免疫学

分类

    现刊
    Isolation of Microvesicles from Human Circulating Neutrophils
    人循环中性粒细胞微泡的分离
    作者:Dong Zhan, Ellie McConachie, Steven W. Edwards, Helen L. Wright, Robert J. Moots and Sittisak Honsawek日期:10/05/2021,浏览量:810,Q&A: 0
    [Abstract]

    Neutrophil-derived microvesicles (NDMVs) are liberated by neutrophils upon cell activation by molecules. Once activated, neutrophils are primarily involved in acute inflammation; however, the microvesicles they produce are largely anti-inflammatory. NDMVs have been shown to protect cartilage during inflammatory arthritis. They exert these effects

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    Isolation and Quantification of Mouse γδT-cells in vitro and in vivo
    体外和体内小鼠γδT细胞的分离和定量
    作者:Isha Rana, Krithika Badarinath, Ravindra K. Zirmire and Colin Jamora日期:09/05/2021,浏览量:992,Q&A: 0
    [Abstract]

    The skin plays an important role in protecting the body from pathogens and chemicals in the external environment. Upon injury, a healing program is rapidly initiated and involves extensive intercellular communication to restore tissue homeostasis. The deregulation of this crosstalk can lead to abnormal healing processes and is the foundation of

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    Preparation of Drosophila Larval Blood Cells for Single-cell RNA Sequencing
    果蝇幼虫血细胞单细胞RNA测序的制备
    作者:Sudhir Gopal Tattikota and Norbert Perrimon日期:08/20/2021,浏览量:650,Q&A: 0
    [Abstract]

    Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain high-quality single cells in suspension. Recently, we used this approach to characterize Drosophila blood

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    Isolation of Microglia and Analysis of Protein Expression by Flow Cytometry: Avoiding the Pitfall of Microglia Background Autofluorescence
    小胶质细胞的分离及蛋白表达的流式细胞仪分析:避免小胶质细胞背景荧光的陷阱
    作者:Jeremy C. Burns, Richard M. Ransohoff and Michaël Mingueneau日期:07/20/2021,浏览量:1642,Q&A: 0
    [Abstract]

    Microglia are a unique type of tissue-resident innate immune cell found within the brain, spinal cord, and retina. In the healthy nervous system, their main functions are to defend the tissue against infectious microbes, support neuronal networks through synapse remodeling, and clear extracellular debris and dying cells through phagocytosis. Many

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    Production, quantification, and infection of Amazonian Phlebovirus (Bunyaviridae)
    亚马孙流域白蛉病毒属(布尼亚病毒科)的产生、定量和感染
    作者:Carolina Torturella Rath, Aislan de Carvalho Vivarini, Renata M. Pereira and Ulisses Gazos Lopes日期:07/05/2021,浏览量:1426,Q&A: 0
    [Abstract]

    Phlebotomine vectors, sand flies of the order Diptera, are known to transmit Leishmania parasites as well as RNA viruses (arboviruses) to humans. The arbovirus, Icoaraci Phlebovirus (BeAN 24262 - ICOV), used in this study was isolated from Nectomys rodents, a mammalian species that is the same natural sylvatic reservoir of Leishmania (Leishmania)

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    Murine Monocyte and Macrophage Culture
    小鼠单核细胞和巨噬细胞培养
    作者:Simone M. Haag and Aditya Murthy日期:03/20/2021,浏览量:4115,Q&A: 0
    [Abstract]

    Myeloid progenitors in the bone marrow generate monocytes, macrophages, granulocytes and most dendritic cells. Even though these innate immune cells are part of the same lineage, each cell type plays a specific and critical role in tissue development, host defense and the generation of adaptive immunity. Protocols have been developed in the past

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    Metabolomic and Lipidomic Analysis of Bone Marrow Derived Macrophages
    骨髓衍生巨噬细胞的代谢组学和脂质组学分析
    作者:Gretchen L. Seim, Steven V. John and Jing Fan日期:07/20/2020,浏览量:3648,Q&A: 0
    [Abstract] Macrophages are highly plastic immune cells that are capable of adopting a wide array of functional phenotypes in response to environmental stimuli. The changes in macrophage function are often supported and regulated by changes in cellular metabolism. Capturing a comprehensive picture of metabolism is vital for understanding the role of metabolic ...
    Generation of T cells from Human and Nonhuman Primate Pluripotent Stem Cells
    从人类和非人类灵长类动物多能干细胞中产生T细胞
    作者:Akhilesh Kumar, Saritha S. D’Souza, Gene Uenishi, Mi Ae Park, Jeong Hee Lee and Igor I. Slukvin日期:07/05/2020,浏览量:3340,Q&A: 0
    [Abstract] Pluripotent stem cells (PSCs) have the potential to provide homogeneous cell populations of T cells that can be grown at a clinical scale and genetically engineered to meet specific clinical needs. OP9-DLL4, a stromal line ectopically expressing the Notch ligand Delta-like 4 (DLL4) is used to support differentiation of PSCs to T-lymphocytes. This ...
    Isolation and High Throughput Flow Cytometric Apoptosis Assay of Human Neutrophils to Enable Compound Library Screening
    人中性粒细胞的分离及高通量流式细胞术凋亡分析用于复合文库筛选
    作者:K. D. Herman, A. Rahman and L. R. Prince日期:06/05/2020,浏览量:2441,Q&A: 0
    [Abstract] The study of human neutrophils in vitro is challenging due to their short half-life and propensity for activation. However, with careful handling and manipulation in the laboratory, they can be a powerful tool to investigate immune responses in health and disease. Here we describe a method for the isolation of human neutrophils from ...
    Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice
    结核分枝杆菌感染小鼠肺和支气管肺泡灌洗样品中髓样细胞的流式细胞仪分析和荧光激活细胞分选
    作者:Alissa C. Rothchild, Dat Mai, Alan Aderem and Alan H. Diercks日期:05/20/2020,浏览量:3177,Q&A: 0
    [Abstract] Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing the early events of in vivo infection following aerosol challenge is critical for ...