干细胞

分类

    现刊
    In situ Hybridization of miRNAs in Human Embryonic Kidney and Human Pluripotent Stem Cell-derived Kidney Organoids
    在人胚肾和人多能干细胞衍生的肾类器官中微小RNA的原位杂交
    作者:Filipa M. Lopes, Susan J. Kimber and Ioannis Bantounas日期:09/05/2021,浏览量:421,Q&A: 0
    [Abstract]

    MicroRNAs are small RNAs that negatively regulate gene expression and play an important role in fine-tuning molecular pathways during development. There is increasing interest in studying their function in the kidney, but the majority of studies to date use kidney cell lines and assess the total amounts of miRNAs of interest either by qPCR or by

    ...
    CRISPR/Cas9-mediated Precise SNP Editing in Human iPSC Lines
    CRISPR/Cas9介导的人iPSC细胞系SNP精确编辑
    作者:Hanwen Zhang and Siwei Zhang日期:06/20/2021,浏览量:1534,Q&A: 0
    [Abstract]

    Human induced pluripotent stem cells (hiPSCs) have been extensively used in the fields of developmental biology and disease modeling. CRISPR/Cas9 gene editing in iPSC lines often has a low frequency, which hampers its application in precise allele editing of disease-associated single nucleotide polymorphisms (SNPs), especially those in the

    ...
    Generation and Maintenance of Homogeneous Human Midbrain Organoids
    同种人类中脑类器官的生成与维持
    作者:Henrik Renner, Martha Grabos, Hans R. Schöler and Jan M. Bruder日期:06/05/2021,浏览量:2112,Q&A: 0
    [Abstract]

    Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a number of protocols for the generation of neural organoids have been published, most protocols require

    ...
    Single or Repeated Ablation of Mouse Olfactory Epithelium by Methimazole
    甲巯咪唑单次或多次消融小鼠嗅上皮细胞
    作者:Sofia Håglin, Staffan Bohm and Anna Berghard日期:04/20/2021,浏览量:2219,Q&A: 0
    [Abstract]

    Odor-detecting olfactory sensory neurons residing in the nasal olfactory epithelium (OE) are the only neurons in direct contact with the external environment. As a result, these neurons are subjected to chemical, physical, and infectious insults, which may be the underlying reason why neurogenesis occurs in the OE of adult mammals. This feature

    ...
    Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders
    人来源iPSC获得的神经祖细胞(NPCs)的产生作为神经和线粒体疾病的药物发现模型
    作者:Annika Zink, Pawel Lisowski and Alessandro Prigione日期:03/05/2021,浏览量:2568,Q&A: 1
    [Abstract]

    The high attrition rate in drug development processes calls for additional human-based model systems. However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced pluripotent stem cells (iPSCs) has revolutionized the field of neuronal disease modeling and drug discovery.

    ...
    Rapid and Simplified Induction of Neural Stem/Progenitor Cells (NSCs/NPCs) and Neurons from Human Induced Pluripotent Stem Cells (hiPSCs)
    从人诱导的多能干细胞(hiPSCs)快速简便地诱导神经干/祖细胞(NSCs/NPCs)和神经元
    作者:Ryutaro Kajihara, Tadahiro Numakawa and Takumi Era日期:02/05/2021,浏览量:2145,Q&A: 0
    [Abstract]

    Human induced pluripotent stem cells (iPSCs) and their progeny displaying tissue-specific characteristics have paved the way for regenerative medicine and research in various fields such as the elucidation of the pathological mechanism of diseases and the discovery of drug candidates. iPSC-derived neurons are particularly valuable as it is

    ...
    Histological and Immunohistochemical Examination of Stem Cell Proliferation and Reepithelialization in the Wounded Skin
    损伤皮肤干细胞增殖和再生的组织学和免疫组化检测
    作者:Rupali Gund, Ravindra Zirmire, Haarshaadri J., Gaurav Kansagara and Colin Jamora日期:01/20/2021,浏览量:2617,Q&A: 0
    [Abstract]

    The skin is the largest organ that protects our body from the external environment and it is constantly exposed to pathogenic insults and injury. Repair of damage to this organ is carried out by a complex process involving three overlapping phases of inflammation, proliferation and remodeling. Histological analysis of wounded skin is a convenient

    ...
    Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Osteoclasts
    人诱导多能干细胞分化破骨细胞的研究
    作者:I-Ping Chen日期:12/20/2020,浏览量:2000,Q&A: 0
    [Abstract]

    Defects in bone resorption by osteoclasts result in numerous rare genetic bone disorders as well as in some common diseases such as osteoporosis or osteopetrosis. The use of hiPSC-differentiated osteoclasts opens new avenues in this research field by providing an unlimited cell source and overcoming obstacles such as unavailability of human

    ...
    Protocol for Isolation, Stimulation and Functional Profiling of Primary and iPSC-derived Human NK Cells
    原代和iPSC来源的人类NK细胞的分离、刺激和功能分析方案
    [Abstract]

    Natural killer (NK) cells are innate immune cells, characterized by their cytotoxic capacity, and chemokine and cytokine secretion upon activation. Human NK cells are identified by CD56 expression. Circulating NK cells can be further subdivided into the CD56bright (~10%) and CD56dim NK cell subsets (~90%). NK cell-like cells can also be derived

    ...
    Derivation of Induced Pluripotent Stem Cells from Human Fibroblasts Using a Non-integrative System in Feeder-free Conditions
    无饲养层条件下利用非整合系统从人成纤维细胞中衍生诱导多能干细胞
    作者:Alvaro A. Beltran, Sarahi G. Molina and Adriana S. Beltran日期:10/20/2020,浏览量:2288,Q&A: 0
    [Abstract] Induced pluripotent stem cells (iPSCs) are genetically reprogrammed somatic cells that exhibit features identical to those of embryonic stem cells (ESCs). Multiple approaches are available to derive iPSCs, among which the Sendai virus is the most effective at reprogramming different cell types. Here we describe a rapid, efficient, safe, and ...