微生物学

分类

    现刊
    Glucose Starvation, Magnesium Ion Starvation, and Bile Stress Assays
    葡萄糖饥饿、镁离子饥饿和胆汁应激分析
    作者:Aryashree Arunima and Mrutyunjay Suar日期:09/20/2021,浏览量:419,Q&A: 0
    [Abstract]

    Salmonella enterica serovar Enteritidis (S. Enteritidis) is a leading causative pathogen for food-borne gastroenteritis. During its course of infection, it confronts myriads of physiological barriers inside the host, such as nutrient deprivation, low micronutrient availability, and toxicity from bile salts, to promote bacterial survival and

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    Acid Hydrolysis for the Extraction of Archaeal Core Lipids and HPLC-MS Analysis
    酸水解法提取古生菌核心脂质及HPLC-MS分析
    作者:Maxime Tourte, Philippe Schaeffer, Vincent Grossi and Philippe M. Oger日期:08/20/2021,浏览量:459,Q&A: 0
    [Abstract]

    Lipid membranes are essential cellular elements as they provide cellular integrity and selective permeability under a broad range of environmental settings upon cell growth. In particular, Archaea are commonly recognized for their tolerance to extreme conditions, which is now widely accepted to stem from the unique structure of their lipids. While

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    Ion Transport Activity Assay for Microbial Rhodopsin Expressed in Escherichia coli Cells
    大肠杆菌表达的微生物紫红质离子转运活性测定
    作者:Masae Konno, Keiichi Inoue and Hideki Kandori日期:08/05/2021,浏览量:763,Q&A: 0
    [Abstract]

    Microbial rhodopsins have diverse functions, including roles as light-driven ion pumps, light-gated ion channels, photosensors, and light-regulated enzymes. As the number of rhodopsin-like genes identified has increased in recent years, so has the requirement for rapid identification of their functions. The patch-clamp method is often used to

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    Fluorescence-based Heme Quantitation in Toxoplasma Gondii
    基于荧光的弓形虫血红素定量
    作者:Amy Bergmann and Zhicheng Dou日期:06/20/2021,浏览量:1167,Q&A: 0
    [Abstract]

    Toxoplasma gondii is a highly prevalent protozoan pathogen throughout the world. As a eukaryotic intracellular pathogen, Toxoplasma ingests nutrients from host cells to support its intracellular growth. The parasites also encode full or partial metabolic pathways for the biosynthesis of certain nutrients, such as heme. Heme is an essential

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    ODELAM: Rapid Sequence-independent Detection of Drug Resistance in Mycobacterium tuberculosis Isolates
    ODELAM:结核分枝杆菌耐药性的快速序列独立检测
    作者:Thurston Herricks, Magdalena Donczew, David R. Sherman and John D. Aitchison日期:05/20/2021,浏览量:1911,Q&A: 0
    [Abstract]

    Antimicrobial-resistant Mycobacterium tuberculosis (Mtb) causes over 200,000 deaths globally each year. Current assays of antimicrobial resistance require knowledge of the mutations that confer drug resistance or long periods of culture time to test growth under drug pressure. We present ODELAM (One-cell Doubling Evaluation of Living Arrays of

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    High-throughput Method for Detecting Siderophore Production by Rhizosphere Bacteria
    根际细菌产生铁载体的高通量检测方法
    作者:ShaoHua Gu, Wen Wan, ZhengYing Shao and Wei Zhong日期:05/05/2021,浏览量:1936,Q&A: 0
    [Abstract]

    Siderophores, a key substance that microorganisms produce to obtain iron under iron-limited conditions, play an important role in regulating interactions between beneficial bacteria and pathogenic bacteria. A large number of bacteria were isolated from the rhizosphere, and we used the method presented here to assay the siderophore production by

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    Bacterial Conjugation Protocol for Ruminant Mycoplasmas
    反刍动物支原体细菌接合的实验方法
    作者:Eveline Sagné, Christine Citti and Emilie Dordet-Frisoni日期:01/20/2021,浏览量:1075,Q&A: 0
    [Abstract]

    In Mycoplasma agalactiae, two simultaneous processes of DNA transfer have been described that require direct cell-to-cell contact and are similar to conjugation. One involves the self-transmission of an integrative conjugative element (ICE) while the second concerns the horizontal transfer of large and small fragments of chromosomal DNA. Here, we

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    Charging State Analysis of Transfer RNA from an α-proteobacterium
    α-变形杆菌转运RNA的装载状态分析
    作者:Liang Yin and Caroline S. Harwood日期:12/05/2020,浏览量:1136,Q&A: 0
    [Abstract] Transfer RNA (tRNA) is an essential link between the genetic code and proteins. During the process of translation, tRNA is charged with its cognate amino acid and delivers it to the ribosome, thus serving as a substrate of protein synthesis. To analyze the charging state of a particular tRNA, total RNA is purified and analyzed on an acid-urea gel. ...
    Ribosome Purification from an α-proteobacterium and rRNA Analysis by Northern Blot
    α-变形杆菌核糖体的纯化及Northern印迹分析
    作者:Liang Yin and Caroline S. Harwood日期:12/05/2020,浏览量:1107,Q&A: 0
    [Abstract] Ribosomes are an integral part of cellular life. They are complex molecular machines consisting of multiple ribosomal proteins and RNAs. To study different aspects of ribosome composition, many methods have been developed over the decades. Here, we describe how to purify ribosomes from the α-proteobacterium Rhodopseudomonas palustris ...
    A High-throughput Interbacterial Competition Platform
    一个高通量细菌间竞争平台
    作者:Hsiao-Han Lin and Erh-Min Lai日期:09/05/2020,浏览量:2225,Q&A: 0
    [Abstract] Contact-dependent interbacterial competition is a common strategy used by bacteria to fight for their ecological niches. Interbacterial competition is monitored by a competition assay involving co-culturing the attacker and the recipient bacterial cells on agar, followed by recovery of the surviving recipient cells. Conventional interbacterial ...