免疫学

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    现刊
    An Image-based Dynamic High-throughput Analysis of Adherent Cell Migration
    一种基于图像的动态高通量分析黏附细胞的迁移
    [Abstract]

    In this protocol, we describe a method to monitor cell migration by live-cell imaging of adherent cells. Scratching assay is a common method to investigate cell migration or wound healing capacity. However, achieving homogenous scratching, finding the optimal time window for end-point analysis and performing an objective image analysis imply, even

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    An Imaging Flow Cytometry Method to Measure Citrullination of H4 Histone as a Read-out for Neutrophil Extracellular Traps Formation
    一种成像流式细胞术测定组蛋白H4瓜氨酸化的方法,作为中性粒细胞胞外陷阱形成的信号通路
    作者:Emilia A. Barbu, Venina M. Dominical, Laurel Mendelsohn and Swee Lay Thein日期:02/20/2021,浏览量:1531,Q&A: 0
    [Abstract]

    The formation of neutrophil extracellular traps (NETs) is thought to play a critical role in infections and propagating sterile inflammation. Histone citrullination is an essential and early step in NETs formation, detectable prior to the formation of the hallmark extracellular DNA-scaffolded strands. In addition to the classical microscopy

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    Fibroblast Gap-closure Assay-Microscopy-based in vitro Assay Measuring the Migration of Murine Fibroblasts
    基于成纤维细胞间隙封闭显微成像的鼠成纤维细胞的体外迁移分析
    作者:Agnieszka P. Looney and Mallar Bhattacharya日期:08/20/2019,浏览量:3066,Q&A: 0
    [Abstract] Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts.

    Currently, the two most widely used chemotaxis and cell migration assays are the scratch assay and the ...
    Early Life Ovalbumin Sensitization and Aerosol Challenge for the Induction of Allergic Airway Inflammation in a BALB/c Murine Model
    BALB/c小鼠模型中早期卵白蛋白致敏和气溶胶攻击诱导的过敏性气道炎症
    [Abstract] The early life period represents a time of immunological plasticity whereby the functionally immature immune system is highly susceptible to environmental stimulation. Perennial aeroallergen and respiratory viral infection induced sporadic episodes of lung inflammation during this temporal window represent major risk factors for initiation of ...
    Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation
    通过氯化锂沉淀法纯化DSS处理的鼠组织的总RNA
    作者:Emilie Viennois, Anika Tahsin and Didier Merlin日期:05/05/2018,浏览量:5578,Q&A: 0
    [Abstract] We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium chloride.
    Mono Sodium Urate Crystal-induced Peritonitis for in vivo Assessment of Inflammasome Activation
    单钠尿酸盐晶体诱导的腹膜炎用于体内评估炎症体激活
    作者:Marianne R. Spalinger and Michael Scharl日期:03/05/2018,浏览量:6594,Q&A: 0
    [Abstract] Due to its particulate material, mono-sodium urate (MSU) crystals are potent activators of the NOD-like receptor NLRP3. Upon activation, NLRP3 induces the formation of inflammasome complexes, which lead to the production and release of mature IL-1β. Bioactive IL-1β is a potent activator of innate immune responses and promotes recruitment of ...
    EAE Induction by Passive Transfer of MOG-specific CD4+ T Cells
    通过MOG特异性CD4+ T细胞的被动转移诱导EAE
    [Abstract] Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS), which is a chronic inflammatory disease of the central nervous system (CNS). It is characterized by focal demyelination and inflammatory responses mediated by myelin-specific autoreactive CD4+ T cells. Using a passive transfer model of EAE in ...
    Detection of ASC Oligomerization by Western Blotting
    蛋白印迹法检测ASC寡聚化
    作者:Jérôme Lugrin and Fabio Martinon 日期:05/20/2017,浏览量:14281,Q&A: 0
    [Abstract] The apoptosis-associated speck-like protein with a caspase-recruitment domain (ASC) adaptor protein bridges inflammasome sensors and caspase-1. Upon inflammasome activation, ASC nucleates in a prion-like manner into a large and single platform responsible for the recruitment and the activation of caspase-1. Active caspase-1 will in turn promote ...
    Collagen-induced Arthritis: A Model for Murine Autoimmune Arthritis
    胶原蛋白诱导的关节炎:构建鼠自身免疫性关节炎的模型
    作者:Kathryn M. Pietrosimone, Mengyao Jin, Brad Poston and Peng Liu日期:10/20/2015,浏览量:11459,Q&A: 1
    [Abstract] Collagen-induced arthritis (CIA) is a common autoimmune animal model used to study rheumatoid arthritis (RA). The development of CIA involves infiltration of macrophages and neutrophils into the joint, as well as T and B cell responses to type II collagen. In murine CIA, genetically susceptible mice (DBA/1J) are immunized with a type II bovine ...