干细胞

分类

    现刊
    Assessing the Presence of Hematopoietic Stem and Progenitor Cells in Mouse Spleen
    评估小鼠脾脏中造血干细胞和祖细胞的存在
    作者:Isabelle J. Marié, Lara Brambilla and David E. Levy日期:06/05/2022,浏览量:654,Q&A: 0
    [Abstract]

    Transplantation of hematopoietic material into recipient mice is an assay routinely used to determine the presence and function of hematopoietic stem and progenitor cells (HSPCs) in vivo. The principle of the method is to transplant donor cells being tested for HSPCs into a recipient mouse following bone marrow ablation and testing for

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    Click-iT® Plus OPP Alexa Fluor® Protein Synthesis Assay in Embryonic Cells
    在胚胎细胞中Click- iT®Plus OPP Alexa Fluor®蛋白合成分析
    作者:Yan Li, Xu Ji, Lu Chang, Jianan Tang, Min-Min Hua, Jing Liu, Christopher O’Neil, Xuefeng Huang and Xingliang Jin日期:06/05/2022,浏览量:611,Q&A: 0
    [Abstract]

    This protocol describes a method to assess relative changes in the level of global protein synthesis in the preimplantation embryo using the Click-iT® Plus OPP Protein Synthesis Assays. In this assay, O-propargyl-puromycin (OPP), an analog of puromycin, is efficiently incorporated into the nascent polypeptide of newly translated proteins in

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    Optimized CRISPR-Cas9-based Strategy for Complex Gene Targeting in Murine Embryonic Stem Cells for Germline Transmission
    用于生殖系传递的基于 CRISPR-Cas9 的小鼠胚胎干细胞中优化的复杂基因靶向策略
    作者:Thomas J. O'Neill, Daniel Krappmann and Andreas Gewies日期:05/20/2022,浏览量:823,Q&A: 0
    [Abstract]

    Although CRISPR-Cas9 genome editing can be performed directly in single-cell mouse zygotes, the targeting efficiency for more complex modifications such as the insertion of two loxP sites, multiple mutations in cis, or the precise insertion or deletion of longer DNA sequences often remains low (Cohen, 2016). Thus, targeting and validation of

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    Isolation and Culture of Cranial Neural Crest Cells from the First Branchial Arch of Mice
    小鼠第一鳃弓颅神经嵴细胞的分离培养
    作者:Hiroki Ueharu, Jingwen Yang, Yoshihiro Komatsu and Yuji Mishina日期:04/05/2022,浏览量:633,Q&A: 0
    [Abstract]

    Craniofacial anomalies (CFA) are a diverse group of deformities, which affect the growth of the head and face. Dysregulation of cranial neural crest cell (NCC) migration, proliferation, differentiation, and/or cell fate specification have been reported to contribute to CFA. Understanding of the mechanisms through which cranial NCCs contribute

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    Differentiation of Human Induced Pluripotent Stem Cell into Macrophages
    人诱导多能干细胞向巨噬细胞的分化
    作者:Harriet Douthwaite, Aitor Bermejo Arteagabeitia and Subhankar Mukhopadhyay日期:03/20/2022,浏览量:1058,Q&A: 0
    [Abstract]

    As a model to interrogate human macrophage biology, macrophages differentiated from human induced pluripotent stem cells (hiPSCs) transcend other existing models by circumventing the variability seen in human monocyte-derived macrophages, whilst epitomizing macrophage phenotypic and functional characteristics over those offered by macrophage-like

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    Skeletal Stem Cell Isolation from Cranial Suture Mesenchyme and Maintenance of Stemness in Culture
    从颅缝间充质中分离骨骼干细胞和培养干细胞的维持
    作者:Takamitsu Maruyama, Hsiao-Man Ivy Yu and Wei Hsu日期:03/05/2022,浏览量:793,Q&A: 0
    [Abstract]

    Skeletal stem cells residing in the suture mesenchyme are responsible for calvarial development, homeostatic maintenance, and injury-induced repair. These naïve cells exhibit long-term self-renewal, clonal expansion, and multipotency. They possess osteogenic abilities to regenerate bones in a cell-autonomous manner and can directly replace

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    Efficient Method to Differentiate Mouse Embryonic Stem Cells into Macrophages in vitro
    将小鼠胚胎干细胞体外分化为巨噬细胞的有效方法
    作者:Qimin Hai, Juying Han, Sophia Wells and Jonathan D. Smith日期:02/05/2022,浏览量:1492,Q&A: 0
    [Abstract]

    Macrophages are key cells in the innate immune system and play a role in a variety of diseases. However, macrophages are terminally differentiated and difficult to manipulate genetically via transfection or through CRISPR-Cas9 gene editing. To overcome this limitation, we provide a simplified protocol for the generation of mouse embryonic stem

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    Flow Cytometry Analysis of Planarian Stem Cells Using DNA and Mitochondrial Dyes
    利用DNA和线粒体染料流式细胞术分析涡虫干细胞
    作者:Mohamed Mohamed Haroon, Praveen Kumar Vemula and Dasaradhi Palakodeti日期:01/20/2022,浏览量:1127,Q&A: 0
    [Abstract]

    Planarians are free-living flatworms that emerged as a crucial model system to understand regeneration and stem cell biology. The ability to purify neoblasts, the adult stem cell population of planaria, through fluorescence-activated cell sorting (FACS) has tremendously increased our understanding of pluripotency, specialization, and

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    From 3D to 2D: Harmonization of Protocols for Two-dimensional Cultures on Cell Culture Inserts of Intestinal Organoids from Various Species
    从三维到二维:不同物种肠道类器官细胞培养插件的二维培养方法的协调
    [Abstract]

    In the expanding field of intestinal organoid research, various protocols for three- and two-dimensional organoid-derived cell cultures exist. Two-dimensional organoid-derived monolayers are used to overcome some limitations of three-dimensional organoid cultures. They are increasingly used also in infection research, to study physiological

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    An in vitro Mechanical Damage Model of Isolated Myofibers in a Floating Culture Condition
    漂浮培养条件下离体肌纤维的体外机械损伤模型
    作者:Yoshifumi Tsuchiya and Yusuke Ono日期:01/05/2022,浏览量:1161,Q&A: 0
    [Abstract]

    Muscle stem cells (satellite cells), located on the surface of myofibers, are rapidly activated from a quiescent state following skeletal muscle injury. Although satellite cell activation is an initial step in muscle regeneration, the stimulation of satellite cell activation by muscle injury remains to be elucidated. We recently established an in

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