植物科学

分类

    现刊
    Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids
    从针叶树类囊体中分离光合巨复合体和超复合体的增溶方法
    作者:Pushan Bag, Wolfgang P. Schröder, Stefan Jansson and Domenica Farci日期:09/05/2021,浏览量:435,Q&A: 0
    [Abstract]

    Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades. In higher plants, the process takes place in the thylakoid membranes where the two photosystems (PSI and PSII) are located. In the past few decades, the evolution of

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    Histological Methods to Detect Early-stage Plant Defense Responses during Artificial Inoculation of Lolium perenne with Epichloë festucae
    羊茅人工接种多年生黑麦草过程中植物早期防御反应的组织学检测
    作者:Mostafa Rahnama, Damien J. Fleetwood and Richard D. Johnson日期:05/05/2021,浏览量:3344,Q&A: 0
    [Abstract]

    Epichloë species form agriculturally important symbioses with many cool season grasses. To study these symbioses, such as the interaction of Epichloë festucae with perennial ryegrass (Lolium perenne), host plants can be infected by artificial inoculation of etiolated seedlings. This inoculation is performed by placing mycelium into an

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    Characterising Plant Deubiquitinases with in vitro Activity-based Labelling and Ubiquitin Chain Disassembly Assays
    植物去泛素酶的体外活性标记和泛素链分解分析
    作者:Michael J. Skelly and Steven H. Spoel日期:05/05/2021,浏览量:2217,Q&A: 0
    [Abstract]

    Post-translational modification of proteins by ubiquitin is an essential cellular signaling mechanism in all eukaryotes. Ubiquitin is removed from target proteins by a wide range of deubiquitinase (DUB) enzymes with different activities and substrate specificities. Understanding how DUBs function in vitro is a vital first step to uncovering their

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    In vitro Reconstitution Assays of Arabidopsis 20S Proteasome
    拟南芥20S蛋白酶体的体外重组分析
    作者:Yanjun Li, Di Sun, Xingxing Yan, Zhiye Wang and Xiuren Zhang日期:04/05/2021,浏览量:2932,Q&A: 0
    [Abstract]

    The majority of cellular proteins are degraded by the 26S proteasome in eukaryotes. However, intrinsically disordered proteins (IDPs), which contain large portions of unstructured regions and are inherently unstable, are degraded via the ubiquitin-independent 20S proteasome. Emerging evidence indicates that plant IDP homeostasis may also be

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    Atomic Force Microscopy to Characterize Ginger Lipid-Derived Nanoparticles (GLDNP)
    原子力显微镜研究生姜脂质纳米颗粒(GLDNP)
    作者:Dingpei Long, Chunhua Yang, Junsik Sung and Didier Merlin日期:04/05/2021,浏览量:2774,Q&A: 0
    [Abstract]

    We have demonstrated that a specific population of ginger-derived nanoparticles (GDNP-2) could effectively target the colon, reduce colitis, and alleviate colitis-associated colon cancer. Naturally occurring GDNP-2 contains complex bioactive components, including lipids, proteins, miRNAs, and ginger secondary metabolites (gingerols and shogaols).

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    Chromatographic Analysis for Targeted Metabolomics of Antioxidant and Flavor-Related Metabolites in Tomato
    番茄抗氧化剂和风味相关代谢产物靶向代谢组学的色谱分析
    作者:Nergiz Gürbüz Çolak, Neslihan Tek Eken, Anne Frary and Sami Doğanlar日期:03/05/2021,浏览量:2210,Q&A: 0
    [Abstract]

    Targeted metabolomics is a useful approach to evaluate crop breeding studies. Antioxidant and flavor-related traits are of increasing interest and are considered quality traits in tomato breeding. The present study presents chromatographic methods to study antioxidants (carotenoids, vitamin C, vitamin E, phenolic compounds, and glutathione) and

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    Using 14C-acetate Pulse-chase Labeling to Study Fatty Acid and Glycerolipid Metabolism in Plant Leaves
    用14C -乙酸酯脉冲追逐标记法研究植物叶片中脂肪酸和甘油脂代谢
    作者:Linhui Yu, Chao Zhou, Jilian Fan and Changcheng Xu日期:02/05/2021,浏览量:1649,Q&A: 0
    [Abstract]

    Lipids metabolism is comprised of networks of reactions occurred in different subcellular compartments. Isotopic labeling is a good way to track the transformations and movements of metabolites without perturbing overall cellular metabolism. Fatty acids, the building blocks of membrane lipids and storage triacylglycerols, are synthesized in

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    Non-radioactive Assay to Determine Product Profile of Short-chain Isoprenyl Diphosphate Synthases
    用于确定短链异戊烯基二磷酸合酶产物谱的非放射性分析
    作者:Avanish Rai and Dinesh A. Nagegowda日期:01/05/2021,浏览量:1199,Q&A: 0
    [Abstract] Isoprenoids represent the largest class of metabolites with amazing diversities in structure and function. They are involved in protecting plants against pathogens or herbivores or involved in attracting pollinators. Isoprenoids are derived from geranyl diphosphate (GPP; C10), farnesyl diphosphate (FPP; C15), geranylgeranyl ...
    Analysis of Isotopically-labeled Monogalactosyldiacylglycerol Molecular Species from [14C]Acetate-Labeled Tobacco Leaves
    [14C]醋酸标记烟叶中同位素标记的单半乳糖基二酰甘油分子组成分析
    作者:Hari Kiran Kotapati and Philip D. Bates日期:12/20/2020,浏览量:997,Q&A: 0
    [Abstract]

    Plant lipid metabolism is a dynamic network where synthesis of essential membrane lipids overlaps with synthesis of valuable storage lipids (e.g., vegetable oils). Monogalactosyldiacylglycerol (MGDG) is a key component of the chloroplast membrane system required for photosynthesis and is produced by multiple pathways within the lipid metabolic

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    Pipecolic Acid Quantification Using Gas Chromatography-coupled Mass Spectrometry
    哌啶酸的气相色谱-质谱定量分析
    作者:Keshun Yu, Huazhen Liu and Pradeep Kachroo日期:12/05/2020,浏览量:1514,Q&A: 0
    [Abstract]

    Pipecolic acid (Pip), a non-proteinacious product of lysine catabolism, is an important regulator of immunity in plants and humans alike. For instance, Pip accumulation is associated with the genetic disorder Zellweger syndrome, chronic liver diseases, and pyridoxine-dependent epilepsy in humans. In plants, Pip accumulates upon pathogen infection

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