生物化学

分类

    现刊
    Proteomics Profiling of S-sulfurated Proteins in Acinetobacter baumannii
    鲍曼不动杆菌硫酸化蛋白的蛋白质组学分析
    作者:Brenna J. C. Walsh and David P. Giedroc日期:05/05/2021,浏览量:197,Q&A: 0

    Hydrogen sulfide (H2S) is emerging as an important modulator in bacterial cytoprotection against the host immune response in infected animals, which may well be attributed to downstream highly oxidized sulfur species, termed reactive sulfur species

    ...
    Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum
    恶性疟原虫无性期原位蛋白质棕榈酰化的点击化学成像
    作者:Mansoor A. Siddiqui日期:05/05/2021,浏览量:339,Q&A: 0

    Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases

    ...
    Spin Labeling of RNA Using “Click” Chemistry for Coarse-grained Structure Determination via Pulsed Electron-electron Double Resonance Spectroscopy
    利用脉冲电子-电子双共振光谱测定粗粒结构的“点击”化学的RNA自旋标记
    作者:Maria F. Vicino, Tobias Hett and Olav Schiemann日期:05/05/2021,浏览量:201,Q&A: 0

    Understanding the function of oligonucleotides on a molecular level requires methods for studying their structure, conformational changes, and internal dynamics. Various biophysical methods exist to achieve this, including the whole toolbox of

    ...
    COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR
    COVID-19样本汇集:从RNA提取到实时定量RT-PCR

    The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and

    ...
    Histological Methods to Detect Early-stage Plant Defense Responses during Artificial Inoculation of Lolium perenne with Epichloë festucae
    羊茅人工接种多年生黑麦草过程中植物早期防御反应的组织学检测
    作者:Mostafa Rahnama, Damien J. Fleetwood and Richard D. Johnson日期:05/05/2021,浏览量:162,Q&A: 0

    Epichloë species form agriculturally important symbioses with many cool season grasses. To study these symbioses, such as the interaction of Epichloë festucae with perennial ryegrass (Lolium perenne), host plants can be infected by

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    Chromatographic Assays for the Enzymatic Degradation of Chitin
    几丁质酶解的色谱分析
    作者:Sophanit Mekasha, Tina R. Tuveng, Gustav Vaaje-Kolstad and Vincent G. H. Eijsink日期:05/05/2021,浏览量:230,Q&A: 0

    Chitin is an insoluble linear polymer of β(1→4)-linked N-acetylglucosamine. Enzymatic cleavage of chitin chains can be achieved using hydrolytic enzymes, called chitinases, and/or oxidative enzymes, called lytic polysaccharide

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    Characterising Plant Deubiquitinases with in vitro Activity-based Labelling and Ubiquitin Chain Disassembly Assays
    植物去泛素酶的体外活性标记和泛素链分解分析
    作者:Michael J. Skelly and Steven H. Spoel日期:05/05/2021,浏览量:224,Q&A: 0

    Post-translational modification of proteins by ubiquitin is an essential cellular signaling mechanism in all eukaryotes. Ubiquitin is removed from target proteins by a wide range of deubiquitinase (DUB) enzymes with different activities and

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    Characterising Plant Deubiquitinases with in vitro Activity-based Labelling and Ubiquitin Chain Disassembly Assays
    植物去泛素酶的体外活性标记和泛素链分解分析
    作者:Michael J. Skelly and Steven H. Spoel日期:05/05/2021,浏览量:224,Q&A: 0
    [Abstract]

    Post-translational modification of proteins by ubiquitin is an essential cellular signaling mechanism in all eukaryotes. Ubiquitin is removed from target proteins by a wide range of deubiquitinase (DUB) enzymes with different activities and substrate specificities. Understanding how DUBs function in vitro is a vital first step to uncovering their

    ...
    Chromatographic Assays for the Enzymatic Degradation of Chitin
    几丁质酶解的色谱分析
    作者:Sophanit Mekasha, Tina R. Tuveng, Gustav Vaaje-Kolstad and Vincent G. H. Eijsink日期:05/05/2021,浏览量:230,Q&A: 0
    [Abstract]

    Chitin is an insoluble linear polymer of β(1→4)-linked N-acetylglucosamine. Enzymatic cleavage of chitin chains can be achieved using hydrolytic enzymes, called chitinases, and/or oxidative enzymes, called lytic polysaccharide monooxygenases (LPMOs). These two groups of enzymes have different modes of action and yield different product

    ...
    Histological Methods to Detect Early-stage Plant Defense Responses during Artificial Inoculation of Lolium perenne with Epichloë festucae
    羊茅人工接种多年生黑麦草过程中植物早期防御反应的组织学检测
    作者:Mostafa Rahnama, Damien J. Fleetwood and Richard D. Johnson日期:05/05/2021,浏览量:162,Q&A: 0
    [Abstract]

    Epichloë species form agriculturally important symbioses with many cool season grasses. To study these symbioses, such as the interaction of Epichloë festucae with perennial ryegrass (Lolium perenne), host plants can be infected by artificial inoculation of etiolated seedlings. This inoculation is performed by placing mycelium into an

    ...
    COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR
    COVID-19样本汇集:从RNA提取到实时定量RT-PCR
    [Abstract]

    The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume

    ...
    Spin Labeling of RNA Using “Click” Chemistry for Coarse-grained Structure Determination via Pulsed Electron-electron Double Resonance Spectroscopy
    利用脉冲电子-电子双共振光谱测定粗粒结构的“点击”化学的RNA自旋标记
    作者:Maria F. Vicino, Tobias Hett and Olav Schiemann日期:05/05/2021,浏览量:201,Q&A: 0
    [Abstract]

    Understanding the function of oligonucleotides on a molecular level requires methods for studying their structure, conformational changes, and internal dynamics. Various biophysical methods exist to achieve this, including the whole toolbox of Electron Paramagnetic Resonance (EPR or ESR) spectroscopy. An EPR method widely used in this regard is

    ...
    Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum
    恶性疟原虫无性期原位蛋白质棕榈酰化的点击化学成像
    作者:Mansoor A. Siddiqui日期:05/05/2021,浏览量:339,Q&A: 0
    [Abstract]

    Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases (PATs or DHHC-PATs). Palmitoylation has recently emerged as a crucial post-translational

    ...
    Proteomics Profiling of S-sulfurated Proteins in Acinetobacter baumannii
    鲍曼不动杆菌硫酸化蛋白的蛋白质组学分析
    作者:Brenna J. C. Walsh and David P. Giedroc日期:05/05/2021,浏览量:197,Q&A: 0
    [Abstract]

    Hydrogen sulfide (H2S) is emerging as an important modulator in bacterial cytoprotection against the host immune response in infected animals, which may well be attributed to downstream highly oxidized sulfur species, termed reactive sulfur species (RSS), derived from H2S. One mechanism by which H2S/RSS may signal in the cell is through proteome

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    Optimized Recombinant Production of Secreted Proteins Using Human Embryonic Kidney (HEK293) Cells Grown in Suspension
    利用悬浮培养的人胚胎肾(HEK293)细胞优化分泌蛋白的重组生产
    [Abstract]

    Recombinant proteins are an essential milestone for a plethora of different applications ranging from pharmaceutical to clinical, and mammalian cell lines are among the currently preferred systems to obtain large amounts of proteins of interest due to their high level of post-translational modification and manageable large-scale production. In

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    Quantitative Characterization of the Amount and Length of (1,3)-β-D-glucan for Functional and Mechanistic Analysis of Fungal (1,3)-β-D-glucan Synthase
    通过(1,3)-β-d-葡聚糖量和长度的定量表征对真菌(1,3)-β-d-葡聚糖合酶进行功能和机理分析
    作者:Abhishek Chhetri, Anna Loksztejn and Kenichi Yokoyama日期:04/20/2021,浏览量:801,Q&A: 0
    [Abstract]

    (1,3)-β-d-Glucan synthase (GS) is an essential enzyme for fungal cell wall biosynthesis that catalyzes the synthesis of (1,3)-β-d-glucan, a major and vital component of the cell wall. GS is a proven target of antifungal antibiotics including FDA-approved echinocandin derivatives; however, the function and mechanism of GS remain largely

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    Reconstitution of Chromatin by Stepwise Salt Dialysis
    逐步盐透析重组染色质
    作者:Grisel Cruz-Becerra and James T. Kadonaga日期:04/05/2021,浏览量:312,Q&A: 0
    [Abstract]

    Chromatin, rather than plain DNA, is the natural substrate of the molecular machines that mediate DNA-directed processes in the nucleus. Chromatin can be reconstituted in vitro by using different methodologies. The salt dialysis method yields chromatin that consists of purified histones and DNA. This biochemically pure chromatin is well-suited for

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