植物科学

分类

    现刊
    Spatiotemporal Quantification of Cytosolic pH in Arabidopsis Pollen Tubes
    拟南芥花粉管胞质pH的时空定量研究
    作者:Maria Teresa Portes, Daniel S.C. Damineli and José A. Feijó日期:07/20/2021,浏览量:839,Q&A: 0
    [Abstract]

    Ion-specific probes and fluorescent indicators have been key in establishing the role of ion signaling, namely calcium, protons, and anions, in plant development, providing a robust approach for monitoring spatiotemporal changes in intracellular ion dynamics. The integration of protons/pH in signaling mechanisms is especially important as reports

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    Rice Root Hair Phenotypes Imaged by Cryo-SEM
    水稻根毛表型的低温扫描电镜成像
    [Abstract]

    Cryo-scanning electron microscopy (cryo-SEM) was first introduced for scientific use in the 1980s. Since then, cryo-SEM has become a routine technique for studying the surfaces and internal structures of biological samples with a high water content. In contrast to traditional SEM, cryo-SEM requires no sample pretreatment processes; thus, we can

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    Age, Wound Size and Position of Injury – Dependent Vascular Regeneration Assay in Growing Leaves
    年龄、伤口大小和损伤部位 – 生长叶片中依赖于损伤的维管再生试验
    [Abstract]

    Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf vasculature remain unexplored. Recently, we established a new vascular regeneration assay in

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    Histochemical Staining of Suberin in Plant Roots
    植物根部软木脂的组织化学染色
    作者:Peter Marhavý and Shahid Siddique日期:02/05/2021,浏览量:2178,Q&A: 0
    [Abstract]

    Histological stains are useful tools for characterizing cell shape, arrangement and the material they are made from. Stains can be used individually or simultaneously to mark different cell structures or polymers within the same cells, and to visualize them in different colors. Histological stains can be combined with genetically-encoded

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    Measuring Extracellular Proton and Anionic Fluxes in Arabidopsis Pollen Tubes
    拟南芥花粉管细胞外质子和阴离子通量的测定
    作者:Maria Teresa Portes and José A. Feijó日期:02/05/2021,浏览量:2178,Q&A: 0
    [Abstract]

    The ion-selective vibrating probe has been used to detect and quantify the magnitude and direction of transmembrane fluxes of several ions in a wide range of biological systems. Inherently non-invasive, vibrating probes have been essential to access relevant electrophysiological parameters related to apical growth and morphogenesis in pollen

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    Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa
    大麻种子快速高效发芽规程的建立与标准化
    作者:Aleksei Sorokin, Narendra Singh Yadav, Daniel Gaudet and Igor Kovalchuk日期:01/05/2021,浏览量:1818,Q&A: 0
    [Abstract]

    Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa using a hydrogen peroxide (H2O2) solution as liquid germination media. In this protocol, all three

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    A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia
    脉冲追踪EdU法检测拟南芥和笄石菖叶原基细胞分裂方向
    作者:Xiaofeng Yin and Hirokazu Tsukaya日期:01/05/2021,浏览量:1303,Q&A: 0
    [Abstract] In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are difficult. The few existing methods for detection and quantification of cell division orientation are ...
    Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes
    大分子直接导入水稻卵细胞和受精卵的基因表达和基因组编辑系统
    作者:Erika Toda and Takashi Okamoto日期:07/20/2020,浏览量:1712,Q&A: 0
    [Abstract] Polyethylene glycol calcium (PEG-Ca2+)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered into protoplasts derived from somatic tissues or calli via PEG-Ca2+ transfection. To ...
    Transient Expression Assay in Strawberry Fruits
    草莓果实中的瞬时表达检测
    作者:Mengting Pi, Qi Gao and Chunying Kang日期:06/05/2019,浏览量:4741,Q&A: 0
    [Abstract] Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria × ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions. In strawberry, ...
    Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy
    利用电子显微镜免疫金标记法进行植物细胞壁表位的可视化观察
    作者:Mateusz Majda日期:04/05/2019,浏览量:4361,Q&A: 0
    [Abstract] Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane. The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development. The visualization of cell wall components in ...