植物科学

分类

    现刊
    Tomato Stem Injection for the Precise Assessment of Ralstonia solanacearum Fitness in Planta
    番茄茎杆注射法精确评价青枯菌在植物中的适应性
    作者:Yaru Wang, Liu Xian, Gang Yu and Alberto P. Macho日期:08/20/2021,浏览量:444,Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a soil-borne pathogen with worldwide distribution that causes bacterial wilt disease in more than 250 plant species. R. solanacearum invades plants through the roots, reaches the vascular system, and colonizes the whole plant by moving through the xylem, where it eventually replicates rapidly, causing plant death. Usual

    ...
    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    利用本生烟叶片基因瞬时表达或沉默研究青枯菌毒力的快速简便方法
    作者:Wenjia Yu and Alberto P. Macho日期:08/05/2021,浏览量:802,Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed by observation and scoring of disease symptoms. Here, we describe a novel inoculation protocol to

    ...
    Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS
    气质联用技术分析水稻组织中微量可溶性糖含量
    作者:Li-he Xu, Li-ying Xiao, Xue-qiong Xiao, Yan-nong Xiao and Gao-feng Wang日期:07/05/2021,浏览量:1496,Q&A: 0
    [Abstract]

    Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies have been developed to quantitate soluble sugar content in plant tissues; however, it is difficult

    ...
    Micrografting in Arabidopsis Using a Silicone Chip
    利用硅芯片进行拟南芥微嫁接
    作者:Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi日期:06/20/2021,浏览量:1625,Q&A: 1
    [Abstract]

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including defense mechanisms and responses to surrounding environments such as soil and light conditions.

    ...
    Measurements of Root Colonized Bacteria Species
    根定植细菌种类的测量
    作者:Vílchez Juan Ignacio, Yu Yang, Dian Yi and Huiming Zhang日期:04/05/2021,浏览量:2160,Q&A: 0
    [Abstract]

    Root-associated bacteria are able to influence plant fitness and vigor. A key step in understanding the belowground plant-bacteria interactions is to quantify root colonization by the bacteria of interest. Probably, genetic engineering with fluorescence markers is the most powerful way to monitor bacterial strains in plant. However, this could

    ...
    Design and Construction of Repacked Soil Columns for Measuring Solute Transport, Plant Growth and Soil Biota
    用于测量溶质迁移、植物生长和土壤生物区系的重装土柱的设计和建造
    作者:Israel Ikoyi and Achim Schmalenberger日期:01/20/2021,浏览量:1220,Q&A: 0
    [Abstract]

    Researchers face a number of challenges in the construction of soil columns which can affect the outcome of their experiments. The use of intact soil cores closely mimics actual field conditions. However, the excavation of large intact soil cores is a time-consuming, labor-intensive process and may lead to soil compaction that would influence the

    ...
    Pipecolic Acid Quantification Using Gas Chromatography-coupled Mass Spectrometry
    哌啶酸的气相色谱-质谱定量分析
    作者:Keshun Yu, Huazhen Liu and Pradeep Kachroo日期:12/05/2020,浏览量:1529,Q&A: 0
    [Abstract]

    Pipecolic acid (Pip), a non-proteinacious product of lysine catabolism, is an important regulator of immunity in plants and humans alike. For instance, Pip accumulation is associated with the genetic disorder Zellweger syndrome, chronic liver diseases, and pyridoxine-dependent epilepsy in humans. In plants, Pip accumulates upon pathogen infection

    ...
    K+ Release Assay and K+ Measurement in Oocyte Assay
    卵母细胞K+释放量及K+水平测定
    作者:Hong Li日期:11/05/2020,浏览量:1420,Q&A: 0
    [Abstract] The Xenopus oocyte is a powerful system for the exogenous expression and functional characterization of plant membrane transport proteins. Until now, a number of potassium transporters and channels have been identified in oocytes expression system by the two-electrode voltage clamp technology. It is difficult to characterize K+ ...
    A Protocol for Flavonols, Kaempferol and Quercetin, Staining in Plant Root Tips
    植物根尖中黄酮醇、山奈酚和槲皮素的染色方法
    作者:Nguyen Hoai Nguyen日期:10/05/2020,浏览量:1829,Q&A: 0
    [Abstract] Flavonols are a subclass of flavonoids of the group of plant secondary metabolites. In planta, flavonols play various functions such as antioxidant and natural regulator of auxin polar transport. Many lines of evidence have shown that flavonols also contribute to human health in anti-oxidation, anti-inflammation, and even prevention some ...
    Expression and Purification of Arabidopsis Transmembrane Protein BCM1 in Saccharomyces cerevisiae
    拟南芥跨膜蛋白BCM1在酿酒酵母中的表达与纯化
    作者:Peng Wang and Bernhard Grimm日期:09/20/2020,浏览量:2007,Q&A: 0
    [Abstract] Heterologous expression and purification of transmembrane proteins have remained a challenge for decades hampering detailed biochemical and structural characterization of key enzymes and their interacting regulators in multiple metabolic pathways. An in-depth study on the newly identified Arabidopsis thaliana integral membrane protein ...