系统生物学

分类

    现刊
    Isolation of Nuclei from Mouse Dorsal Root Ganglia for Single-nucleus Genomics
    小鼠背根神经节细胞核的分离及其单核基因组学研究
    作者:Lite Yang, Ivan Tochitsky, Clifford J. Woolf and William Renthal日期:08/05/2021,浏览量:669,Q&A: 0
    [Abstract]

    Primary somatosensory neurons, whose cell bodies reside in the dorsal root ganglion (DRG) and trigeminal ganglion, are specialized to transmit sensory information from the periphery to the central nervous system. Our molecular understanding of peripheral sensory neurons has been limited by both their heterogeneity and low abundance compared with

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    Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease
    用于研究人类疾病中蛋白质N-糖基化失调的N-糖肽丰度和N-糖基化位点的差异分析
    作者:Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin日期:06/20/2021,浏览量:1057,Q&A: 0
    [Abstract]

    Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass spectrometry-based glycoproteomics technologies enabling large-scale N-glycoproteome profiling of

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    Detecting Differentially Methylated Promoters in Genes Related to Disease Phenotypes Using R
    利用R检测与疾病表型相关基因的差异甲基化启动子
    作者:Jordi Martorell Marugán and Pedro Carmona-Sáez日期:06/05/2021,浏览量:1209,Q&A: 0
    [Abstract]

    DNA methylation in gene promoters plays a major role in gene expression regulation, and alterations in methylation patterns have been associated with several diseases. In this context, different software suites and statistical methods have been proposed to analyze differentially methylated positions and regions. Among them, the novel statistical

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    Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
    基于抗体系标记的简化表观基因组图谱
    作者:Steven Henikoff, Jorja G. Henikoff and Kami Ahmad日期:06/05/2021,浏览量:1623,Q&A: 0
    [Abstract]

    We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples and single cells. With CUT&Tag, intact cells or nuclei are permeabilized, followed by successive

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    Identification of R-loop-forming Sequences in Drosophila melanogaster Embryos and Tissue Culture Cells Using DRIP-seq
    DRIP-seq法鉴定黑腹果蝇胚胎和组织培养细胞R-loop形成序列
    作者:Célia Alecki and Nicole J. Francis日期:05/05/2021,浏览量:2943,Q&A: 0
    [Abstract]

    R-loops are non-canonical nucleic structures composed of an RNA–DNA hybrid and a displaced ssDNA. Originally identified as a source of genomic instability, R-loops have been shown over the last decade to be involved in the targeting of proteins and to be associated with different histone modifications, suggesting a regulatory function. In

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    Computational Analysis and Phylogenetic Clustering of SARS-CoV-2 Genomes
    SARS-CoV-2基因组计算分析及系统进化聚类分析
    作者:Bani Jolly and Vinod Scaria日期:04/20/2021,浏览量:2693,Q&A: 0
    [Abstract]

    COVID-19, the disease caused by the novel SARS-CoV-2 coronavirus, originated as an isolated outbreak in the Hubei province of China but soon created a global pandemic and is now a major threat to healthcare systems worldwide. Following the rapid human-to-human transmission of the infection, institutes around the world have made efforts to generate

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    Trypanosomatid, fluorescence-based in vitro U-insertion/U-deletion RNA-editing (FIDE)
    基于荧光的锥虫体体外尿嘧啶插入或删除RNA编辑(FIDE)
    作者:Wolf-Matthias Leeder, Elisabeth Kruse and H. Ulrich Göringer日期:03/05/2021,浏览量:1787,Q&A: 0
    [Abstract]

    Gene expression within the mitochondria of African trypanosomes and other protozoan organisms relies on a nucleotide-specific RNA-editing reaction. In the process exclusively uridine (U)-nucleotides are site-specifically inserted into and deleted from sequence-deficient primary transcripts to convert them into translatable mRNAs. The reaction is

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    Primer ID Next-Generation Sequencing for the Analysis of a Broad Spectrum Antiviral Induced Transition Mutations and Errors Rates in a Coronavirus Genome
    用于分析冠状病毒基因组中广谱抗病毒药物诱导的过渡突变和错误率的引物ID下一代测序
    作者:Shuntai Zhou, Collin S. Hill, Michael U. Clark, Timothy P. Sheahan, Ralph Baric and Ronald Swanstrom日期:03/05/2021,浏览量:3285,Q&A: 0
    [Abstract]

    Next generations sequencing (NGS) has become an important tool in biomedical research. The Primer ID approach combined with the MiSeq platform overcomes the limitation of PCR errors and reveals the true sampling depth of population sequencing, making it an ideal tool to study mutagenic effects of potential broad-spectrum antivirals on RNA viruses.

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    EmPC-seq: Accurate RNA-sequencing and Bioinformatics Platform to Map RNA Polymerases and Remove Background Error
    EmPC-seq: 使用精确的RNA测序和生物信息学平台绘制RNA聚合酶并消除背景错误
    [Abstract]

    Transcription errors can substantially affect metabolic processes in organisms by altering the epigenome and causing misincorporations in mRNA, which is translated into aberrant mutant proteins. Moreover, within eukaryotic genomes there are specific Transcription Error-Enriched genomic Loci (TEELs) which are transcribed by RNA polymerases with

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    Low- and High-resolution Dynamic Analyses for Magnetic Resonance Spectroscopy Data
    磁共振光谱数据的低高分辨率动态分析
    作者:Reuben Rideaux日期:01/20/2021,浏览量:1450,Q&A: 0
    [Abstract]

    Magnetic resonance spectroscopy (MRS) can be used to measure in vivo concentrations of neurometabolites. This information can be used to identify neurotransmitter involvement in healthy (e.g., perceptual and cognitive processes) and unhealthy brain function (e.g., neurological and psychiatric illnesses). The standard approach for analyzing MRS

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