生物化学

分类

    现刊
    Reconstitution of Chromatin by Stepwise Salt Dialysis
    逐步盐透析重组染色质
    作者:Grisel Cruz-Becerra and James T. Kadonaga日期:04/05/2021,浏览量:1630,Q&A: 0
    [Abstract]

    Chromatin, rather than plain DNA, is the natural substrate of the molecular machines that mediate DNA-directed processes in the nucleus. Chromatin can be reconstituted in vitro by using different methodologies. The salt dialysis method yields chromatin that consists of purified histones and DNA. This biochemically pure chromatin is well-suited for

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    Assembly of Genetic Circuits with the Mammalian ToolKit
    用哺乳动物工具包组装遗传电路
    作者:João P. Fonseca, Alain R Bonny, Jason Town and Hana El-Samad日期:03/05/2020,浏览量:2843,Q&A: 0
    [Abstract] The ability to rapidly assemble and prototype cellular circuits is vital for biological research and its applications in biotechnology and medicine. The Mammalian ToolKit (MTK) is a Golden Gate-based cloning toolkit for fast, reproducible and versatile assembly of large DNA vectors and their implementation in mammalian models. The MTK consists of ...
    Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120
    反义RNA介导的鱼腥藻PCC 7120基因敲除株的构建
    作者:Amit Srivastava, Anand Ballal, Karl Forchhammer and Anil Kumar Tripathi日期:02/20/2020,浏览量:2820,Q&A: 0
    [Abstract] Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many genes in Anabaena encode an essential life function and hence cannot be deleted, causing a ...
    Evaluation of Genotoxicity by Micronucleus Assay in vitro and by Allium cepa Test in vivo
    利用微核体外分析系统和洋葱体内实验进行遗传毒性测试
    作者:Christina N. Banti and Sotiris K. Hadjikakou日期:07/20/2019,浏览量:5307,Q&A: 0
    [Abstract] The in vitro and in vivo genotoxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) is evaluated by the micronucleus test and the Allium cepa assay, respectively. Fetal lung fibroblast cells (MRC-5), normal human corneal epithelial cells (HCEC) and ...
    DNA Fiber Assay upon Treatment with Ultraviolet Radiations
    紫外线辐射处理后DNA纤维的检测
    作者:Alfano Luigi, Antonio Giordano and Francesca Pentimalli日期:06/05/2017,浏览量:15054,Q&A: 1
    [Abstract] Genome stability is continuously challenged by a wide range of DNA damaging factors. To promote a correct DNA repair and cell survival, cells orchestrate a coordinated and finely tuned cascade of events collectively known as the DNA Damage Response (DDR). Ultra Violet (UV) rays are among the main environmental sources of DNA damage and a well ...
    Single-molecule Analysis of DNA Replication Dynamics in Budding Yeast and Human Cells by DNA Combing
    采用DNA梳理技术对芽殖酵母和人类细胞中DNA复制动力学进行单分子分析
    作者:Hélène Tourrière, Julie Saksouk, Armelle Lengronne and Philippe Pasero 日期:06/05/2017,浏览量:10972,Q&A: 0
    [Abstract] The DNA combing method allows the analysis of DNA replication at the level of individual DNA molecules stretched along silane-coated glass coverslips. Before DNA extraction, ongoing DNA synthesis is labeled with halogenated analogues of thymidine. Replication tracks are visualized by immunofluorescence using specific antibodies. Unlike biochemical ...
    Cloud-point PEG Glass Surfaces for Imaging of Immobilized Single Molecules by Total-internal-reflection Microscopy
    采用浊点PEG玻璃表面法和全内反射显微镜进行固定化单分子成像
    作者:Zhengjian Zhang, Sang Ryul Park, Alexandros Pertsinidis and Andrey Revyakin日期:04/05/2016,浏览量:9413,Q&A: 0
    [Abstract] This effective, robust protocol generates glass coverslips coated with biotin-functionalized polyethylene glycol (PEG), making the glass surface resistant to non-specific absorption of biomolecules, and permitting immobilization of biomolecules for subsequent single-molecule tracking of biochemical reactions. The protocol can be completed in one ...