癌症生物学

分类

    现刊
    Modeling the Nonlinear Dynamics of Intracellular Signaling Networks
    细胞内信号网络的非线性动力学建模
    作者:Oleksii S. Rukhlenko and Boris N. Kholodenko日期:07/20/2021,浏览量:1183,Q&A: 0
    [Abstract]

    This protocol illustrates a pipeline for modeling the nonlinear behavior of intracellular signaling pathways. At fixed spatial points, nonlinear signaling dynamics are described by ordinary differential equations (ODEs). At constant parameters, these ODEs may have multiple attractors, such as multiple steady states or limit cycles. Standard

    ...
    Atomic Force Microscopy to Characterize Ginger Lipid-Derived Nanoparticles (GLDNP)
    原子力显微镜研究生姜脂质纳米颗粒(GLDNP)
    作者:Dingpei Long, Chunhua Yang, Junsik Sung and Didier Merlin日期:04/05/2021,浏览量:2776,Q&A: 0
    [Abstract]

    We have demonstrated that a specific population of ginger-derived nanoparticles (GDNP-2) could effectively target the colon, reduce colitis, and alleviate colitis-associated colon cancer. Naturally occurring GDNP-2 contains complex bioactive components, including lipids, proteins, miRNAs, and ginger secondary metabolites (gingerols and shogaols).

    ...
    Intestinal Co-culture System to Study TGR5 Agonism and Gut Restriction
    利用肠道共培养系统研究TGR5激动剂和肠道限制性
    作者:Snehal N. Chaudhari and A. Sloan Devlin日期:03/20/2021,浏览量:2259,Q&A: 1
    [Abstract]

    The activation of the Takeda G-protein receptor 5 (TGR5, also known as the G protein-coupled bile acid receptor 1, GPBAR1) in enteroendocrine L-cells results in secretion of the anti-diabetic hormone Glucagon-Like Peptide 1 (GLP-1) into systemic circulation. Consequently, recent research has focused on identification and development of TGR5

    ...
    RI-SEC-seq: Comprehensive Profiling of Nonvesicular Extracellular RNAs with Different Stabilities
    RI-SEC-seq:具有不同稳定性的非泡性细胞外RNA的综合分析
    作者:Juan Pablo Tosar, Fabiana Gámbaro, Mauricio Castellano and Alfonso Cayota日期:02/20/2021,浏览量:2402,Q&A: 0
    [Abstract]

    Exosomes and other extracellular vesicles (EVs) are considered the main vehicles transporting RNAs in extracellular samples, including human bodily fluids. However, a major proportion of extracellular RNAs (exRNAs) do not copurify with EVs and remain in ultracentrifugation supernatants of cell-conditioned medium or blood serum. We have observed

    ...
    A mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases
    用Mant-GDP分离实验比较小GTP蛋白的鸟嘌呤核苷酸的结合偏好
    作者:Yuping Tan and Qingxiang Sun日期:01/20/2021,浏览量:2142,Q&A: 0
    [Abstract]

    Small GTPases are cellular switches that are switched on when bound to GTP and switched off when bound to GDP. Different small GTPase proteins or those with mutations may bind to GTP or GDP with different relative affinities. However, small GTPases generally have very high affinities for guanine nucleotides, rendering it difficult to compare the

    ...
    Evaluation of Toxicity with Brine Shrimp Assay
    通过卤虫实验进行毒性评价
    作者:Christina N. Banti and Sotiris K. Hadjikakou日期:01/20/2021,浏览量:2551,Q&A: 0
    [Abstract]

    The in vivo toxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) or their hydrogels such as with hydroxyethyl-methacrylate (HEMA) (pHEMA@SBAMs or pHEMA@CoMeDs) are evaluated by the brine shrimp assay. Thus individuals of Artemia salina larvae are incubated in saline solutions with

    ...
    Real-time Three-dimensional Tracking of Endocytic Vesicles
    内囊泡的实时三维追踪
    作者:Stephanie Duhamel and Kossay Zaoui日期:10/20/2020,浏览量:1590,Q&A: 0
    [Abstract] Endocytic trafficking and recycling are fundamental cellular processes that control essential functions such as signaling protein complexes transport and membrane identity. The small GTPase Rabs are indispensable component of the endosomal recycling machinery. The Rabs bind to effectors to mediate their functions, such as protein sorting and ...
    Quantification of Salivary Charged Metabolites Using Capillary Electrophoresis Time-of-flight-mass Spectrometry
    唾液中带电荷代谢产物的毛细管电泳与飞行时间质谱联用定量分析
    作者:Masahiro Sugimoto, Sana Ota, Miku Kaneko, Ayame Enomoto and Tomoyoshi Soga日期:10/20/2020,浏览量:1442,Q&A: 0
    [Abstract] Salivary metabolomics have provided the potentials to detect both oral and systemic diseases. Capillary electrophoresis time-of-flight-mass spectrometry (CE-TOFMS) enables the identification and quantification of various charged metabolites. This method has been employed to biomarker discoveries using human saliva samples, especially for various ...
    Tyramide Signal-Amplified Immunofluorescence of MYCN and MYC in Human Tissue Specimens and Cell Line Cultures
    酪胺信号放大的人体组织标本和细胞系培养物中的MYCN和MYC免疫荧光
    作者:Johanna M. Schafer and Jennifer A. Pietenpol日期:07/05/2020,浏览量:1760,Q&A: 0
    [Abstract] MYC family members, MYC, MYCN, and MYCL, are oncogenic transcription factors that regulate the expression of genes involved in normal development, cell growth, proliferation, metabolism, and survival. While MYC is amplified and/or overexpressed across a variety of tissue types, MYCN is often overexpressed in tumors of the nervous system ...
    Colorimetric RhoB GTPase Activity Assay
    比色法测定RhoB-GTPase活性
    作者:Kossay Zaoui and Stephanie Duhamel日期:05/05/2020,浏览量:2572,Q&A: 0
    [Abstract] The Ras homologous protein (Rho) GTPase subfamily, including RhoA, RhoB, and RhoC are small molecules (~21 kDa) that act as molecular switches in a wide range of signaling pathways to orchestrate biological processes associated with both physiological and tumorigenic cellular states. The Rho GTPases are crucial regulators of actin cytoskeleton ...