神经科学

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    现刊
    Automated Quantification of Multiple Cell Types in Fluorescently Labeled Whole Mouse Brain Sections Using QuPath
    QuPath对荧光标记的整个小鼠大脑切片中的多种细胞类型进行自动定量
    作者:Jo-Maree Courtney, Gary P. Morris, Elise M. Cleary, David W. Howells and Brad A. Sutherland日期:07/05/2022,浏览量:150,Q&A: 0

    The quantification of labeled cells in tissue sections is crucial to the advancement of biological knowledge. Traditionally, this was a tedious process, requiring hours of careful manual counting in small portions of a larger tissue section. To

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    Automated Quantification of Multiple Cell Types in Fluorescently Labeled Whole Mouse Brain Sections Using QuPath
    QuPath对荧光标记的整个小鼠大脑切片中的多种细胞类型进行自动定量
    作者:Jo-Maree Courtney, Gary P. Morris, Elise M. Cleary, David W. Howells and Brad A. Sutherland日期:07/05/2022,浏览量:150,Q&A: 0
    [Abstract]

    The quantification of labeled cells in tissue sections is crucial to the advancement of biological knowledge. Traditionally, this was a tedious process, requiring hours of careful manual counting in small portions of a larger tissue section. To overcome this, many automated methods for cell analysis have been developed. Recent advances in whole

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    Protocol to Study Spatial Subgoal Learning Using Escape Behavior in Mice
    利用逃逸行为研究小鼠空间子目标学习的方案
    作者:Philip Shamash and Tiago Branco日期:06/20/2022,浏览量:788,Q&A: 0
    [Abstract]

    Rodent spatial navigation is a key model system for studying mammalian cognition and its neural mechanisms. Of particular interest is how animals memorize the structure of their environments and compute multi-step routes to a goal. Previous work on multi-step spatial reasoning has generally involved placing rodents at the start of a maze until

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    Time-off-pick Assay to Measure Caenorhabditis elegans Motility
    时间-间隔法测定秀丽隐杆线虫运动能力
    作者:Alyssa C. Walker, Rohan Bhargava, Amanda S. Brust, Ali A. Owji and Daniel M. Czyż日期:06/20/2022,浏览量:937,Q&A: 0
    [Abstract]

    Caenorhabditis elegans is a simple metazoan that is often used as a model organism to study various human ailments with impaired motility phenotypes, including protein conformational diseases. Numerous motility assays that measure neuro-muscular function have been employed using C. elegans. Here, we describe “time-off-pick" (TOP), a novel

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    Vestibular Organ Dissection and Whole-Mount Immunolabeling in Mouse
    小鼠前庭器官解剖和整体免疫标记
    作者:Timothy S. Balmer and Laurence O. Trussell日期:05/20/2022,浏览量:435,Q&A: 0
    [Abstract]

    The vestibular sensory apparatus contained in the inner ear is a marvelous evolutionary adaptation for sensing movement in 3 dimensions and is essential for an animal’s sense of orientation in space, head movement, and balance. Damage to these systems through injury or disease can lead to vertigo, Meniere’s disease, and other disorders

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    Identification and Monitoring of Nucleotide Repeat Expansions Using Southern Blotting in Drosophila Models of C9orf72 Motor Neuron Disease and Frontotemporal Dementia
    应用Southern Blotting鉴定和监测C9orf72运动神经元疾病和额颞叶痴呆模型的核苷酸重复扩增
    作者:Joanne L. Sharpe, Nikki S. Harper and Ryan J. H. West日期:05/20/2022,浏览量:423,Q&A: 0
    [Abstract]

    Repeat expansion diseases, including fragile X syndrome, Huntington’s disease, and C9orf72-related motor neuron disease and frontotemporal dementia, are a group of disorders associated with polymorphic expansions of tandem repeat nucleotide sequences. These expansions are highly repetitive and often hundreds to thousands of repeats in

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    Translating Ribosome Affinity Purification (TRAP) of Cell Type-specific mRNA from Mouse Brain Lysates
    小鼠脑裂解物中细胞类型特异性 mRNA 的核糖体翻译亲和纯化 (TRAP)
    作者:Catherine L. Salussolia, Kellen D. Winden and Mustafa Sahin日期:05/05/2022,浏览量:629,Q&A: 0
    [Abstract]

    Mammalian tissues are highly heterogenous and complex, posing a challenge in understanding the molecular mechanisms regulating protein expression within various tissues. Recent studies have shown that translation at the level of the ribosome is highly regulated, and can vary independently of gene expression observed at a transcriptome level, as

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    High Throughput Blood-brain Barrier Organoid Generation and Assessment of Receptor-Mediated Antibody Transcytosis
    高通量血脑屏障类器官的产生和受体介导的抗体转胞吞作用的评估
    作者:Elena Kassianidou, Claire Simonneau, Alina Gavrilov and Roberto Villaseñor日期:04/20/2022,浏览量:675,Q&A: 0
    [Abstract]

    Targeting receptor-mediated transcytosis (RMT) is a successful strategy for drug delivery of biologic agents across the blood-brain barrier (BBB). The recent development of human BBB organoid models is a major advancement to help characterize the mechanisms of RMT and thus accelerate the design of brain delivery technologies. BBB organoids exhibit

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    Multiplexing Thermotaxis Behavior Measurement in Caenorhabditis elegans
    秀丽隐杆线虫的多重趋热行为测量
    作者:Stephan Raiders, Mason Klein and Aakanksha Singhvi日期:04/05/2022,浏览量:857,Q&A: 0
    [Abstract]

    Thermotaxis behaviors in C. elegans exhibit experience-dependent plasticity of thermal preference memory. This behavior can be assayed either at population level, on linear temperature gradients, or at the individual animal level, by radial isothermal or microfluidic tracking of orientation. These behaviors are low-throughput as well as

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    Stimulus-induced Robust Narrow-band Gamma Oscillations in Human EEG Using Cartesian Gratings
    使用笛卡尔光栅在人类脑电图中刺激诱导的稳健窄带伽马振荡
    作者:Dinavahi V. P. S. Murty and Supratim Ray日期:04/05/2022,浏览量:460,Q&A: 0
    [Abstract]

    Stimulus-induced narrow-band gamma oscillations (20–70 Hz) are induced in the visual areas of the brain when particular visual stimuli, such as bars, gratings, or full-screen hue, are shown to the subject. Such oscillations are modulated by higher cognitive functions, like attention, and working memory, and have been shown to be abnormal in

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    Optogenetic Targeting of Mouse Vagal Afferents Using an Organ-specific, Scalable, Wireless Optoelectronic Device
    使用器官特异性、可扩展的无线光电设备对小鼠迷走神经传入进行光遗传学靶向研究
    作者:Sungcheol Hong, Woo Seok Kim, Yong Han, Rahul Cherukuri, Haemin Jung, Carlos Campos, Qi Wu and Sung Il Park日期:03/05/2022,浏览量:885,Q&A: 0
    [Abstract]

    Optogenetics has the potential to transform the study of the peripheral nervous system (PNS), but the complex anatomy of the PNS poses unique challenges for the focused delivery of light to specific tissues. This protocol describes the fabrication of a wireless telemetry system for studying peripheral sensory pathways. Unlike existing wireless

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