细胞生物学

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    现刊
    APEX-mediated Proximity Labeling of Proteins in Cells Targeted by Extracellular Vesicles
    APEX 介导的细胞外囊泡靶向细胞中蛋白质的邻近标记
    作者:Lu Song, Jun Chen, Angela Sun and Randy Schekman日期:11/05/2021,浏览量:953,Q&A: 0
    [Abstract]

    Extracellular vesicles (EVs) are thought to mediate intercellular communication through the delivery of cargo proteins and RNA to target cells. The uptake of EVs is often followed visually using lipophilic-dyes or fluorescently-tagged proteins to label membrane constituents that are then internalized into recipient cells (Christianson et al.,

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    Cytoduction and Plasmiduction in Yeast
    酵母细胞诱导中的细胞诱导和质粒诱导
    作者:Jane E. Dorweiler and Anita L. Manogaran日期:09/05/2021,浏览量:1028,Q&A: 0
    [Abstract]

    Cytoduction, and a related technique referred to as plasmiduction, have facilitated substantial advancements in the field of yeast prion biology by providing a streamlined method of transferring prions from one yeast strain to another. Prions are cytoplasmic elements consisting of aggregated misfolded proteins, and as such, they exhibit

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    CRISPR/Cas9-mediated Precise SNP Editing in Human iPSC Lines
    CRISPR/Cas9介导的人iPSC细胞系SNP精确编辑
    作者:Hanwen Zhang and Siwei Zhang日期:06/20/2021,浏览量:1870,Q&A: 0
    [Abstract]

    Human induced pluripotent stem cells (hiPSCs) have been extensively used in the fields of developmental biology and disease modeling. CRISPR/Cas9 gene editing in iPSC lines often has a low frequency, which hampers its application in precise allele editing of disease-associated single nucleotide polymorphisms (SNPs), especially those in the

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    Production of Phenotypically Uniform Human Cerebral Organoids from Pluripotent Stem Cells
    从多能干细胞生产表型一致的人类大脑类器官
    作者:Adam Sivitilli, Parisa Ghiasi and Liliana Attisano日期:04/20/2021,浏览量:2504,Q&A: 0
    [Abstract]

    Recent advances in stem cell technology have allowed researchers to generate 3D cerebral organoids (COs) from human pluripotent stem cells (hPSCs). Indeed, COs have provided an unprecedented opportunity to model the developing human brain in a 3D context, and in turn, are suitable for addressing complex neurological questions by leveraging

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    Derivation of Induced Pluripotent Stem Cells from Human Fibroblasts Using a Non-integrative System in Feeder-free Conditions
    无饲养层条件下利用非整合系统从人成纤维细胞中衍生诱导多能干细胞
    作者:Alvaro A. Beltran, Sarahi G. Molina and Adriana S. Beltran日期:10/20/2020,浏览量:2496,Q&A: 0
    [Abstract] Induced pluripotent stem cells (iPSCs) are genetically reprogrammed somatic cells that exhibit features identical to those of embryonic stem cells (ESCs). Multiple approaches are available to derive iPSCs, among which the Sendai virus is the most effective at reprogramming different cell types. Here we describe a rapid, efficient, safe, and ...
    In vitro Differentiation of Human iPSC-derived Cardiovascular Progenitor Cells (iPSC-CVPCs)
    人iPSC衍生心血管祖细胞(iPSC-CVPCs)的体外分化
    作者:Agnieszka D’Antonio-Chronowska, Matteo D’Antonio and Kelly A. Frazer日期:09/20/2020,浏览量:4092,Q&A: 0
    [Abstract] Induced pluripotent stem cell derived cardiovascular progenitor cells (iPSC-CVPCs) provide an unprecedented platform for examining the molecular underpinnings of cardiac development and disease etiology, but also have great potential to play pivotal roles in the future of regenerative medicine and pharmacogenomic studies. Biobanks like iPSCORE ( ...
    Direct Reprogramming of Mouse Embryonic Fibroblasts to Conventional Type 1 Dendritic Cells by Enforced Expression of Transcription Factors
    转录因子强化表达直接将小鼠胚胎成纤维细胞重编程为常规1型树突状细胞
    作者:Fábio F. Rosa, Cristiana F. Pires, Olga Zimmermannova and Carlos-Filipe Pereira日期:05/20/2020,浏览量:3497,Q&A: 0
    [Abstract] Ectopic expression of transcription factor combinations has been recently demonstrated to reprogram differentiated somatic cells towards the dendritic cell (DC) lineage without reversion to a multipotent state. DCs have the ability to induce potent and long-lasting adaptive immune responses. In particular, conventional type 1 DCs (cDC1s) excel on ...
    Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies
    快速建立能诱导表达功能研究所需蛋白的人神经细胞模型
    [Abstract] CRISPR-Cas9 technology has transformed the ability to edit genomic sequences and control gene expression with unprecedented ease and scale. However, precise genomic insertions of coding sequences using this technology remain time-consuming and inefficient because they require introducing adjacent single-strand cuts through Cas9 nickase action and ...
    CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
    诺氏疟原虫CRISPR-Cas9基因编辑
    作者:Franziska Mohring, Melissa N. Hart, Avnish Patel, David A. Baker and Robert W. Moon日期:02/20/2020,浏览量:3592,Q&A: 1
    [Abstract] Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. falciparum, P. knowlesi is the only other malaria parasite capable of being maintained in ...
    In vitro Self-organized Mouse Small Intestinal Epithelial Monolayer Protocol
    小鼠小肠上皮单层膜体外自组装实验方案
    作者:Gizem Altay, Eduard Batlle, Vanesa Fernández-Majada and Elena Martinez日期:02/05/2020,浏览量:6473,Q&A: 0
    [Abstract] Developing protocols to obtain intestinal epithelial monolayers that recapitulate in vivo physiology to overcome the limitations of the organoids’ closed geometry has become of great interest during the last few years. Most of the developed culture models showed physiological-relevant cell composition but did not prove self-renewing ...