoparticles have been shown to have a range of highly diverse biological activities, including pathogenesis, community regulation, and cellular defense (among others). In recent

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    Plasmodium cynomolgi Berok Growth Inhibition Assay by Thiol-reactive Probe Based Flow Cytometric Measurement
    硫醇反应探针流式细胞术检测食蟹猴疟原虫生长抑制
    作者:Jessica Jie Ying Ong, Bruce Russell and Jin-Hee Han日期:09/05/2021,浏览量:646,Q&A: 0
    [Abstract]

    The relapsing malaria species, Plasmodium vivax, is the most widely distributed and difficult-to-treat cause of human malaria. The merozoites of P. vivax preferentially invade ephemeral human CD71+ reticulocytes (nascent reticulocytes), thereby limiting the development of a robust continuous culture in vitro. Fortunately, P. vivax’s sister

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    Microscopic Detection of ASC Inflammasomes in Bone Marrow Derived Macrophages Post Stimulation
    刺激后骨髓源性巨噬细胞中ASC炎症小体的显微检测
    作者:Longjun Li, Rudi Mao and Xiaopeng Qi日期:09/05/2021,浏览量:677,Q&A: 0
    [Abstract]

    An inflammasome is an intracellular multiprotein complex that plays important roles in host defense and inflammatory responses. Inflammasomes are typically composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC), cytoplasmic sensor protein, and the effector protein pro-caspase-1. ASC assembly into a protein

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    Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike
    SARS-CoV-2刺突慢病毒伪型载体制备和滴定的优化方法
    [Abstract]

    The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast and reliable protocol for the transient production of lentiviruses pseudotyped with SARS-CoV-2 Spike

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    Acid Hydrolysis for the Extraction of Archaeal Core Lipids and HPLC-MS Analysis
    酸水解法提取古生菌核心脂质及HPLC-MS分析
    作者:Maxime Tourte, Philippe Schaeffer, Vincent Grossi and Philippe M. Oger日期:08/20/2021,浏览量:389,Q&A: 0
    [Abstract]

    Lipid membranes are essential cellular elements as they provide cellular integrity and selective permeability under a broad range of environmental settings upon cell growth. In particular, Archaea are commonly recognized for their tolerance to extreme conditions, which is now widely accepted to stem from the unique structure of their lipids. While

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