癌症生物学

分类

    现刊
    Isolation of Murine Primary Aortic Smooth Muscle Cells
    小鼠主动脉平滑肌细胞的分离
    作者:Max Ole Hubert, Juan Rodriguez-Vita, Lena Wiedmann and Andreas Fischer日期:02/05/2021,浏览量:1967,Q&A: 0
    [Abstract]

    Vascular smooth muscle cells (VSMCs) have been cultured for decades to study the role of these cells in cardiovascular disorders. The most common source of VSMCs is the rat aorta. Here we show the adaptation of this method to isolate and culture mouse aortic VSMCs. The advantage of this method is that there are many more transgenic mouse lines

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    Imaging the Vasculature of Immunodeficient Mice Using Positron Emission Tomography/Computed Tomography (PET/CT) and 18F-fluorodeoxyglucose Labeled Human Erythrocytes
    利用正电子发射断层扫描/计算机断层扫描(PET、CT)和18F-氟脱氧葡萄糖标记人类红细胞 进行免疫缺陷小鼠脉管系统成像
    作者:Shaowei Wang and Jung W. Choi日期:10/05/2019,浏览量:2424,Q&A: 0
    [Abstract] Nuclear blood pool imaging using radiolabeled red blood cells has been used in the clinical setting for the evaluation of a number of medical conditions including gastrointestinal hemorrhage, impaired cardiac contractility, and altered cerebrovascular blood flow. Nuclear blood pool imaging is typically performed using Technetium-99m-labeled (99m ...
    Human Endothelial Cell Spheroid-based Sprouting Angiogenesis Assay in Collagen
    在胶原蛋白中分析人内皮细胞基于球体的出芽式血管生成
    作者:Fabian Tetzlaff and Andreas Fischer日期:09/05/2018,浏览量:11991,Q&A: 0
    [Abstract] Angiogenesis, the formation of new blood vessels from pre-existing ones plays an important role during organ development, regeneration and tumor progression. The spheroid-based sprouting assay is a well-established and robust method to study the influence of genetic alterations or pharmacological compounds on capillary-like tube formation of ...
    Evaluation of Angiogenesis Inhibitors Using the HUVEC Fibrin Bead Sprouting Assay
    采用HUVEC纤维蛋白珠催芽试验评估血管生成抑制因子
    作者:Laura Winters, Nithya Thambi, Julian Andreev and Frank Kuhnert日期:10/05/2016,浏览量:11168,Q&A: 0
    [Abstract] Angiogenesis, the growth of new blood vessels from pre-existing vessels, is a critical process that occurs during normal development and tumor formation. Targeting tumor angiogenesis by blocking the activity of vascular endothelial growth factor (VEGF) has demonstrated some clinical benefit; nevertheless there is a great need to target additional ...
    Rat Aortic Ring Model to Assay Angiogenesis ex vivo
    利用大鼠主动脉环的体外活体模型测定血管生成
    作者:Isabelle Ernens, Bénédicte Lenoir, Yvan Devaux and Daniel R. Wagner日期:10/20/2015,浏览量:8808,Q&A: 0
    [Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These assays mainly use monolayers of ...
    In vivo Chick Chorioallantoic Membrane (CAM) Angiogenesis Assays
    雏鸡绒毛尿囊膜(CAM)体内血管形成实验
    作者:Zhenguo Chen, Zhihua Wen and Xiaochun Bai日期:09/20/2013,浏览量:34076,Q&A: 3
    [Abstract] Angiogenesis is the process of formation of new blood vessels from pre-existing vessels or endothelial cell progenitors. It plays a crucial role in tumor growth and metastasis. Tumor angiogenesis have been widely studied as an important target for suppressing tumor growth and metastasis. Here, we describe an in vivo chick embryo ...
    In vitro Human Umbilical Vein Endothelial Cells (HUVEC) Tube-formation Assay
    人脐静脉血管内皮细胞(HUVEC)体外成管实验
    作者:Josephine MY Ko and Maria Li Lung日期:09/20/2012,浏览量:60386,Q&A: 1
    [Abstract] Angiogenesis is involved not only in pathological conditions including cancer biology and non-neoplastic diseases, but also many biological processes including reproduction, development and repair. During angiogenesis, endothelial cells (ECs) undergo activation after binding of angiogenic factors to their receptors, release of proteases to ...
    In vivo Matrigel Plug Angiogenesis Assay
    基质胶内血管生成实验
    作者:Hong Lok Lung and Maria Li Lung日期:09/20/2012,浏览量:45892,Q&A: 4
    [Abstract] The matrigel plug angiogenesis assay is a simple in vivo technique to detect the newly formed blood vessels in the transplanted gel plugs in nude mice. The matrigel matrix is derived from the engelbroth-holm-swarm (EHS) mouse sarcoma, and its composition is comparable to the basement membrane proteins. The matrigel can induce ...