分子生物学

分类

    现刊
    Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
    基于抗体系标记的简化表观基因组图谱
    作者:Steven Henikoff, Jorja G. Henikoff and Kami Ahmad日期:06/05/2021,浏览量:355,Q&A: 0
    [Abstract]

    We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples and single cells. With CUT&Tag, intact cells or nuclei are permeabilized, followed by successive

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    ATAC-Seq-based Identification of Extrachromosomal Circular DNA in Mammalian Cells and Its Validation Using Inverse PCR and FISH
    基于ATAC-Seq的哺乳动物细胞染色体外环状DNA鉴定及其反向PCR和FISH验证
    作者:Zhangli Su, Shekhar Saha, Teressa Paulsen, Pankaj Kumar and Anindya Dutta日期:05/05/2021,浏览量:755,Q&A: 0
    [Abstract]

    Recent studies from multiple labs including ours have demonstrated the importance of extrachromosomal circular DNA (eccDNA) from yeast to humans (Shibata et al., 2012; Dillon et al., 2015; Møller et al., 2016; Kumar et al., 2017; Turner et al., 2017; Kim et al., 2020). More recently, it has been found that cancer cells obtain a selective

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    A Sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA while Preserving Cellular DNA
    一种在保留细胞DNA的同时定量检测乙型肝炎病毒共价闭合环状(ccc)DNA的灵敏、特异PCR方法
    作者:Benno Zehnder, Stephan Urban and Thomas Tu日期:04/20/2021,浏览量:867,Q&A: 0
    [Abstract]

    Hepatitis B virus (HBV) is the major cause of liver diseases and liver cancer worldwide. After infecting hepatocytes, the virus establishes a stable episome (covalently closed circular DNA, or cccDNA) that serves as the template for all viral transcripts. Specific and accurate quantification of cccDNA is difficult because infected cells contain

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    A Novel Method to Construct Binary CRISPR Vectors for Plant Transformation by Single Round of PCR Amplification
    一种通过单轮PCR扩增构建用于植物转化的二元CRISPR载体的新方法
    作者:Kang Li, Yuhui Wang and Chuanying Fang日期:04/05/2021,浏览量:821,Q&A: 0
    [Abstract]

    CRISPR/Cas9 is an established and flexible tool for genome editing. However, most methods used to generate expression clones for the CRISPR/Cas9 are time-consuming. Hence, we have developed a one-step protocol to introduce sgRNA expression cassette(s) directly into binary vectors (Liu et al., 2020). In this approach, we have optimized the

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    Reconstitution of Chromatin by Stepwise Salt Dialysis
    逐步盐透析重组染色质
    作者:Grisel Cruz-Becerra and James T. Kadonaga日期:04/05/2021,浏览量:431,Q&A: 0
    [Abstract]

    Chromatin, rather than plain DNA, is the natural substrate of the molecular machines that mediate DNA-directed processes in the nucleus. Chromatin can be reconstituted in vitro by using different methodologies. The salt dialysis method yields chromatin that consists of purified histones and DNA. This biochemically pure chromatin is well-suited for

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    Real-time Base Excision Repair Assay to Measure the Activity of the 8-oxoguanine DNA Glycosylase 1 in Isolated Mitochondria of Human Skin Fibroblasts
    实时碱基切除修复法测定人皮肤纤维细胞的分离线粒体中8-氧鸟嘌呤DNA糖基化酶1的活性
    作者:Daniel Schniertshauer, Daniel Gebhard and Jörg Bergemann日期:03/20/2021,浏览量:754,Q&A: 0
    [Abstract]

    7,8-dihydro-8-oxoguanine (8-oxoG) is one of the most common and mutagenic oxidative DNA damages induced by reactive oxygen species (ROS). Since ROS is mainly produced in the inner membranes of the mitochondria, these organelles and especially the mitochondrial DNA (mtDNA) contained therein are particularly affected by this damage. Insufficient

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    Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy
    用原子力显微镜描述DNA解旋酶PriA与停止的DNA复制叉的相互作用
    作者:Yaqing Wang, Zhiqiang Sun, Piero R. Bianco and Yuri L. Lyubchenko日期:03/05/2021,浏览量:1348,Q&A: 0
    [Abstract]

    In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. ssDNA-binding protein (SSB) is typically present at the abandoned forks,

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    Defined Mutant Library Sequencing (DML-Seq) for Identification of Conditional Essential Genes
    确定突变体文库测序(DML-Seq)用于鉴定的条件必需基因
    作者:Shuai Shao, Lifan Wei, Feng Xia, Yuanxing Zhang and Qiyao Wang日期:03/05/2021,浏览量:701,Q&A: 0
    [Abstract]

    Transposon insertion sequencing (TIS) is an emerging technique which utilizes a massive transposon mutant library to screen specific phenotype and determine the conditional essential genetic requirements for bacterial fitness under distinct conditions combined with high-throughput parallel sequencing technology. Compared with a massive mutant

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    Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids
    受荧光标记和易于固化质粒影响的假单胞菌快速基因组工程
    作者:Daniel C. Volke, Nicolas T. Wirth and Pablo I. Nikel日期:02/20/2021,浏览量:2213,Q&A: 0
    [Abstract]

    Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted methods, homologous recombination, or λ

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    Histochemical Staining of Suberin in Plant Roots
    植物根部软木脂的组织化学染色
    作者:Peter Marhavý and Shahid Siddique日期:02/05/2021,浏览量:1179,Q&A: 0
    [Abstract]

    Histological stains are useful tools for characterizing cell shape, arrangement and the material they are made from. Stains can be used individually or simultaneously to mark different cell structures or polymers within the same cells, and to visualize them in different colors. Histological stains can be combined with genetically-encoded

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