神经科学

分类

    现刊
    Identification of Socially-activated Neurons
    社交激活神经元的识别
    作者:Mary L. Phillips and Lucas Pozzo-Miller日期:09/05/2020,浏览量:2809,Q&A: 0
    [Abstract] Determining the neuronal circuitry responsible for specific behaviors is a major focus in the field of neurobiology. Activity-dependent immediate early genes (IEGs), transcribed and translated shortly after neurons discharge action potentials, have been used extensively to either identify or gain genetic access to neurons and brain regions ...
    Examining Cocaine Conditioning Place Preference in Mice
    可卡因调节小鼠位置偏爱研究
    作者:MaryElizabeth J. Simkevich, Rianne R. Campbell and André O. White日期:04/20/2020,浏览量:2019,Q&A: 0
    [Abstract] A key component of combating substance use disorders is understanding the neural mechanisms that support drug reward. Tasks such as self-administration assess the reinforcing properties of a drug using a learned behavior but require numerous training sessions and surgery. In comparison, the conditioned place preference (CPP) task assesses reward ...
    The Mouse Gambling Task: Assessing Individual Decision-making Strategies in Mice
    小鼠博弈任务:个体决策策略评估
    作者:Elsa Pittaras, Arnaud Rabat and Sylvie Granon日期:01/05/2020,浏览量:2400,Q&A: 0
    [Abstract] Decision-making is a complex cognitive process which consists of choosing one option among several alternatives. In humans, this process is featured in the Iowa gambling task (IGT), a decision-making task that mimics real life situations by reproducing uncertain conditions based on probabilistic rewards or penalties (see Background). Several ...
    Construction of Viral Vectors for Cell Type-specific CRISPR Gene Editing in the Adult Mouse Brain
    用于成年小鼠脑组织的细胞特异性CRISPR基因编辑的病毒载体的构建
    作者:Hiroshi Yamaguchi and Luis de Lecea日期:08/20/2019,浏览量:3326,Q&A: 0
    [Abstract] Recently developed gene editing technologies based on engineered CRISPR/Cas9 systems enables researchers to disrupt genes in a cell type-specific manner in the adult mouse brain. Using these technologies, we recently showed that the dopamine beta-hydroxylase gene in Locus Coeruleus (LC) norepinephrine neurons plays a vital role in the maintenance ...
    Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
    利用 Fast Passive CLARITY技术研究斑马鱼、啮齿动物、灵长类动物和人类的大脑
    作者:Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova日期:08/05/2019,浏览量:5402,Q&A: 0
    [Abstract] Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and stabilizing the cellular and subcellular structures. This provides effective penetration of diverse ...
    Accessing Olfactory Habituation in Drosophila melanogaster with a T-maze Paradigm
    使用T迷宫范例模拟黑腹果蝇的嗅觉习性
    作者:Ourania Semelidou, Summer F. Acevedo and Efthimios M.C. Skoulakis日期:06/05/2019,浏览量:3761,Q&A: 0
    [Abstract] Habituation is the process whereby perceptual changes alter the value of environmental stimuli, enabling salience filtering. This behavioral response decrement is a form of non-associative learning, where the subject learns about the stimulus and does not involve sensory adaptation, sensory or motor fatigue. The range of behavioral responses in D. ...
    High-resolution Immunoelectron Microscopy Techniques for Revealing Distinct Subcellular Type 1 Cannabinoid Receptor Domains in Brain
    检测脑中不同亚细胞1型大麻素受体结构域的高分辨率免疫电镜技术
    作者:Nagore Puente, Itziar Bonilla-Del Río, Svein Achicallende, Patrick C. Nahirney and Pedro Grandes日期:01/20/2019,浏览量:5469,Q&A: 0
    [Abstract] Activation of type 1 cannabinoid (CB1) receptors by endogenous, exogenous (cannabis derivatives) or synthetic cannabinoids (i.e., CP 55.940, Win-2) has a wide variety of behavioral effects due to the presence of CB1 receptors in the brain. In situ hybridization and immunohistochemical techniques have been ...
    Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2
    利用SeeD2培养基对组织样品光透明及折射率匹配以实现高分辨率成像
    作者:Meng-Tsen Ke and Takeshi Imai日期:10/20/2018,浏览量:7694,Q&A: 1
    [Abstract] Tissue clearing techniques are useful for large-scale three-dimensional fluorescence imaging of thick tissues. However, high-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we present a water-based optical clearing and mounting media, SeeDB2, which is ...
    Artificial Inhalation Protocol in Adult Mice
    成年小鼠人工吸气方法
    作者:Thomas P. Eiting and Matt Wachowiak日期:09/20/2018,浏览量:3395,Q&A: 0
    [Abstract] Research in the area of in vivo olfactory physiology benefits from having direct access to the nasal airways through which odorants can be presented. Ordinarily, the passage of odorants through the airways is controlled by respiratory rhythm. This fact makes it difficult to control the timing and strength of an olfactory stimulus, since ...
    Quantitative Electron Microscopic Assay Using Random Sampling from Single Sections to Test Plastic Synaptic Changes in Hippocampus
    单切片随机取样定量电子显微技术检测海马区突触可塑性的变化
    作者:G. Mark Marcello, Lilla E. Szabó, Peter Sotonyi and Bence Rácz日期:08/05/2018,浏览量:4017,Q&A: 0
    [Abstract] Studies over several decades on the organization of the CA1 hippocampus–a particularly favorable model for learning, memory and certain forms of cognition–have shown that the synaptic network in this brain region is plastic (Fortin et al., 2012). Recent evidence suggests that a number of environmental and endogenous stimuli may have a ...