植物科学

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    现刊
    Isolation of Plant Nuclei Compatible with Microfluidic Single-nucleus ATAC-sequencing
    与微流体单核 ATAC 测序兼容的植物核的分离
    作者:Sandra B. Thibivilliers, Dirk K. Anderson and Marc Y. Libault日期:12/05/2021,浏览量:643,Q&A: 0
    [Abstract]

    Gene expression depends on the binding of transcription factors with DNA regulatory sequences. The level of accessibility for these sequences varies between cells and cell types. Until recently, using the Tn5 assay for transposase-accessible chromatin for sequencing (ATAC-seq) technology allowed assessing the profiles of chromatin from an entire

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    Split-luciferase Complementation Imaging Assay to Study Protein-protein Interactions in Nicotiana benthamiana
    分裂荧光素酶互补成像分析研究本氏烟草中的蛋白质之间相互作用
    作者:Liping Wang, Gang Yu, Alberto P. Macho and Rosa Lozano-Durán日期:12/05/2021,浏览量:869,Q&A: 0
    [Abstract]

    The experimental identification of protein-protein interactions (PPIs) is critical to understand protein function. Thus, a plethora of sensitive and versatile approaches have been developed to detect PPIs in vitro or in vivo, such as protein pull-down, yeast two-hybrid (Y2H), co-immunoprecipitation (co-IP), and bimolecular fluorescence

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    Enrichment of Cytoplasmic RNA Granules from Arabidopsis Seedlings
    从拟南芥幼苗中富集细胞质 RNA 颗粒
    作者:Zhen Lei, Eun Yu Kim and Jungnam Cho日期:11/05/2021,浏览量:784,Q&A: 0
    [Abstract]

    RNA granules (RGs) are membraneless intracellular compartments that play important roles in the post-transcriptional control of gene expression. Stress granules (SGs) are a type of RGs that form under environmental challenges and/or internal cellular stresses. Stress treatments lead to strong mRNAs translational inhibition and storage in SGs until

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    Histone modification ChIP-seq on Arabidopsis thaliana Plantlets
    拟南芥植株上的组蛋白修饰ChIP-seq
    作者:Juan S. Ramirez-Prado, David Latrasse and Moussa Benhamed日期:11/05/2021,浏览量:918,Q&A: 1
    [Abstract]

    Characterizing the molecular mechanisms regulating gene expression is crucial for understanding the regulatory processes underlying physiological responses to environmental and developmental signals in eukaryotes. The covalent modification of histones contributes to the compaction levels of chromatin, as well as the recruitment of the

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    Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing
    利用DNA测序对植物线粒体或叶绿体特定位点RNA编辑的定量分析
    作者:Yang Yang and Weixing Shan日期:09/20/2021,浏览量:737,Q&A: 0
    [Abstract]

    Cytidine-to-uridine (C-to-U) RNA editing is one of the most important post-transcriptional RNA processing in plant mitochondria and chloroplasts. Several techniques have been developed to detect the RNA editing efficiency in plant mitochondria and chloroplasts, such as poisoned primer extension (PPE) assays, high-resolution melting (HRM) analysis,

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    U2.3 Precursor Small Nuclear RNA in vitro Processing Assay
    U2.3前体小核 RNA体外加工试验
    作者:Chan Lin, Yujie Feng, Xueyan Peng, Jiaming Wu, Weili Wang and Yunfeng Liu日期:09/05/2021,浏览量:646,Q&A: 0
    [Abstract]

    Small nuclear RNAs (snRNAs) are vital for eukaryotic cell activities and play important roles in pre-mRNA splicing. The molecular mechanism underlying the transcription of snRNA, regulated via upstream/downstream cis-elements and relevant trans-elements, has been investigated in detail using cell-free extracts. However, the processing of precursor

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    A Novel Method to Map Small RNAs with High Resolution
    一种新的高分辨率小RNA图谱绘制方法
    作者:Kun Huang, Feray Demirci, Blake C. Meyers and Jeffrey L. Caplan日期:08/20/2021,浏览量:1289,Q&A: 0
    [Abstract]

    Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular molecule dynamics in situ. However, the application of super-resolution imaging techniques to detect

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    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    利用本生烟叶片基因瞬时表达或沉默研究青枯菌毒力的快速简便方法
    作者:Wenjia Yu and Alberto P. Macho日期:08/05/2021,浏览量:1365,Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed by observation and scoring of disease symptoms. Here, we describe a novel inoculation protocol to

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    Measurement of Transgene Copy Number in Plants Using Droplet Digital PCR
    应用微滴数字PCR技术测定植物中的转基因拷贝数
    作者:Yao-Min Cai, Quentin M. Dudley and Nicola J. Patron日期:07/05/2021,浏览量:1843,Q&A: 0
    [Abstract]

    Transgenic plants are produced both to investigate gene function and to confer desirable traits into crops. Transgene copy number is known to influence expression levels, and consequently, phenotypes. Similarly, knowledge of transgene zygosity is desirable for making quantitative assessments of phenotype and tracking the inheritance of transgenes

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    Micrografting in Arabidopsis Using a Silicone Chip
    利用硅芯片进行拟南芥微嫁接
    作者:Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi日期:06/20/2021,浏览量:2393,Q&A: 1
    [Abstract]

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including defense mechanisms and responses to surrounding environments such as soil and light conditions.

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