植物科学

分类

    现刊
    Proteoliposomes for Studying Lipid-protein Interactions in Membranes in vitro
    体外研究膜脂-蛋白相互作用的蛋白质脂质体
    作者:Helmut Kirchhoff日期:10/20/2021,浏览量:402,Q&A: 0
    [Abstract]

    Lipids in biomembranes can control the structure and, therefore, the functionality of membrane-embedded protein complexes. Unraveling how the lipid composition determines the mode of operation of membrane proteins provides mechanistic insights into their functionality. We applied a proteoliposome technique for studying how proteins function in

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    High-throughput Screening for Defense Priming-inducing Compounds in Parsley Cell Cultures
    欧芹细胞防御引物诱导化合物的高通量筛选
    作者:Kathrin Schmitz, Linda Werner and Uwe Conrath日期:10/20/2021,浏览量:392,Q&A: 0
    [Abstract]

    Defense priming describes the enhanced potency of cells to activate defense responses. Priming accompanies local and systemic immune responses and can be triggered by microbial infection or upon treatment with certain chemicals. Thus, chemically activating defense priming is promising for biomedicine and agriculture. However, test systems for

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    Dark Respiration Measurement from Arabidopsis Shoots
    拟南芥芽的暗呼吸测量
    作者:Jose P. Fonseca, Marcus Griffiths, Larry M. York and Kirankumar S. Mysore日期:10/05/2021,浏览量:953,Q&A: 0
    [Abstract]

    Dark respiration refers to experimental measures of leaf respiration in the absence of light, done to distinguish it from the photorespiration that occurs during photosynthesis. Dark aerobic respiration reactions occur solely in the mitochondria and convert glucose molecules from cytoplasmatic glycolysis and oxygen into carbon dioxide and water,

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    Tomato Stem Injection for the Precise Assessment of Ralstonia solanacearum Fitness in Planta
    番茄茎杆注射法精确评价青枯菌在植物中的适应性
    作者:Yaru Wang, Liu Xian, Gang Yu and Alberto P. Macho日期:08/20/2021,浏览量:773,Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a soil-borne pathogen with worldwide distribution that causes bacterial wilt disease in more than 250 plant species. R. solanacearum invades plants through the roots, reaches the vascular system, and colonizes the whole plant by moving through the xylem, where it eventually replicates rapidly, causing plant death. Usual

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    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    利用本生烟叶片基因瞬时表达或沉默研究青枯菌毒力的快速简便方法
    作者:Wenjia Yu and Alberto P. Macho日期:08/05/2021,浏览量:1161,Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed by observation and scoring of disease symptoms. Here, we describe a novel inoculation protocol to

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    Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS
    气质联用技术分析水稻组织中微量可溶性糖含量
    作者:Li-he Xu, Li-ying Xiao, Xue-qiong Xiao, Yan-nong Xiao and Gao-feng Wang日期:07/05/2021,浏览量:1734,Q&A: 0
    [Abstract]

    Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies have been developed to quantitate soluble sugar content in plant tissues; however, it is difficult

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    Micrografting in Arabidopsis Using a Silicone Chip
    利用硅芯片进行拟南芥微嫁接
    作者:Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi日期:06/20/2021,浏览量:2066,Q&A: 1
    [Abstract]

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including defense mechanisms and responses to surrounding environments such as soil and light conditions.

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    Measurements of Root Colonized Bacteria Species
    根定植细菌种类的测量
    作者:Vílchez Juan Ignacio, Yu Yang, Dian Yi and Huiming Zhang日期:04/05/2021,浏览量:2450,Q&A: 0
    [Abstract]

    Root-associated bacteria are able to influence plant fitness and vigor. A key step in understanding the belowground plant-bacteria interactions is to quantify root colonization by the bacteria of interest. Probably, genetic engineering with fluorescence markers is the most powerful way to monitor bacterial strains in plant. However, this could

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    Design and Construction of Repacked Soil Columns for Measuring Solute Transport, Plant Growth and Soil Biota
    用于测量溶质迁移、植物生长和土壤生物区系的重装土柱的设计和建造
    作者:Israel Ikoyi and Achim Schmalenberger日期:01/20/2021,浏览量:1426,Q&A: 0
    [Abstract]

    Researchers face a number of challenges in the construction of soil columns which can affect the outcome of their experiments. The use of intact soil cores closely mimics actual field conditions. However, the excavation of large intact soil cores is a time-consuming, labor-intensive process and may lead to soil compaction that would influence the

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    Pipecolic Acid Quantification Using Gas Chromatography-coupled Mass Spectrometry
    哌啶酸的气相色谱-质谱定量分析
    作者:Keshun Yu, Huazhen Liu and Pradeep Kachroo日期:12/05/2020,浏览量:1724,Q&A: 0
    [Abstract]

    Pipecolic acid (Pip), a non-proteinacious product of lysine catabolism, is an important regulator of immunity in plants and humans alike. For instance, Pip accumulation is associated with the genetic disorder Zellweger syndrome, chronic liver diseases, and pyridoxine-dependent epilepsy in humans. In plants, Pip accumulates upon pathogen infection

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