细胞生物学

分类

    现刊
    Isolation and Characterization of Membrane Vesicles from Lactobacillus Species
    乳酸杆菌属膜囊泡中的分离和特性研究
    作者:Julie C. Caruana, Scott N. Dean and Scott A. Walper日期:09/05/2021,浏览量:764,Q&A: 0
    [Abstract]

    Throughout their life cycle, bacteria shed portions of their outermost membrane comprised of proteins, lipids, and a diversity of other biomolecules. These biological nanoparticles have been shown to have a range of highly diverse biological activities, including pathogenesis, community regulation, and cellular defense (among others). In recent

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    Protein Import Assay into Mitochondria Isolated from Human Cells
    从人细胞中分离的线粒体蛋白质输入测定
    作者:Lena M. Murschall, Esra Peker, Thomas MacVicar, Thomas Langer and Jan Riemer日期:06/20/2021,浏览量:1472,Q&A: 0
    [Abstract]

    Mitochondria are essential organelles containing approximately 1,500 proteins. Only approximately 1% of these proteins are synthesized inside mitochondria, whereas the remaining 99% are synthesized as precursors on cytosolic ribosomes and imported into the organelle. Various tools and techniques to analyze the import process have been developed.

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    Preparation of Synaptoneurosomes to Study the Synapse in the Murine Cerebral Cortex
    用于研究小鼠大脑皮层突触的突触神经小体的制备
    作者:Ariel Diaz, Enrique Torre and Manuel Yepes日期:01/20/2021,浏览量:1488,Q&A: 1
    [Abstract]

    The synapse is a complex structure where the transmission of information takes place. Synaptic dysfunction is one of the earliest pathophysiological events in several diseases, such as traumatic brain injury, cerebral ischemia, and neurodegenerative diseases. Thus, a methodology to study synaptic structure and function is crucial for the

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    Lipid Droplet Isolation from Arabidopsis thaliana Leaves
    拟南芥叶脂滴的分离
    作者:Yovanny Izquierdo, Rubén Fernández-Santos, Tomás Cascón and Carmen Castresana日期:12/20/2020,浏览量:1473,Q&A: 0
    [Abstract]

    Lipid droplets (LDs) are neutral lipid aggregates surrounded by a phospholipid monolayer and specific proteins. In plants, they play a key role as energy source after seed germination, but are also formed in vegetative tissues in response to developmental or environmental conditions, where their functions are poorly understood. To elucidate these,

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    Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels
    使用聚丙烯酰胺凝胶生成巨型磷脂囊泡(GUVs)
    作者:Eric Parigoris, Daniel L. Dunkelmann and Unai Silvan日期:11/05/2020,浏览量:1943,Q&A: 0
    [Abstract] Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics. While several protocols have been developed for their generation in recent years, the use of these techniques involving charged lipid types and buffers of physiological ionic ...
    Fluorescent Polysome Profiling in Caenorhabditis elegans
    秀丽隐杆线虫荧光多聚体分析
    作者:Dan Shaffer and Jarod A Rollins日期:09/05/2020,浏览量:2684,Q&A: 0
    [Abstract] An important but often overlooked aspect of gene regulation occurs at the level of protein translation. Many genes are regulated not only by transcription but by their propensity to be recruited to actively translating ribosomes (polysomes). Polysome profiling allows for the separation of unbound 40S and 60S subunits, 80S monosomes, and actively ...
    Stopped-flow Light Scattering Analysis of Red Blood Cell Glycerol Permeability
    红细胞甘油通透性的Stopped-flow 光散射分析
    作者:Patrizia Gena, Piero Portincasa, Sabino Matera, Yonathan Sonntag, Michael Rutzler and Giuseppe Calamita日期:08/20/2020,浏览量:1912,Q&A: 0
    [Abstract] Stopped-Flow Light Scattering (SFLS) is a method devised to analyze the kinetics of fast chemical reactions that result in a significant change of the average molecular weight and/or in the shape of the reaction substrates. Several modifications of the original stopped-flow system have been made leading to a significant extension of its technical ...
    Buoyant Density Fractionation of Small Extracellular Vesicle Sub-populations Derived from Mammalian Cells
    哺乳动物细胞来源的胞外小囊泡亚群浮力密度分级
    作者:Morayma M. Temoche-Diaz, Matthew J. Shurtleff and Randy Schekman日期:08/05/2020,浏览量:2175,Q&A: 0
    [Abstract] Small extracellular vesicles (sEVs) encompass a variety of distinct vesicles that are secreted to the extracellular space. Many methodologies currently used for EV isolation (e.g., differential ultracentrifugation concluding in a high-speed pellet, precipitation by macromolecular crowding agents or size excusion chromatography–SEC) do not ...
    Measuring Intracellular Vesicle Density and Dispersion Using Fluorescence Microscopy and ImageJ/FIJI
    使用荧光显微镜和ImageJ / FIJI测量细胞内囊泡的密度和分散度
    [Abstract] Cell signalling, cell secretion, and plasma membrane repair are processes that critically rely on intracellular vesicles, important components of the endocytic and secretory pathways. More specifically, the strategic distribution of intracellular vesicles is important for diverse cellular processes. The method presented here is a simple, ...
    Extracellular Vesicles Tracking and Quantification Using CT and Optical Imaging in Rats
    大鼠细胞外囊泡的CT和光学成像追踪与定量研究
    作者:Shaowei Guo, Oshra Betzer, Nisim Perets, Shira Landau, Daniel Offen, Rachela Popovtzer and Shulamit Levenberg日期:06/05/2020,浏览量:2371,Q&A: 0
    [Abstract] Exosomes, a subtype of extracellular vesicles, are nanovesicles of endocytic origin. Exosomes contain a plethora of proteins, lipids, and genetic materials of parent cells to facilitate intercellular communications. Tracking exosomes in vivo is fundamentally important to understand their biodistribution pattern and the mechanism of ...