生物化学

分类

    现刊
    Comprehensive Identification of Translatable Circular RNAs Using Polysome Profiling
    利用多聚体谱综合鉴定可翻译环状RNA
    作者:Yanwen Ye, Zefeng Wang and Yun Yang日期:09/20/2021,浏览量:585,Q&A: 0

    Circular RNAs (circRNAs), a special type of RNAs without 5’- and 3’-ends, are widely present in eukaryotes and known to function as noncoding RNAs to regulate gene expression, including as miRNA sponges. Recent studies showed that many

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    Anaerobic Expression and Purification of Holo-CCIS, an Artificial Iron-sulfur Protein
    人工铁硫蛋白 Holo-CCIS的厌氧表达与纯化
    作者:Bhanu P. Jagilinki, Irina Paluy, Vikas Nanda and Dror Noy日期:09/20/2021,浏览量:479,Q&A: 0

    Iron-sulfur proteins are ubiquitous among all living organisms and are indispensable for almost all metabolic pathways ranging from photosynthesis, respiration, nitrogen, and carbon dioxide cycles. The iron-sulfur clusters primarily serve as

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    Methods for the Extraction of Heme Prosthetic Groups from Hemoproteins
    从血红素中提取血红素辅基的方法
    作者:Kat Ellis-Guardiola, Jess Soule and Robert T. Clubb日期:09/20/2021,浏览量:679,Q&A: 0

    Hemoproteins are widely researched because they contain redox-active heme prosthetic groups (iron + protoporphyrin IX) that enable them to perform a range of vital functions, acting as enzymes, participants in electron transfer reactions, or gas

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    Cell-free Translation: Preparation and Validation of Translation-competent Extracts from Saccharomyces cerevisiae
    无细胞翻译:酿酒酵母翻译活性提取物的制备和验证
    作者:Brandon M. Trainor, Anton A. Komar, Dimitri G. Pestov and Natalia Shcherbik日期:09/20/2021,浏览量:593,Q&A: 0

    Cell-free translation is a powerful technique for in vitro protein synthesis. While cell-free translation platforms prepared from bacterial, plant, and mammalian cells are commercially available, yeast-based translation systems remain proprietary

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    Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays
    利用组蛋白肽阵列评估组蛋白修饰阅读蛋白的体外结合特异性
    作者:Mark W. Soo and Arneet L. Saltzman日期:09/20/2021,浏览量:198,Q&A: 0

    In the field of chromatin biology, a major goal of understanding the roles of histone post-translational modifications is to identify the proteins and domains that recognize these modifications. Synthetic histone peptides containing one or more

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    A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin
    一种高效生产和Src激酶介导的马海兔磷酸化的共纯化方法
    作者:Sherlene L. Brown, Yuan Ren, Daniel M. Suter and Seema Mattoo日期:09/20/2021,浏览量:286,Q&A: 0

    Cortactin is an actin-binding protein that regulates processes like cell migration, endocytosis, and tumor cell metastasis. Although cortactin is associated with actin-cytoskeletal dynamics in non-neuronal cells and cell-free systems, the exact

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    Comprehensive Identification of Translatable Circular RNAs Using Polysome Profiling
    利用多聚体谱综合鉴定可翻译环状RNA
    作者:Yanwen Ye, Zefeng Wang and Yun Yang日期:09/20/2021,浏览量:585,Q&A: 0
    [Abstract]

    Circular RNAs (circRNAs), a special type of RNAs without 5’- and 3’-ends, are widely present in eukaryotes and known to function as noncoding RNAs to regulate gene expression, including as miRNA sponges. Recent studies showed that many exonic circRNAs, generated by back-splicing of pre-mRNAs, can be translated in a cap-independent

    ...
    Anaerobic Expression and Purification of Holo-CCIS, an Artificial Iron-sulfur Protein
    人工铁硫蛋白 Holo-CCIS的厌氧表达与纯化
    作者:Bhanu P. Jagilinki, Irina Paluy, Vikas Nanda and Dror Noy日期:09/20/2021,浏览量:479,Q&A: 0
    [Abstract]

    Iron-sulfur proteins are ubiquitous among all living organisms and are indispensable for almost all metabolic pathways ranging from photosynthesis, respiration, nitrogen, and carbon dioxide cycles. The iron-sulfur clusters primarily serve as electron acceptors and donors and transfer electrons to active sites of various enzymes, thus driving the

    ...
    Methods for the Extraction of Heme Prosthetic Groups from Hemoproteins
    从血红素中提取血红素辅基的方法
    作者:Kat Ellis-Guardiola, Jess Soule and Robert T. Clubb日期:09/20/2021,浏览量:679,Q&A: 0
    [Abstract]

    Hemoproteins are widely researched because they contain redox-active heme prosthetic groups (iron + protoporphyrin IX) that enable them to perform a range of vital functions, acting as enzymes, participants in electron transfer reactions, or gas sensing, carrying, and storage proteins. While the heme prosthetic group is almost always essential for

    ...
    Cell-free Translation: Preparation and Validation of Translation-competent Extracts from Saccharomyces cerevisiae
    无细胞翻译:酿酒酵母翻译活性提取物的制备和验证
    作者:Brandon M. Trainor, Anton A. Komar, Dimitri G. Pestov and Natalia Shcherbik日期:09/20/2021,浏览量:593,Q&A: 0
    [Abstract]

    Cell-free translation is a powerful technique for in vitro protein synthesis. While cell-free translation platforms prepared from bacterial, plant, and mammalian cells are commercially available, yeast-based translation systems remain proprietary knowledge of individual labs. Here, we provide a detailed protocol for simple, fast, and

    ...
    Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays
    利用组蛋白肽阵列评估组蛋白修饰阅读蛋白的体外结合特异性
    作者:Mark W. Soo and Arneet L. Saltzman日期:09/20/2021,浏览量:198,Q&A: 0
    [Abstract]

    In the field of chromatin biology, a major goal of understanding the roles of histone post-translational modifications is to identify the proteins and domains that recognize these modifications. Synthetic histone peptides containing one or more modifications are a key tool to probe these interactions in pull-down assays with recombinant proteins

    ...
    A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin
    一种高效生产和Src激酶介导的马海兔磷酸化的共纯化方法
    作者:Sherlene L. Brown, Yuan Ren, Daniel M. Suter and Seema Mattoo日期:09/20/2021,浏览量:286,Q&A: 0
    [Abstract]

    Cortactin is an actin-binding protein that regulates processes like cell migration, endocytosis, and tumor cell metastasis. Although cortactin is associated with actin-cytoskeletal dynamics in non-neuronal cells and cell-free systems, the exact mechanisms underlying its fundamental roles in neuronal growth cones are not fully explored. Recent

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    Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids
    从针叶树类囊体中分离光合巨复合体和超复合体的增溶方法
    作者:Pushan Bag, Wolfgang P. Schröder, Stefan Jansson and Domenica Farci日期:09/05/2021,浏览量:513,Q&A: 0
    [Abstract]

    Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades. In higher plants, the process takes place in the thylakoid membranes where the two photosystems (PSI and PSII) are located. In the past few decades, the evolution of

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    Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (KD) and the Effective Concentration (EC50) of an Interplaying Effector Molecule Using Bio-layer Interferometry
    蛋白质-蛋白质复合物的动力学分析,以确定其电离常数 (KD) 和使用生物层干涉法相互作用的效应分子的有效浓度 (EC50)
    作者:Tim Orthwein, Luciano F. Huergo, Karl Forchhammer and Khaled A. Selim日期:09/05/2021,浏览量:1019,Q&A: 0
    [Abstract]

    Biolayer interferometry (BLI) is an emerging analytical tool that allows the study of protein complexes in real time to determine protein complex kinetic parameters. This article describes a protocol to determine the KD of a protein complex using a 6×His tagged fusion protein as bait immobilized on the NTA sensor chip of the

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    Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike
    SARS-CoV-2刺突慢病毒伪型载体制备和滴定的优化方法
    [Abstract]

    The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast and reliable protocol for the transient production of lentiviruses pseudotyped with SARS-CoV-2 Spike

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    Acid Hydrolysis for the Extraction of Archaeal Core Lipids and HPLC-MS Analysis
    酸水解法提取古生菌核心脂质及HPLC-MS分析
    作者:Maxime Tourte, Philippe Schaeffer, Vincent Grossi and Philippe M. Oger日期:08/20/2021,浏览量:397,Q&A: 0
    [Abstract]

    Lipid membranes are essential cellular elements as they provide cellular integrity and selective permeability under a broad range of environmental settings upon cell growth. In particular, Archaea are commonly recognized for their tolerance to extreme conditions, which is now widely accepted to stem from the unique structure of their lipids. While

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