植物科学

分类

    现刊
    Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120
    反义RNA介导的鱼腥藻PCC 7120基因敲除株的构建
    作者:Amit Srivastava, Anand Ballal, Karl Forchhammer and Anil Kumar Tripathi日期:02/20/2020,浏览量:85,Q&A: 0
    [Abstract] Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many genes in Anabaena encode an essential life function and hence cannot be deleted, causing a ...
    Measuring Protein Half-life in Arabidopsis thaliana
    拟南芥蛋白半衰期的检测
    作者:Hongqing Guo and Yanhai Yin日期:08/05/2019,浏览量:792,Q&A: 0
    [Abstract] Post-translational modifications play important roles in controlling protein function and can lead to altered protein stability. Protein stability can be determined after treatment with the protein synthesis inhibitor Cycloheximide. Cycloheximide is a translational inhibitor that inhibits protein synthesis via cytoplasmic ribosomes. Here we ...
    Extraction and Purification of Laccases from Rice Stems
    水稻茎秆酶的提取与纯化
    作者:Chenna Swetha and P. V. Shivaprasad日期:04/05/2019,浏览量:1745,Q&A: 0
    [Abstract] Laccases are found in cell walls of plants in very low amounts. This protocol provides an efficient method to purify laccases from rice stems. The method involves three steps: 1) Isolation of total protein from rice stems using buffers with high salt concentration to extract protein from cell walls; 2) Purification of laccases using concanavalin-A ...
    In vitro Protein-DNA Binding Assay (AlphaScreen® Technology)
    蛋白质-DNA结合的体外检测法 (AlphaScreen® Technology)
    作者:Mika Nomoto, Yasuomi Tada and Hironaka Tsukagoshi日期:02/05/2019,浏览量:2464,Q&A: 0
    [Abstract] Identification of specific DNA binding sites of transcription factors is important in understanding their functions. Recent techniques allow us to investigate genome-wide in vivo binding positions by chromatin immunoprecipitation combined with high-throughput sequencing. However, to further explore the binding motifs of transcription ...
    Genome-wide Estimation of Evolutionary Distance and Phylogenetic Analysis of Homologous Genes
    进化距离的全基因组评估和同源基因的系统进化分析
    作者:Meixia Zhao, Biao Zhang, Jianxin Ma and Damon Lisch日期:12/05/2018,浏览量:2638,Q&A: 0
    [Abstract] Homologous genes, including paralogs and orthologs, are genes that share sequence homologies within or between different species. Homologous genes originate from a common origin through speciation, genetic duplication or horizontal gene transfer. Estimation of the sequence divergence of homologous genes help us to understand divergence time, which ...
    Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling
    利用拟南芥叶肉细胞原生质体研究未折叠蛋白响应信号
    作者:Yan Bao and Diane C. Bassham日期:12/05/2018,浏览量:2387,Q&A: 0
    [Abstract] Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded. This is termed ER stress, and triggers the unfolded protein response (UPR). Assays for activation of the UPR in plants include Tunicamycin (Tm)- or dithiothreitol ...
    Real-time PCR Analysis of PAMP-induced Marker Gene Expression in Nicotiana benthamiana
    本塞姆氏烟草中PAMP诱导标记基因表达的实时定量PCR分析
    作者:Fan Liu, Yuanpeng Xu, Yan Wang and Yuanchao Wang日期:10/05/2018,浏览量:2938,Q&A: 0
    [Abstract] Perception of pathogen-associated molecular patterns (PAMPs) often triggers various innate immune responses in plants. The transcriptional changes of defense-related genes are often used as a marker to assay PAMP-triggered plant immune response. Here we described a protocol to monitor the relative expression level of marker genes in Nicotiana ...
    In planta Transcriptome Analysis of Pseudomonas syringae
    丁香假单胞菌的植物转录组分析
    作者:Tatsuya Nobori and Kenichi Tsuda日期:09/05/2018,浏览量:3860,Q&A: 0
    [Abstract] Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv. tomato DC3000, in the leaves of Arabidopsis thaliana at an early stage of ...
    Tethered Chromosome Conformation Capture Sequencing in Triticeae: A Valuable Tool for Genome Assembly
    小麦族系中染色体构象捕获测序:一个有价值的基因组组装工具
    作者:Axel Himmelbach, Ines Walde, Martin Mascher and Nils Stein日期:08/05/2018,浏览量:3576,Q&A: 0
    [Abstract] Chromosome conformation capture sequencing (Hi-C) is a powerful method to comprehensively interrogate the three-dimensional positioning of chromatin in the nucleus. The development of Hi-C can be traced back to successive increases in the resolution and throughput of chromosome conformation capture (3C) (Dekker et al., 2002). The basic ...
    High Resolution Melting Temperature Analysis to Identify CRISPR/Cas9 Mutants from Arabidopsis
    高分辨率熔解温度分析鉴定拟南芥中的CRISPR/CAS9突变体
    作者:Cynthia Denbow, Sonia Carole Ehivet and Sakiko Okumoto日期:07/20/2018,浏览量:3498,Q&A: 0
    [Abstract] CRISPR/Cas9 made targeted mutagenesis and genome editing possible for many plant species. One of the ways that the endonuclease is used for plant genetics is the creation of loss-of-function mutants, which typically result from erroneous DNA repair through non-homologous end joining (NHEJ) pathway. The majority of erroneous repair events results ...