分子生物学

分类

    现刊
    Construction of DNA/RNA Triplex Helices Based on GAA/TTC Trinucleotide Repeats
    基于GAA/TTC三核苷酸重复序列构建DNA/RNA三链螺旋
    作者:Jiahui Zhang, Ashkan Fakharzadeh, Feng Pan, Christopher Roland and Celeste Sagui日期:09/20/2021,浏览量:356,Q&A: 0
    [Abstract]

    Atypical DNA and RNA secondary structures play a crucial role in simple sequence repeat (SSR) diseases, which are associated with a class of neurological and neuromuscular disorders known as “anticipation diseases,” where the age of disease onset decreases and the severity of the disease is increased as the intergenerational expansion

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    En masse DNA Electroporation for in vivo Transcriptional Assay in Ascidian Embryos
    海鞘胚胎中DNA电穿孔的体内转录分析
    作者:Sébastien Darras日期:09/20/2021,浏览量:530,Q&A: 0
    [Abstract]

    Ascidian embryos are powerful models for functional genomics, in particular, due to the ease of generating a large number of transgenic embryos by electroporation. In addition, the small size of their genome makes them an attractive model for studying cis-regulatory elements that control gene expression during embryonic development. Here, I

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    Agrobacterium-mediated Transformation of Japonica Rice Using Mature Embryos and Regenerated Transgenic Plants
    农杆菌介导粳稻成熟胚和再生转基因植株的转化
    作者:Ammar Elakhdar, Masako Fukuda and Takahiko Kubo日期:09/20/2021,浏览量:342,Q&A: 0
    [Abstract]

    Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice. However, the efficiency of gene transfer by Agrobacterium in rice is low, particularly among japonica

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    In vitro Cleavage and Electrophoretic Mobility Shift Assays for Very Fast CRISPR
    快速CRISPR的体外裂解和电泳迁移率位移测定
    作者:Roger S. Zou, Yang Liu and Taekjip Ha日期:09/05/2021,浏览量:735,Q&A: 0
    [Abstract]

    CRISPR-Cas9 has transformed biomedical research and medicine through convenient and targeted manipulation of DNA. Time- and spatially-resolved control over Cas9 activity through the recently developed very fast CRISPR (vfCRISPR) system have facilitated comprehensive studies of DNA damage and repair. Understanding the fundamental principles of Cas9

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    Preparation and Characterization of Internally Modified DNA Templates for Chemical Transcription Roadblocking
    用于化学转录障碍的内部修饰 DNA 模板的制备和特征分析
    作者:Eric J. Strobel日期:09/05/2021,浏览量:405,Q&A: 0
    [Abstract]

    Site-specific transcription arrest is the basis of emerging technologies that assess nascent RNA structure and function. Cotranscriptionally folded RNA can be displayed from an arrested RNA polymerase (RNAP) for biochemical manipulations by halting transcription elongation at a defined DNA template position. Most transcription

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    Neutral Comet Assay to Detect and Quantitate DNA Double-Strand Breaks in Hematopoietic Stem Cells
    检测和定量造血干细胞DNA双链断裂的中性彗星试验
    作者:Irene Mariam Roy, Pon Sowbhagya Nadar and Satish Khurana日期:08/20/2021,浏览量:547,Q&A: 0
    [Abstract]

    In vertebrates, hematopoietic stem cells (HSCs) regulate the supply of blood cells throughout the lifetime and help to maintain homeostasis. Due to their long lifespan, genetic integrity is paramount for these cells, and accordingly, a number of stem cell-specific mechanisms are employed. However, HSCs tend to show more DNA damage with increasing

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    Implementing Novel Designs in pET Expression Plasmids that Increase Protein Production
    在pET表达质粒中实现增加蛋白质产量的新设计
    作者:Patrick J. Shilling and Daniel O. Daley日期:08/20/2021,浏览量:1376,Q&A: 0
    [Abstract]

    pET expression plasmids are widely used in the biotechnology, biopharmaceutical, and basic research sectors for the production of recombinant proteins. Typically, they are used off-the-shelf because they support high production titers; however, we have identified two design flaws in many pET plasmids that limit their production capacity. We used

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    CRISPR-mediated Labeling of Cells in Chick Embryos Based on Selectively Expressed Genes
    基于选择性表达基因的CRISPR介导的鸡胚胎细胞标记
    作者:Masahito Yamagata and Joshua R. Sanes日期:08/05/2021,浏览量:1266,Q&A: 0
    [Abstract]

    The abilities to mark and manipulate specific cell types are essential for an increasing number of functional, structural, molecular, and developmental analyses in model organisms. In a few species, this can be accomplished by germline transgenesis; in other species, other methods are needed to selectively label somatic cells based on the genes

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    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    利用本生烟叶片基因瞬时表达或沉默研究青枯菌毒力的快速简便方法
    作者:Wenjia Yu and Alberto P. Macho日期:08/05/2021,浏览量:822,Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed by observation and scoring of disease symptoms. Here, we describe a novel inoculation protocol to

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    A Gel-Based Assay for Probing Protein Translocation on dsDNA
    一种基于凝胶的检测dsDNA蛋白易位的方法
    作者:Christiane Brugger and Alexandra M. Deaconescu日期:07/20/2021,浏览量:1311,Q&A: 0
    [Abstract]

    Protein translocation on DNA represents the key biochemical activity of ssDNA translocases (aka helicases) and dsDNA translocases such as chromatin remodelers. Translocation depends on DNA binding but is a distinct process as it typically involves multiple DNA binding states, which are usually dependent on nucleotide binding/hydrolysis and are

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