发育生物学

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    现刊
    GeneWeld: Efficient Targeted Integration Directed by Short Homology in Zebrafish
    GeneWeld:斑马鱼短同源性诱导的高效靶向整合

    Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Multiple design strategies for zebrafish gene targeting have previously been reported with widely varying frequencies for

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    GeneWeld: Efficient Targeted Integration Directed by Short Homology in Zebrafish
    GeneWeld:斑马鱼短同源性诱导的高效靶向整合
    [Abstract]

    Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Multiple design strategies for zebrafish gene targeting have previously been reported with widely varying frequencies for germline recovery of integration alleles. The GeneWeld protocol and pGTag (plasmids for Gene Tagging)

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    An Improved Method for Individual Tracking of Voluntary Wheel Running in Pair-housed Juvenile Mice
    一种对饲养的幼鼠自主跑轮运动个体跟踪的改进方法
    作者:David A. Valientes, Anthony M. Raus and Autumn S. lvy日期:07/05/2021,浏览量:681,Q&A: 0
    [Abstract]

    Rodent cages equipped with access to a voluntary running wheel are commonly used to study the effects of aerobic physical activity on physiology and behavior. Notable discoveries in exercise neurobiology, including the key role of brain-derived neurotrophic factor (BDNF) in neural plasticity and cognition, have been made using rodents housed with

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    Analysis of Caenorhabditis elegans Sperm Number, Size, Activation, and Mitochondrial Content
    秀丽隐杆线虫精子数量、大小、活化及线粒体含量分析
    作者:Amy M. Hammerquist, Chia-An Yen and Sean P. Curran日期:06/05/2021,浏览量:1186,Q&A: 0
    [Abstract]

    Infertility is a widespread and often unexplained issue. Studying reproduction using C. elegans males offers insight into the influence of individual factors on male fertility in humans. We have created a collection of protocols to assess several aspects of C. elegans sperm quality, including number, size, rate of activation, and mitochondrial

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    Rice Root Hair Phenotypes Imaged by Cryo-SEM
    水稻根毛表型的低温扫描电镜成像
    [Abstract]

    Cryo-scanning electron microscopy (cryo-SEM) was first introduced for scientific use in the 1980s. Since then, cryo-SEM has become a routine technique for studying the surfaces and internal structures of biological samples with a high water content. In contrast to traditional SEM, cryo-SEM requires no sample pretreatment processes; thus, we can

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    Quantitation of Secretory Granule Size in Drosophila Larval Salivary Glands
    果蝇幼虫唾液腺分泌颗粒大小的定量研究
    作者:Cheng-I J. Ma and Julie A. Brill日期:06/05/2021,浏览量:1141,Q&A: 0
    [Abstract]

    Maturation of secretory granules is a crucial process that ensures the bioactivity of cargo proteins undergoing regulated secretion. In Drosophila melanogaster, the larval salivary glands produce secretory granules that are up to four-fold larger in cross-sectional area after maturation. Therefore, we developed a live imaging microscopy approach

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    Visualization and Quantitation of Wg trafficking in the Drosophila Wing Imaginal Epithelium
    果蝇翅成虫上皮中Wg运输的可视化和定量研究
    作者:Leonie Witte, Karen Linnemannstöns, Mona Honemann-Capito and Julia Christina Gross日期:06/05/2021,浏览量:1185,Q&A: 0
    [Abstract]

    Secretory Wnt trafficking can be studied in the polarized epithelial monolayer of Drosophila wing imaginal discs (WID). In this tissue, Wg (Drosophila Wnt-I) is presented on the apical surface of its source cells before being internalized into the endosomal pathway. Long-range Wg secretion and spread depend on secondary secretion from endosomal

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    Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis
    小鼠多能干细胞衍生干细胞样结构的生成:植入术后胚胎发生的体外模型
    [Abstract]

    Post-implantation mammalian embryogenesis involves profound molecular, cellular, and morphogenetic changes. The study of these highly dynamic processes is complicated by the limited accessibility of in utero development. In recent years, several complementary in vitro systems comprising self-organized assemblies of mouse embryonic stem cells, such

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    Isolation of First-Trimester and Full-term Human Placental Hofbauer Cells
    妊娠早期和足月人胎盘霍夫鲍尔细胞的分离
    作者:Anna Appios, Jake R. Thomas and Naomi McGovern日期:06/05/2021,浏览量:1551,Q&A: 0
    [Abstract]

    The placenta is the crucial organ that regulates the health of both mother and fetus during pregnancy. The human placenta is composed of villous tree-like structures that embed into the maternal decidua. Within the stroma of the villi resides a population of fetally-derived macrophages, the Hofbauer cells (HBC). HBC are the only fetal immune cells

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    Imaging and Fluorescence Quantification in Caenorhabditis elegans with Flow Vermimetry and Automated Microscopy
    秀丽隐杆线虫的流动蠕虫仪和自动显微镜成像和荧光定量研究
    作者:Elissa Tjahjono, Alexey V. Revtovich and Natalia V. Kirienko日期:05/20/2021,浏览量:1639,Q&A: 0
    [Abstract]

    Gene activation and cellular biomarkers are commonly monitored using fluorescent signals from transgenic reporters or dyes. These quantifiable markers are critical for biological research and serve as an incredibly powerful tool, even more so when combined with high-throughput screening. Caenorhabditis elegans is a particularly useful model in

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    Analysis of Monocyte Cell Fate by Adoptive Transfer in a Murine Model of TLR7-induced Systemic Inflammation
    TLR7诱导的系统性炎症小鼠模型中通过过继转移单核细胞命运分析
    作者:Jaba Gamrekelashvili, Hermann Haller and Florian P. Limbourg日期:05/05/2021,浏览量:2489,Q&A: 0
    [Abstract]

    Myeloid plasticity is a hallmark of the innate immune response to Toll-like receptor (TLR) activation. Here, we provide a protocol for monocyte cell fate tracking by adoptive transfer in the context of systemic inflammation induced by TLR7 activation, the principal innate immune receptor sensing viral RNA in mice. Defined monocyte subsets are

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