免疫学

分类

    现刊
    Microscopic Detection of ASC Inflammasomes in Bone Marrow Derived Macrophages Post Stimulation
    刺激后骨髓源性巨噬细胞中ASC炎症小体的显微检测
    作者:Longjun Li, Rudi Mao and Xiaopeng Qi日期:09/05/2021,浏览量:840,Q&A: 0
    [Abstract]

    An inflammasome is an intracellular multiprotein complex that plays important roles in host defense and inflammatory responses. Inflammasomes are typically composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC), cytoplasmic sensor protein, and the effector protein pro-caspase-1. ASC assembly into a protein

    ...
    A Lipidomics Approach to Measure Phosphatidic Acid Species in Subcellular Membrane Fractions Obtained from Cultured Cells
    用脂质组学方法测定培养细胞亚细胞膜组分中磷脂酸的种类
    作者:Nawal Kassas, Laetitia Fouillen, Stéphane Gasman and Nicolas Vitale日期:06/20/2021,浏览量:1226,Q&A: 0
    [Abstract]

    Over the last decade, lipids have emerged as possessing an ever-increasing number of key functions, especially in membrane trafficking. For instance, phosphatidic acid (PA) has been proposed to play a critical role in different steps along the secretory pathway or during phagocytosis. To further investigate in detail the precise nature of PA

    ...
    In vitro and In vivo CD8+ T Cell Suppression Assays
    CD8+ T细胞体外和体内抑制试验
    作者:Lu Xie, Guihuan Liu, Yanjun Liu and Yuming Yu日期:05/20/2021,浏览量:2496,Q&A: 0
    [Abstract]

    CD8+CD28− T suppressor cells (Ts) have been documented to promote immune tolerance by suppressing effector T cell responses to alloantigens following transplantation. The suppressive function of T cells has been defined as the inhibitory effect of Ts on the proliferation rate of effector T cells. 3H-thymidine is a classical immunological

    ...
    A Simple and Robust Protocol for in vitro Differentiation of Mouse Non-pathogenic T Helper 17 Cells from CD4+ T Cells
    一种简单稳定的用小鼠CD4+ T细胞体外分化非致病性T辅助17细胞的方法
    作者:Siwen Kang, Ruohan Wu and Ruoning Wang日期:05/20/2021,浏览量:2063,Q&A: 0
    [Abstract]

    Functional and mechanistic studies of CD4+ T cell lineages rely on robust methods of in vitro T cell polarization. Here, we report an optimized protocol for in vitro differentiation of a mouse non-pathogenic T helper 17 (TH17) cell lineage. Most of the previously established protocols require irradiated splenocytes as artificial antigen presenting

    ...
    Brain-localized and Intravenous Microinjections in the Larval Zebrafish to Assess Innate Immune Response
    对斑马鱼幼体进行脑局部和静脉显微注射以评估先天免疫反应
    作者:Alison M. Rojas and Celia E. Shiau日期:04/05/2021,浏览量:2265,Q&A: 0
    [Abstract]

    Creating a robust and controlled infection model is imperative for studying the innate immune response. Leveraging the particular strengths of the zebrafish model system, such as optical transparency, ex utero development, and large clutch size, allows for the development of methods that yield consistent and reproducible results. We created a

    ...
    Ex vivo Assessment of Mitochondrial Function in Human Peripheral Blood Mononuclear Cells Using XF Analyzer
    使用XF分析仪体外评估人外周血单个核细胞线粒体功能
    作者:Alica Schöller-Mann, Katja Matt, Barbara Hochecker and Jörg Bergemann日期:04/05/2021,浏览量:2066,Q&A: 0
    [Abstract]

    Cellular health and function, as we know today, depend on a large extent on mitochondrial function. The essential function of mitochondria is the energy production, more precisely ATP production, via oxidative phosphorylation. Mitochondrial energy production parameters therefore represent important biomarkers. Studies on human cells have mainly

    ...
    Liposomal Clodronate-mediated Macrophage Depletion in the Zebrafish Model
    在斑马鱼模型中脂质体氯膦酸盐介导的巨噬细胞耗竭
    作者:Linlin Yang, Alison M. Rojas and Celia E. Shiau日期:03/20/2021,浏览量:2465,Q&A: 0
    [Abstract]

    The ability to conduct in vivo macrophage-specific depletion remains an effective means to uncover functions of macrophages in a wide range of physiological contexts. Compared to the murine model, zebrafish offer superior imaging capabilities due to their optical transparency starting from a single-cell stage to throughout larval development.

    ...
    Murine Monocyte and Macrophage Culture
    小鼠单核细胞和巨噬细胞培养
    作者:Simone M. Haag and Aditya Murthy日期:03/20/2021,浏览量:4091,Q&A: 0
    [Abstract]

    Myeloid progenitors in the bone marrow generate monocytes, macrophages, granulocytes and most dendritic cells. Even though these innate immune cells are part of the same lineage, each cell type plays a specific and critical role in tissue development, host defense and the generation of adaptive immunity. Protocols have been developed in the past

    ...
    A Versatile Protocol to Quantify BCR-mediated Phosphorylation in Human and Murine B Cell Subpopulations
    量化人类和小鼠B细胞亚群中BCR介导的磷酸化的通用实验方法
    作者:Jasper Rip, Rudi W. Hendriks and Odilia B. J. Corneth日期:02/05/2021,浏览量:1008,Q&A: 0
    [Abstract]

    Signal transduction is the process by which molecular signals are transmitted from the cell surface to its interior, resulting in functional changes inside the cell. B cell receptor (BCR) signaling is of crucial importance for B cells, as it regulates their differentiation, selection, survival, cellular activation and proliferation. Upon BCR

    ...
    In vitro STING Activation with the cGAMP-STINGΔTM Signaling Complex
    cGAMP-STINGΔTM信号复合物体外激活STING
    作者:Yanpu He, Celestine Hong, Darrell J. Irvine, Jiahe Li and Paula T. Hammond日期:02/05/2021,浏览量:2464,Q&A: 0
    [Abstract] Activating the STING (stimulator of interferon genes) signaling pathway via administration of STING agonist cyclic GMP-AMP (cGAMP) has shown great promise in cancer immunotherapy. While state-of-the-art approaches have predominantly focused on the encapsulation of cGAMP into liposomes or polymersomes for cellular delivery, we discovered that the ...