癌症生物学

分类

    现刊
    Analysis of Leukemia Cell Metabolism through Stable Isotope Tracing in Mice
    小鼠白血病细胞代谢的稳定同位素示踪分析
    作者:Nick van Gastel, Jessica B. Spinelli, Marcia C. Haigis and David T. Scadden日期:10/05/2021,浏览量:753,Q&A: 0

    Once thought to be a mere consequence of the state of a cell, intermediary metabolism is now recognized as a key regulator of mammalian cell fate and function. In addition, cell metabolism is often disturbed in malignancies such as cancer, and

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    Analysis of Leukemia Cell Metabolism through Stable Isotope Tracing in Mice
    小鼠白血病细胞代谢的稳定同位素示踪分析
    作者:Nick van Gastel, Jessica B. Spinelli, Marcia C. Haigis and David T. Scadden日期:10/05/2021,浏览量:753,Q&A: 0
    [Abstract]

    Once thought to be a mere consequence of the state of a cell, intermediary metabolism is now recognized as a key regulator of mammalian cell fate and function. In addition, cell metabolism is often disturbed in malignancies such as cancer, and targeting metabolic pathways can provide new therapeutic options. Cell metabolism is mostly studied in

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    Comprehensive Identification of Translatable Circular RNAs Using Polysome Profiling
    利用多聚体谱综合鉴定可翻译环状RNA
    作者:Yanwen Ye, Zefeng Wang and Yun Yang日期:09/20/2021,浏览量:741,Q&A: 0
    [Abstract]

    Circular RNAs (circRNAs), a special type of RNAs without 5’- and 3’-ends, are widely present in eukaryotes and known to function as noncoding RNAs to regulate gene expression, including as miRNA sponges. Recent studies showed that many exonic circRNAs, generated by back-splicing of pre-mRNAs, can be translated in a cap-independent

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    Neutral Comet Assay to Detect and Quantitate DNA Double-Strand Breaks in Hematopoietic Stem Cells
    检测和定量造血干细胞DNA双链断裂的中性彗星试验
    作者:Irene Mariam Roy, Pon Sowbhagya Nadar and Satish Khurana日期:08/20/2021,浏览量:622,Q&A: 0
    [Abstract]

    In vertebrates, hematopoietic stem cells (HSCs) regulate the supply of blood cells throughout the lifetime and help to maintain homeostasis. Due to their long lifespan, genetic integrity is paramount for these cells, and accordingly, a number of stem cell-specific mechanisms are employed. However, HSCs tend to show more DNA damage with increasing

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    Measuring DNA Damage Using the Alkaline Comet Assay in Cultured Cells
    培养细胞DNA损伤的碱性彗星试验
    作者:Elena Clementi, Zuzana Garajova and Enni Markkanen日期:08/20/2021,浏览量:1082,Q&A: 0
    [Abstract]

    Maintenance of DNA integrity is of pivotal importance for cells to circumvent detrimental processes that can ultimately lead to the development of various diseases. In the face of a plethora of endogenous and exogenous DNA-damaging agents, cells have evolved a variety of DNA repair mechanisms that are responsible for safeguarding genetic

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    A Genetically Engineered Mouse Model of Venous Anomaly and Retinal Angioma-like Vascular Malformation
    静脉畸形和视网膜血管瘤样血管畸形的基因工程小鼠模型
    作者:Xudong Cao, Beibei Xu, Xiao Li, Taotao Li and Yulong He日期:08/05/2021,浏览量:870,Q&A: 0
    [Abstract]

    Characterization of key regulators in vein development will advance our understanding of mechanisms underlying venous anomalies and provide therapeutic targets for the treatment of vascular malformations. Here, we provide a detailed protocol for the generation of genetically engineered mouse models targeting the Tek gene for the analysis of vein

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    Fe-NTA Microcolumn Purification of Phosphopeptides from Immunoprecipitation (IP) Eluates for Mass Spectrometry Analysis
    Fe-NTA微柱纯化免疫沉淀(IP)洗脱物中磷酸肽的质谱分析
    作者:Ethan J. Sanford and Marcus B. Smolka日期:08/05/2021,浏览量:591,Q&A: 0
    [Abstract]

    Protein phosphorylation is a nearly universal signaling mechanism. To date, a number of proteomics tools have been developed to analyze phosphorylation. Phosphoproteome-wide analyses using whole cell extracts suffer from incomplete coverage, often missing phosphorylation events from low-abundance proteins. In order to increase coverage of

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    Modeling the Nonlinear Dynamics of Intracellular Signaling Networks
    细胞内信号网络的非线性动力学建模
    作者:Oleksii S. Rukhlenko and Boris N. Kholodenko日期:07/20/2021,浏览量:1301,Q&A: 0
    [Abstract]

    This protocol illustrates a pipeline for modeling the nonlinear behavior of intracellular signaling pathways. At fixed spatial points, nonlinear signaling dynamics are described by ordinary differential equations (ODEs). At constant parameters, these ODEs may have multiple attractors, such as multiple steady states or limit cycles. Standard

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    Antisense Oligo Pulldown of Circular RNA for Downstream Analysis
    用于下游分析的环状RNA的反义Oligo Pulldown
    作者:Debojyoti Das, Aniruddha Das and Amaresh C. Panda日期:07/20/2021,浏览量:854,Q&A: 0
    [Abstract]

    Circular RNAs (circRNAs) are a large family of noncoding RNA molecules that have emerged as novel regulators of gene expression by sequestering microRNAs (miRNAs) and RNA-binding proteins (RBPs). Several computational tools have been developed to predict circRNA interaction with target miRNAs and RBPs with a view to studying their potential effect

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    Analysis of the Effects of Hexokinase 2 Detachment From Mitochondria-Associated Membranes with the Highly Selective Peptide HK2pep
    用高选择性肽HK2pep分析己糖激酶2脱离线粒体相关膜的作用
    作者:Francesco Ciscato, Federica Chiara, Riccardo Filadi and Andrea Rasola日期:07/20/2021,浏览量:1402,Q&A: 0
    [Abstract]

    The crucial role of hexokinase 2 (HK2) in the metabolic rewiring of tumors is now well established, which makes it a suitable target for the design of novel therapies. However, hexokinase activity is central to glucose utilization in all tissues; thus, enzymatic inhibition of HK2 can induce severe adverse effects. In an effort to find a selective

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    Monitoring Changes in the Oxidizing Milieu in the Endoplasmic Reticulum of Mammalian Cells Using HyPerER
    利用HyPerER监测哺乳动物细胞内质网氧化环境的变化
    作者:Julia Birk, Beata Lizak, Christian Appenzeller-Herzog and Alex Odermatt日期:07/05/2021,浏览量:1533,Q&A: 0
    [Abstract]

    The production of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress are tightly linked. The generation of ROS can be both the cause and a consequence of ER stress pathways, and an increasing number of human diseases are characterized by tissue atrophy in response to ER stress and oxidative injury. For the assessment of modulators

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