神经科学

分类

    现刊
    In vivo Optical Access to Olfactory Sensory Neurons in the Mouse Olfactory Epithelium
    小鼠嗅上皮嗅觉感觉神经元的体内光学通路研究
    作者:Shigenori Inagaki, Ryo Iwata and Takeshi Imai日期:06/20/2021,浏览量:0,Q&A: 0

    In neuroscience, it is fundamental to understand how sensory stimuli are translated into neural activity at the entry point of sensory systems. In the olfactory system, odorants inhaled into the nasal cavity are detected by ~1,000 types of odorant

    ...
    Intravenous and Non-invasive Drug Delivery to the Mouse Basal Forebrain Using MRI-guided Focused Ultrasound
    MRI引导下聚焦超声对小鼠基底前脑静脉及无创给药影响的研究
    作者:Kristiana Xhima, Dallan McMahon, Edward Ntiri, Maged Goubran, Kullervo Hynynen and Isabelle Aubert日期:06/20/2021,浏览量:169,Q&A: 0

    Basal forebrain cholinergic neurons (BFCNs) regulate circuit dynamics underlying cognitive processing, including attention, memory, and cognitive flexibility. In Alzheimer’s disease and related neurodegenerative conditions, the degeneration of

    ...
    Enrichment of Vascular Fragments from Mouse Embryonic Brains for Endothelial Cell Analysis
    用于内皮细胞分析小鼠胚胎脑血管片段富集
    作者:Nicolas Santander and Thomas D. Arnold日期:06/20/2021,浏览量:0,Q&A: 0

    Endothelial cells in the brain interact with other cell types, forming the blood-brain barrier. This barrier controls the movement of solutes into and out of the brain, regulating pathophysiological processes and drug delivery to the brain. Common

    ...
    Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease
    用于研究人类疾病中蛋白质N-糖基化失调的N-糖肽丰度和N-糖基化位点的差异分析
    作者:Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin日期:06/20/2021,浏览量:0,Q&A: 0

    Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass

    ...
    Electrophysiological Properties of Neurons: Current-Clamp Recordings in Mouse Brain Slices and Firing-Pattern Analysis
    神经元的电生理特性:小鼠脑切片中的电流钳记录和放电模式分析
    作者:Elina Nagaeva, Ivan Zubarev and Esa R. Korpi日期:06/20/2021,浏览量:0,Q&A: 0

    Characterization of an electrically active cell, such as a neuron, demands measurement of its electrical properties. Due to differences in gene activation, location, innervation patterns, and functions, the millions of neurons in the mammalian brain

    ...
    A Lipidomics Approach to Measure Phosphatidic Acid Species in Subcellular Membrane Fractions Obtained from Cultured Cells
    用脂质组学方法测定培养细胞亚细胞膜组分中磷脂酸的种类
    作者:Nawal Kassas, Laetitia Fouillen, Stéphane Gasman and Nicolas Vitale日期:06/20/2021,浏览量:0,Q&A: 0

    Over the last decade, lipids have emerged as possessing an ever-increasing number of key functions, especially in membrane trafficking. For instance, phosphatidic acid (PA) has been proposed to play a critical role in different steps along the

    ...
    Dual Color, Live Imaging of Vesicular Transport in Axons of Cultured Sensory Neurons
    培养感觉神经元轴突囊泡运输的双色实时成像
    作者:Yoko Bekku and James L. Salzer日期:06/20/2021,浏览量:89,Q&A: 0

    The function of neurons in afferent reception, integration, and generation of electrical activity relies on their strikingly polarized organization, characterized by distinct membrane domains. These domains have different compositions resulting from

    ...
    In vivo Fluorescence Imaging of Extracellular ATP in the Mouse Cerebral Cortex with a Hybrid-type Optical Sensor
    混合型光传感器对小鼠大脑皮层细胞外ATP的体内荧光成像

    Adenosine 5’-triphosphate (ATP) works as an extracellular signaling molecule for cells in the brain, such as neurons and glia. Cellular communication via release of ATP is involved in a range of processes required for normal brain functions,

    ...
    Objective Quantitation of Focal Sweating Areas Using a Mouse Sweat-assay Model
    小鼠汗液分析模型对局部出汗区域的客观定量
    作者:Choongjin Ban and Dae-Hyuk Kwoen日期:06/05/2021,浏览量:169,Q&A: 0

    In vivo sweat quantitation assays are required for the development of drugs for the management of focal hyperhidrosis before clinical trials; however, in vivo assays, particularly mouse models, are rare. Even in sweat assays using mice, sweating is

    ...
    Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions
    培养神经元细胞成细胞体和神经突部分的机械分离
    作者:Ankita Arora, Raeann Goering, Hei-Yong G. Lo and J. Matthew Taliaferro日期:06/05/2021,浏览量:606,Q&A: 0

    Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules

    ...
    Generation and Maintenance of Homogeneous Human Midbrain Organoids
    同种人类中脑类器官的生成与维持
    作者:Henrik Renner, Martha Grabos, Hans R. Schöler and Jan M. Bruder日期:06/05/2021,浏览量:415,Q&A: 0

    Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a

    ...
    Dual Color, Live Imaging of Vesicular Transport in Axons of Cultured Sensory Neurons
    培养感觉神经元轴突囊泡运输的双色实时成像
    作者:Yoko Bekku and James L. Salzer日期:06/20/2021,浏览量:89,Q&A: 0
    [Abstract]

    The function of neurons in afferent reception, integration, and generation of electrical activity relies on their strikingly polarized organization, characterized by distinct membrane domains. These domains have different compositions resulting from a combination of selective targeting and retention of membrane proteins. In neurons, most proteins

    ...
    A Lipidomics Approach to Measure Phosphatidic Acid Species in Subcellular Membrane Fractions Obtained from Cultured Cells
    用脂质组学方法测定培养细胞亚细胞膜组分中磷脂酸的种类
    作者:Nawal Kassas, Laetitia Fouillen, Stéphane Gasman and Nicolas Vitale日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Over the last decade, lipids have emerged as possessing an ever-increasing number of key functions, especially in membrane trafficking. For instance, phosphatidic acid (PA) has been proposed to play a critical role in different steps along the secretory pathway or during phagocytosis. To further investigate in detail the precise nature of PA

    ...
    Electrophysiological Properties of Neurons: Current-Clamp Recordings in Mouse Brain Slices and Firing-Pattern Analysis
    神经元的电生理特性:小鼠脑切片中的电流钳记录和放电模式分析
    作者:Elina Nagaeva, Ivan Zubarev and Esa R. Korpi日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Characterization of an electrically active cell, such as a neuron, demands measurement of its electrical properties. Due to differences in gene activation, location, innervation patterns, and functions, the millions of neurons in the mammalian brain are tremendously diverse in their membrane characteristics and abilities to generate action

    ...
    Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease
    用于研究人类疾病中蛋白质N-糖基化失调的N-糖肽丰度和N-糖基化位点的差异分析
    作者:Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass spectrometry-based glycoproteomics technologies enabling large-scale N-glycoproteome profiling of

    ...
    Enrichment of Vascular Fragments from Mouse Embryonic Brains for Endothelial Cell Analysis
    用于内皮细胞分析小鼠胚胎脑血管片段富集
    作者:Nicolas Santander and Thomas D. Arnold日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Endothelial cells in the brain interact with other cell types, forming the blood-brain barrier. This barrier controls the movement of solutes into and out of the brain, regulating pathophysiological processes and drug delivery to the brain. Common isolation methods used to study these cells during embryonic development involve enzymatic treatment

    ...
    Intravenous and Non-invasive Drug Delivery to the Mouse Basal Forebrain Using MRI-guided Focused Ultrasound
    MRI引导下聚焦超声对小鼠基底前脑静脉及无创给药影响的研究
    作者:Kristiana Xhima, Dallan McMahon, Edward Ntiri, Maged Goubran, Kullervo Hynynen and Isabelle Aubert日期:06/20/2021,浏览量:169,Q&A: 0
    [Abstract]

    Basal forebrain cholinergic neurons (BFCNs) regulate circuit dynamics underlying cognitive processing, including attention, memory, and cognitive flexibility. In Alzheimer’s disease and related neurodegenerative conditions, the degeneration of BFCNs has long been considered a key player in cognitive decline. The cholinergic system thus

    ...
    In vivo Optical Access to Olfactory Sensory Neurons in the Mouse Olfactory Epithelium
    小鼠嗅上皮嗅觉感觉神经元的体内光学通路研究
    作者:Shigenori Inagaki, Ryo Iwata and Takeshi Imai日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    In neuroscience, it is fundamental to understand how sensory stimuli are translated into neural activity at the entry point of sensory systems. In the olfactory system, odorants inhaled into the nasal cavity are detected by ~1,000 types of odorant receptors (ORs) that are expressed by olfactory sensory neurons (OSNs). Since each OSN expresses only

    ...
    Generation and Maintenance of Homogeneous Human Midbrain Organoids
    同种人类中脑类器官的生成与维持
    作者:Henrik Renner, Martha Grabos, Hans R. Schöler and Jan M. Bruder日期:06/05/2021,浏览量:415,Q&A: 0
    [Abstract]

    Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a number of protocols for the generation of neural organoids have been published, most protocols require

    ...
    Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions
    培养神经元细胞成细胞体和神经突部分的机械分离
    作者:Ankita Arora, Raeann Goering, Hei-Yong G. Lo and J. Matthew Taliaferro日期:06/05/2021,浏览量:606,Q&A: 0
    [Abstract]

    Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules that encode them. A key question in the study of this process of RNA localization is the

    ...
    Objective Quantitation of Focal Sweating Areas Using a Mouse Sweat-assay Model
    小鼠汗液分析模型对局部出汗区域的客观定量
    作者:Choongjin Ban and Dae-Hyuk Kwoen日期:06/05/2021,浏览量:169,Q&A: 0
    [Abstract]

    In vivo sweat quantitation assays are required for the development of drugs for the management of focal hyperhidrosis before clinical trials; however, in vivo assays, particularly mouse models, are rare. Even in sweat assays using mice, sweating is quantitated by manually counting the number of sweating spots, which can contribute to various

    ...