细胞生物学

分类

    现刊
    Enrichment of Vascular Fragments from Mouse Embryonic Brains for Endothelial Cell Analysis
    用于内皮细胞分析小鼠胚胎脑血管片段富集
    作者:Nicolas Santander and Thomas D. Arnold日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Endothelial cells in the brain interact with other cell types, forming the blood-brain barrier. This barrier controls the movement of solutes into and out of the brain, regulating pathophysiological processes and drug delivery to the brain. Common isolation methods used to study these cells during embryonic development involve enzymatic treatment

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    Quantitation of Secretory Granule Size in Drosophila Larval Salivary Glands
    果蝇幼虫唾液腺分泌颗粒大小的定量研究
    作者:Cheng-I J. Ma and Julie A. Brill日期:06/05/2021,浏览量:276,Q&A: 0
    [Abstract]

    Maturation of secretory granules is a crucial process that ensures the bioactivity of cargo proteins undergoing regulated secretion. In Drosophila melanogaster, the larval salivary glands produce secretory granules that are up to four-fold larger in cross-sectional area after maturation. Therefore, we developed a live imaging microscopy approach

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    Isolation of First-Trimester and Full-term Human Placental Hofbauer Cells
    妊娠早期和足月人胎盘霍夫鲍尔细胞的分离
    作者:Anna Appios, Jake R. Thomas and Naomi McGovern日期:06/05/2021,浏览量:754,Q&A: 0
    [Abstract]

    The placenta is the crucial organ that regulates the health of both mother and fetus during pregnancy. The human placenta is composed of villous tree-like structures that embed into the maternal decidua. Within the stroma of the villi resides a population of fetally-derived macrophages, the Hofbauer cells (HBC). HBC are the only fetal immune cells

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    Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions
    培养神经元细胞成细胞体和神经突部分的机械分离
    作者:Ankita Arora, Raeann Goering, Hei-Yong G. Lo and J. Matthew Taliaferro日期:06/05/2021,浏览量:606,Q&A: 0
    [Abstract]

    Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules that encode them. A key question in the study of this process of RNA localization is the

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    Generation and Maintenance of Homogeneous Human Midbrain Organoids
    同种人类中脑类器官的生成与维持
    作者:Henrik Renner, Martha Grabos, Hans R. Schöler and Jan M. Bruder日期:06/05/2021,浏览量:415,Q&A: 0
    [Abstract]

    Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a number of protocols for the generation of neural organoids have been published, most protocols require

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    FACS Enrichment of Total Interstitial Cells and Fibroblasts from Adult Mouse Ventricles
    流式细胞术富集成年小鼠心室间质细胞和成纤维细胞
    作者:Nona Farbehi, Vaibhao Janbandhu, Robert E. Nordon and Richard P. Harvey日期:05/20/2021,浏览量:966,Q&A: 0
    [Abstract]

    Besides cardiomyocytes, the heart contains numerous interstitial cell types, including cardiac fibroblasts, endothelial cells, immune (myeloid and lymphoid) cells, and mural cells (pericytes and vascular smooth muscle cells), which play key roles in heart repair, regeneration, and disease. We recently published a comprehensive map of cardiac

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    A Simple and Robust Protocol for in vitro Differentiation of Mouse Non-pathogenic T Helper 17 Cells from CD4+ T Cells
    一种简单稳定的用小鼠CD4+ T细胞体外分化非致病性T辅助17细胞的方法
    作者:Siwen Kang, Ruohan Wu and Ruoning Wang日期:05/20/2021,浏览量:735,Q&A: 0
    [Abstract]

    Functional and mechanistic studies of CD4+ T cell lineages rely on robust methods of in vitro T cell polarization. Here, we report an optimized protocol for in vitro differentiation of a mouse non-pathogenic T helper 17 (TH17) cell lineage. Most of the previously established protocols require irradiated splenocytes as artificial antigen presenting

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    Isolation of GFP-expressing Malarial Hypnozoites by Flow Cytometry Cell Sorting
    流式细胞术分离表达GFP的疟原虫催眠素
    作者:Annemarie Voorberg-van der Wel, Sam O. Hoffman and Clemens H. M. Kocken日期:05/05/2021,浏览量:341,Q&A: 0
    [Abstract]

    Hypnozoites are dormant liver-stage parasites unique to relapsing malarial species, including the important human pathogen Plasmodium vivax, and pose a barrier to the elimination of malaria. Little is known regarding the biology of these stages, largely due to their inaccessible location. Hypnozoites can be cultured in vitro but these cultures

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    A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
    一个健康的乳腺有机样系统来模拟哺乳和退化样过程
    作者:Elsa Charifou, Jakub Sumbal, Zuzana Koledova, Han Li and Aurélie Chiche日期:04/20/2021,浏览量:1100,Q&A: 0
    [Abstract]

    The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding

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    Measurements of Root Colonized Bacteria Species
    根定植细菌种类的测量
    作者:Vílchez Juan Ignacio, Yu Yang, Dian Yi and Huiming Zhang日期:04/05/2021,浏览量:921,Q&A: 0
    [Abstract]

    Root-associated bacteria are able to influence plant fitness and vigor. A key step in understanding the belowground plant-bacteria interactions is to quantify root colonization by the bacteria of interest. Probably, genetic engineering with fluorescence markers is the most powerful way to monitor bacterial strains in plant. However, this could

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