细胞生物学

分类

    现刊
    OrganoPlate Micro-fluidic Microvessel Culture and Analysis
    OrganoPlate微流控微血管培养与分析
    作者:Abidemi Junaid and Thomas Hankemeier日期:07/05/2021,浏览量:1451,Q&A: 0
    [Abstract]

    The endothelial cells from the microvasculature are key drivers and targets of inflammatory and thrombotic processes in microvascular diseases. The study of bioactive lipids in inflammatory processes has been largely based on two-dimensional endothelial cell cultures. Three-dimensional microvessels-on-a-chip provides an opportunity to monitor the

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    Intestinal Co-culture System to Study TGR5 Agonism and Gut Restriction
    利用肠道共培养系统研究TGR5激动剂和肠道限制性
    作者:Snehal N. Chaudhari and A. Sloan Devlin日期:03/20/2021,浏览量:2278,Q&A: 1
    [Abstract]

    The activation of the Takeda G-protein receptor 5 (TGR5, also known as the G protein-coupled bile acid receptor 1, GPBAR1) in enteroendocrine L-cells results in secretion of the anti-diabetic hormone Glucagon-Like Peptide 1 (GLP-1) into systemic circulation. Consequently, recent research has focused on identification and development of TGR5

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    Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders
    人来源iPSC获得的神经祖细胞(NPCs)的产生作为神经和线粒体疾病的药物发现模型
    作者:Annika Zink, Pawel Lisowski and Alessandro Prigione日期:03/05/2021,浏览量:2524,Q&A: 1
    [Abstract]

    The high attrition rate in drug development processes calls for additional human-based model systems. However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced pluripotent stem cells (iPSCs) has revolutionized the field of neuronal disease modeling and drug discovery.

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    Extraction and Quantification of Sphingolipids from Hemiptera Insects by Ultra-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry
    超高效液相色谱-串联质谱联用法提取和定量半翅目昆虫鞘脂质
    作者:Ni Wang, Xiaoxiao Shi, Chao Zhang, Wenwu Zhou and Zeng-Rong Zhu日期:02/20/2021,浏览量:2316,Q&A: 0
    [Abstract]

    Sphingolipids are major structural components of endomembranes and have also been described as an intracellular second messenger involved in various biological functions in all eukaryotes and a few prokaryotes. Ceramides (Cer), the central molecules of sphingolipids, have been depicted in cell growth arrest, cell differentiation, and apoptosis.

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    Immunoprecipitation of Acetyl-lysine and Western Blotting of Long-chain acyl-CoA Dehydrogenases and Beta-hydroxyacyl-CoA Dehydrogenase in Palmitic Acid Treated Human Renal Tubular Epithelial Cells
    棕榈酸治疗的人肾小管上皮细胞中乙酰赖氨酸的免疫沉淀和长链酰基辅酶A脱氢酶和β-羟酰基辅酶A脱氢酶的蛋白质印迹
    作者:Tingting Lv, Suwei Zhu, Yuan Ma, Hong Feng and Qiang Wan日期:09/20/2020,浏览量:2187,Q&A: 0
    [Abstract] As one of the main energy metabolism organs, kidney has been proved to have high energy requirements and are more inclined to fatty acid metabolism as the main energy source. Long-chain acyl-CoA dehydrogenases (LCAD) and beta-hydroxyacyl-CoA dehydrogenase (beta-HAD), key enzymes involved in fatty acid oxidation, has been identified as the ...
    Metabolomic and Lipidomic Analysis of Bone Marrow Derived Macrophages
    骨髓衍生巨噬细胞的代谢组学和脂质组学分析
    作者:Gretchen L. Seim, Steven V. John and Jing Fan日期:07/20/2020,浏览量:3491,Q&A: 0
    [Abstract] Macrophages are highly plastic immune cells that are capable of adopting a wide array of functional phenotypes in response to environmental stimuli. The changes in macrophage function are often supported and regulated by changes in cellular metabolism. Capturing a comprehensive picture of metabolism is vital for understanding the role of metabolic ...
    Observing Nutrient Gradients, Gene Expression and Growth Variation Using the "Yeast Machine" Microfluidic Device
    使用“酵母机”微流控设备观察营养梯度,基因表达和生长变异
    [Abstract] The natural environment of microbial cells like bacteria and yeast is often a complex community in which growth and internal organization reflect morphogenetic processes and interactions that are dependent on spatial position and time. While most of research is performed in simple homogeneous environments (e.g., bulk liquid cultures), ...
    Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis
    哺乳动物细胞脂筏的分离及其脂质组学分析
    作者:Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J. Meikle and Dmitri Sviridov日期:07/05/2020,浏览量:2466,Q&A: 0
    [Abstract] Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and ...
    Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces
    小鼠粪便中短链脂肪酸的气相色谱检测
    作者:Chaozheng Zhang, An Liu, Tianshuang Zhang, Yi Li and Hua Zhao日期:07/05/2020,浏览量:2436,Q&A: 0
    [Abstract] Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called short-chain fatty acids. SCFAs are closely related to various aspects of the human body, so more and ...
    Isolation of Tumor Cells Based on Their Distance from Blood Vessels
    基于与血管距离的肿瘤细胞分离
    作者:Saran Kumar, Husni Sharife, Tirzah Kreisel, Libat Bar-Lev, Myriam Grunewald and Eli Keshet日期:05/20/2020,浏览量:2632,Q&A: 0
    [Abstract] Differential exposure of tumor cells to microenvironmental cues greatly impacts cell phenotypes, raising a need for position based sorting of tumor cells amenable to multiple OMICs and functional analyses. One such key determinant of tumor heterogeneity in solid tumors is its vasculature. Proximity to blood vessels (BVs) profoundly affects tumor ...