微生物学

分类

    现刊
    Sex-specific Separation of Plasmodium falciparum Gametocyte Populations
    恶性疟原虫配子细胞群体的性别特异性分离
    作者:Melanie C. Ridgway, Daniela Cihalova and Alexander G. Maier日期:06/05/2021,浏览量:1264,Q&A: 0
    [Abstract]

    Plasmodium falciparum is a unicellular eukaryotic parasite that causes malaria in humans. The parasite is spread by Anopheles mosquitoes after ingestion of sexual stage parasites known as gametocytes. Malaria transmission depends on parasites switching from the disease-causing asexual blood forms to male and female gametocytes. The current

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    ODELAM: Rapid Sequence-independent Detection of Drug Resistance in Mycobacterium tuberculosis Isolates
    ODELAM:结核分枝杆菌耐药性的快速序列独立检测
    作者:Thurston Herricks, Magdalena Donczew, David R. Sherman and John D. Aitchison日期:05/20/2021,浏览量:1801,Q&A: 0
    [Abstract]

    Antimicrobial-resistant Mycobacterium tuberculosis (Mtb) causes over 200,000 deaths globally each year. Current assays of antimicrobial resistance require knowledge of the mutations that confer drug resistance or long periods of culture time to test growth under drug pressure. We present ODELAM (One-cell Doubling Evaluation of Living Arrays of

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    Screening for Lysogen Activity in Therapeutically Relevant Bacteriophages
    筛选与治疗相关的噬菌体的溶原活性
    作者:Fernando L. Gordillo Altamirano and Jeremy J. Barr日期:04/20/2021,浏览量:3030,Q&A: 1
    [Abstract]

    Lysogenic phages can integrate into their bacterial host’s genome, potentially transferring any genetic information they possess including virulence or resistance genes, and are therefore routinely excluded from therapeutic applications. Lysogenic behavior is typically seen in phages that create turbid plaques or possess subpar bactericidal

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    Primer ID Next-Generation Sequencing for the Analysis of a Broad Spectrum Antiviral Induced Transition Mutations and Errors Rates in a Coronavirus Genome
    用于分析冠状病毒基因组中广谱抗病毒药物诱导的过渡突变和错误率的引物ID下一代测序
    作者:Shuntai Zhou, Collin S. Hill, Michael U. Clark, Timothy P. Sheahan, Ralph Baric and Ronald Swanstrom日期:03/05/2021,浏览量:3301,Q&A: 0
    [Abstract]

    Next generations sequencing (NGS) has become an important tool in biomedical research. The Primer ID approach combined with the MiSeq platform overcomes the limitation of PCR errors and reveals the true sampling depth of population sequencing, making it an ideal tool to study mutagenic effects of potential broad-spectrum antivirals on RNA viruses.

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    Determination of Antibacterial Activity of Film Coatings against Four Clinically Relevant Bacterial Strains
    薄膜敷层对4种与临床相关菌株的抑菌活性测定
    [Abstract]

    Antibacterial coatings have currently gained great importance in biomedical technology investigations. Because of the spatial arrangement of the film coatings, evaluation of antibacterial activity presents a new challenge regarding traditional bacterial counting methods. In this protocol, four clinically relevant pathogens, Salmonella typhimurium,

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    Evaluation of Toxicity with Brine Shrimp Assay
    通过卤虫实验进行毒性评价
    作者:Christina N. Banti and Sotiris K. Hadjikakou日期:01/20/2021,浏览量:2574,Q&A: 0
    [Abstract]

    The in vivo toxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) or their hydrogels such as with hydroxyethyl-methacrylate (HEMA) (pHEMA@SBAMs or pHEMA@CoMeDs) are evaluated by the brine shrimp assay. Thus individuals of Artemia salina larvae are incubated in saline solutions with

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    Antimicrobial Sensitivity Assay for Bdellovibrio bacteriovorus
    噬菌蛭弧菌的药敏试验
    作者:Emanuele Marine and Klaas M. Pos日期:12/20/2020,浏览量:1303,Q&A: 0
    [Abstract]

    Bdellovibrio bacteriovorus, an obligate predatory bacterium [i.e., bacteria that kill and feed on other bacteria (prey)], has the potential to be used as a probiotic for the disinfection of surfaces or for the treatment of bacterial infections. One option is to use this organism in combination with antimicrobials to potentiate the effectiveness of

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    Rapid Isolation and Purification of Secreted Bacteriocins from Streptococcus mutans and Other Lactic Acid Bacteria
    变形链球菌及其他乳酸菌分泌细菌素的快速分离纯化
    作者:Marcus Cheng, Siew-Ging Gong and Céline M. Lévesque日期:11/20/2020,浏览量:2109,Q&A: 0
    [Abstract] Bacteriocins are small ribosomally synthesized antimicrobial peptides produced by some microorganisms including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for low pH. Bacteriocins kill bacteria rapidly and are biologically active at very low concentrations. Bacteriocins produced by LAB ...
    Construction and Validation of A Low-cost, Small-scale, Multiplex Continuous Culturing System for Microorganisms
    低成本、小规模、复合微生物连续培养系统的构建与验证
    作者:Lilit Tonoyan, Freddy Guihéneuf, Ruari Friel and Vincent O’Flaherty日期:11/05/2020,浏览量:1347,Q&A: 0
    [Abstract]

    This protocol details the construction of a simple, low-cost, small-scale, multiplex chemostat system designed for the continuous cultivation of microorganisms in suspension (i.e., bacteria, yeast, microalgae). The continuous culture device can operate at a working volume of 25 ml and allows the run of 8 chemostats in parallel by a single person.

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    Neutrophil Extracellular Trap Killing Assay of Candida albicans
    白念珠菌中性粒细胞胞外诱杀试验
    作者:Sheng-Yang Wu and Betty A. Wu-Hsieh日期:08/20/2020,浏览量:2472,Q&A: 0
    [Abstract] Fungal pathogen Candida albicans is one of the top leading causes of overall healthcare-associated bloodstream infections worldwide. Neutrophil is the major effector cell to clear C. albicans infection. Our study showed that mouse neutrophils utilize two independent mechanisms to kill C. albicans: one is CR3 downstream ...