系统生物学

分类

    现刊
    Low- and High-resolution Dynamic Analyses for Magnetic Resonance Spectroscopy Data
    磁共振光谱数据的低高分辨率动态分析
    作者:Reuben Rideaux日期:01/20/2021,浏览量:1541,Q&A: 0
    [Abstract]

    Magnetic resonance spectroscopy (MRS) can be used to measure in vivo concentrations of neurometabolites. This information can be used to identify neurotransmitter involvement in healthy (e.g., perceptual and cognitive processes) and unhealthy brain function (e.g., neurological and psychiatric illnesses). The standard approach for analyzing MRS

    ...
    Metabolomic and Lipidomic Analysis of Bone Marrow Derived Macrophages
    骨髓衍生巨噬细胞的代谢组学和脂质组学分析
    作者:Gretchen L. Seim, Steven V. John and Jing Fan日期:07/20/2020,浏览量:3658,Q&A: 0
    [Abstract] Macrophages are highly plastic immune cells that are capable of adopting a wide array of functional phenotypes in response to environmental stimuli. The changes in macrophage function are often supported and regulated by changes in cellular metabolism. Capturing a comprehensive picture of metabolism is vital for understanding the role of metabolic ...
    Experiments for in silico evaluation of Optimality of Photosynthetic Nitrogen Distribution and Partitioning in the Canopy: an Example Using Greenhouse Cucumber Plants
    树冠中光合氮分配的最适生物信息学评估实验:温室黄瓜应用实例
    作者:Yi-Chen Pao, Tsu-Wei Chen, Dany Pascal Moualeu-Ngangue and Hartmut Stützel日期:03/20/2020,浏览量:3308,Q&A: 0
    [Abstract] Acclimation of leaf traits to fluctuating environments is a key mechanism to maximize fitness. One of the most important strategies in acclimation to changing light is to maintain efficient utilization of nitrogen in the photosynthetic apparatus by continuous modifications of between-leaf distribution along the canopy depth and within-leaf ...
    Isolation and Quantification of Metabolite Levels in Murine Tumor Interstitial Fluid by LC/MS
    LC/MS法对小鼠肿瘤间质液中代谢产物的分离和定量
    作者:Mark R Sullivan, Caroline A Lewis and Alexander Muir日期:11/20/2019,浏览量:4926,Q&A: 0
    [Abstract] Cancer is a disease characterized by altered metabolism, and there has been renewed interest in understanding the metabolism of tumors. Even though nutrient availability is a critical determinant of tumor metabolism, there has been little systematic study of the nutrients directly available to cancer cells in the tumor microenvironment. Previous ...
    Non-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments of Arabidopsis Leaf Tissues
    非水体系分离(NAF)用于拟南芥叶组织亚细胞组分的代谢研究
    作者:David B. Medeiros, Stéphanie Arrivault, Jessica Alpers, Alisdair R. Fernie and Fayezeh Arabi日期:10/20/2019,浏览量:2670,Q&A: 0
    [Abstract] The accurate determination of metabolite distribution in subcellular compartments is still challenging in plant science. Various methodologies, such as fluorescence resonance energy transfer-based technology, nuclear magnetic resonance spectroscopy and protoplast fractionation allow the study of metabolite compartmentation. However, large changes ...
    Use of Gas Chromatography to Quantify Short Chain Fatty Acids in the Serum, Colonic Luminal Content and Feces of Mice
    利用气相色谱法定量分析小鼠血清、结肠管腔内容物和粪便中短链脂肪酸
    [Abstract] Short-Chain Fatty Acids (SCFAs) are a product of the fermentation of resistant starches and dietary fibers by the gut microbiota. The most important SCFA are acetate (C2), propionate (C3) and butyrate (C4). These metabolites are formed and absorbed in the colon and then transported through the hepatic vein to the liver. SCFAs are more concentrated ...
    Stable-isotope Labeled Metabolic Analysis in Drosophila melanogaster: From Experimental Setup to Data Analysis
    黑腹果蝇的稳定同位素标记代谢分析:从实验建立到数据分析
    作者:Yuping Cai, Nan Liu and Zheng-Jiang Zhu日期:09/20/2018,浏览量:4548,Q&A: 0
    [Abstract] Stable-isotope labeled metabolic analysis is an essential methodology to characterize metabolic regulation during biological processes. However, the method using stable-isotope-labeled tracer (e.g., 13C-glucose) in live animal is only beginning to be developed. Here, we contribute a qualitative metabolic labeling experiment ...
    Extraction and Analysis of Pan-metabolome Polar Metabolites by Ultra Performance Liquid Chromatography–Tandem Mass Spectrometry (UPLC-MS/MS)
    超高效液相色谱 - 串联质谱法(UPLC-MS/MS)提取和分析泛代谢组极性代谢产物
    作者:Dania M. Malik, Seth Rhoades and Aalim Weljie日期:02/05/2018,浏览量:6207,Q&A: 0
    [Abstract] Modern triple quadrupole mass spectrometers provide the ability to detect and quantify a large number of metabolites using tandem mass spectrometry (MS/MS). Liquid chromatography (LC) is advantageous, as it does not require derivatization procedures and a large diversity in physiochemical characteristics of analytes can be accommodated through a ...
    Identification and Quantification of Secondary Metabolites by LC-MS from Plant-associated Pseudomonas aurantiaca and Pseudomonas chlororaphis
    采用LC-MS法鉴定和定量植物相关桔黄假单胞菌和绿针铜绿假单胞菌中次级代谢产物
    作者:Izzah Shahid, Muhammad Rizwan and Samina Mehnaz日期:01/20/2018,浏览量:9243,Q&A: 0
    [Abstract] Increased antibiotic resistance of plants and human pathogens and continuous use of chemical fertilizers has pushed microbiologists to explore new microbial sources as potential antagonists. In this study, eight strains of Pseudomonas aurantiaca and Pseudomonas chlororaphis, have been isolated from different plant sources and ...
    Lipidomic Analysis of Caenorhabditis elegans Embryos
    秀丽隐杆线虫胚胎的脂质组学分析
    作者:Hung-Chi Yang, Cheng-Yu Hung, Yi-Yun Pan, Szecheng J Lo and Daniel Tsun-Yee Chiu日期:09/20/2017,浏览量:7614,Q&A: 0
    [Abstract] Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile animal model for novel discovery of lipid metabolism. In addition, C. elegans embryo is ...