微生物学

分类

    现刊
    Fluorescence-based Heme Quantitation in Toxoplasma Gondii
    基于荧光的弓形虫血红素定量
    作者:Amy Bergmann and Zhicheng Dou日期:06/20/2021,浏览量:0,Q&A: 0

    Toxoplasma gondii is a highly prevalent protozoan pathogen throughout the world. As a eukaryotic intracellular pathogen, Toxoplasma ingests nutrients from host cells to support its intracellular growth. The parasites also encode full or partial

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    Transcriptional Run-on: Measuring Nascent Transcription at Specific Genomic Sites in Yeast
    连缀转录:测定酵母特定基因组位点的新生转录
    作者:Victoria Begley, Lola de Miguel-Jiménez and Sebastián Chávez日期:06/20/2021,浏览量:0,Q&A: 0

    DNA transcription by RNA polymerases has always interested the scientific community as it is one of the most important processes involved in genome expression. This has led scientists to come up with different protocols allowing analysis of this

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    Sex-specific Separation of Plasmodium falciparum Gametocyte Populations
    恶性疟原虫配子细胞群体的性别特异性分离
    作者:Melanie C. Ridgway, Daniela Cihalova and Alexander G. Maier日期:06/05/2021,浏览量:186,Q&A: 0

    Plasmodium falciparum is a unicellular eukaryotic parasite that causes malaria in humans. The parasite is spread by Anopheles mosquitoes after ingestion of sexual stage parasites known as gametocytes. Malaria transmission depends on parasites

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    Transcriptional Run-on: Measuring Nascent Transcription at Specific Genomic Sites in Yeast
    连缀转录:测定酵母特定基因组位点的新生转录
    作者:Victoria Begley, Lola de Miguel-Jiménez and Sebastián Chávez日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    DNA transcription by RNA polymerases has always interested the scientific community as it is one of the most important processes involved in genome expression. This has led scientists to come up with different protocols allowing analysis of this process in specific locations across the genome by quantitating the amount of RNA polymerases

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    Fluorescence-based Heme Quantitation in Toxoplasma Gondii
    基于荧光的弓形虫血红素定量
    作者:Amy Bergmann and Zhicheng Dou日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Toxoplasma gondii is a highly prevalent protozoan pathogen throughout the world. As a eukaryotic intracellular pathogen, Toxoplasma ingests nutrients from host cells to support its intracellular growth. The parasites also encode full or partial metabolic pathways for the biosynthesis of certain nutrients, such as heme. Heme is an essential

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    Sex-specific Separation of Plasmodium falciparum Gametocyte Populations
    恶性疟原虫配子细胞群体的性别特异性分离
    作者:Melanie C. Ridgway, Daniela Cihalova and Alexander G. Maier日期:06/05/2021,浏览量:186,Q&A: 0
    [Abstract]

    Plasmodium falciparum is a unicellular eukaryotic parasite that causes malaria in humans. The parasite is spread by Anopheles mosquitoes after ingestion of sexual stage parasites known as gametocytes. Malaria transmission depends on parasites switching from the disease-causing asexual blood forms to male and female gametocytes. The current

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    Tracking the Subcellular Localization of Surface Proteins in Staphylococcus aureus by Immunofluorescence Microscopy
    用免疫荧光显微镜追踪金黄色葡萄球菌表面蛋白的亚细胞定位
    作者:Salvatore J. Scaffidi, Mac A. Shebes and Wenqi Yu日期:05/20/2021,浏览量:1279,Q&A: 0
    [Abstract]

    Surface proteins of Staphylococcus aureus and other Gram-positive bacteria play essential roles in bacterial colonization and host-microbe interactions. Surface protein precursors containing a YSIRK/GXXS signal peptide are translocated across the septal membrane at mid-cell, anchored to the cell wall peptidoglycan at the cross-wall compartment,

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    ODELAM: Rapid Sequence-independent Detection of Drug Resistance in Mycobacterium tuberculosis Isolates
    ODELAM:结核分枝杆菌耐药性的快速序列独立检测
    作者:Thurston Herricks, Magdalena Donczew, David R. Sherman and John D. Aitchison日期:05/20/2021,浏览量:477,Q&A: 0
    [Abstract]

    Antimicrobial-resistant Mycobacterium tuberculosis (Mtb) causes over 200,000 deaths globally each year. Current assays of antimicrobial resistance require knowledge of the mutations that confer drug resistance or long periods of culture time to test growth under drug pressure. We present ODELAM (One-cell Doubling Evaluation of Living Arrays of

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    A Fluorescence Dequenching-based Liposome Leakage Assay to Measure Membrane Permeabilization by Pore-forming Proteins
    一种以测定成孔蛋白膜通透性的基于荧光淬灭的脂质体渗漏测定方法
    作者:Javier Aguilera, Salvador Vazquez-Reyes and Jianjun Sun日期:05/20/2021,浏览量:867,Q&A: 0
    [Abstract]

    Pore-forming toxins (PFTs) have been discovered in a wide range of organisms. Their functions are essential to the survival or virulence of many species. PFTs often interact with lipid membranes. Large unilamellar vesicles (LUV), also known as liposomes, have been commonly used as reliable membrane models for testing PFTs activity. Liposomes have

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    Parasitemia Evaluation in Mice Infected with Schistosoma mansoni
    曼氏血吸虫感染小鼠的寄生虫病评估
    作者:Naiara Clemente Tavares and Marina Moraes Mourão日期:05/20/2021,浏览量:487,Q&A: 0
    [Abstract]

    Schistosomiasis is a neglected tropical disease. Its treatment relies on the use of a single drug, praziquantel. Due to treatment limitations, an alternative for schistosomiasis chemotherapy is required; thus, a better understanding of parasite biology and host-parasite interactions is valuable to aid the identification of new anti-Schistosoma

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    Inkjet 3D Printing of Polymers Resistant to Fungal Attachment
    抗真菌附着聚合物的喷墨3D打印
    作者:Yinfeng He, Cindy Vallieres, Morgan R. Alexander, Ricky D. Wildman and Simon V. Avery日期:05/05/2021,浏览量:741,Q&A: 0
    [Abstract]

    Inkjet 3D printing is an additive manufacturing method that allows the user to produce a small batch of customized devices for comparative study versus commercial products. Here, we describe the use of a commercial 2D ink development system (Dimatix material printing) to manufacture small batches of 3D medical or other devices using a recently

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    Histological Methods to Detect Early-stage Plant Defense Responses during Artificial Inoculation of Lolium perenne with Epichloë festucae
    羊茅人工接种多年生黑麦草过程中植物早期防御反应的组织学检测
    作者:Mostafa Rahnama, Damien J. Fleetwood and Richard D. Johnson日期:05/05/2021,浏览量:522,Q&A: 0
    [Abstract]

    Epichloë species form agriculturally important symbioses with many cool season grasses. To study these symbioses, such as the interaction of Epichloë festucae with perennial ryegrass (Lolium perenne), host plants can be infected by artificial inoculation of etiolated seedlings. This inoculation is performed by placing mycelium into an

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    Assessing Swarming of Aerobic Bacteria from Human Fecal Matter
    人类粪便中需氧细菌群集的评估
    作者:Arjun S. Byju, Deeti Patel, Weijie Chen and Sridhar Mani日期:05/05/2021,浏览量:457,Q&A: 0
    [Abstract]

    Swarming – swift movement across a surface via flagella propulsion – is a unique property of many bacteria. The role of swarming, particularly among bacterial populations of the human gut microbiome, is not yet fully understood; although, it is becoming an area of increased scientific and clinical inquiry. To further characterize

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