生物物理学

分类

    现刊
    Using Atomic Force Microscopy to Study the Real Time Dynamics of DNA Unwinding by Mitochondrial Twinkle Helicase
    使用原子力显微镜研究线粒体闪烁解旋酶对DNA解旋的实时动力学
    作者:Parminder Kaur, Hai Pan, Matthew J. Longley, William C. Copeland and Hong Wang日期:09/05/2021,浏览量:723,Q&A: 0
    [Abstract]

    Understanding the structure and dynamics of DNA-protein interactions during DNA replication is crucial for elucidating the origins of disorders arising from its dysfunction. In this study, we employed Atomic Force Microscopy as a single-molecule imaging tool to examine the mitochondrial DNA helicase Twinkle and its interactions with DNA. We used

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    Unraveling the Physicochemical Determinants of Protein Liquid-liquid Phase Separation by Nanoscale Infrared Vibrational Spectroscopy
    利用纳米尺度红外振动光谱揭示蛋白质液-液相分离的理化决定因素
    作者:Francesco S. Ruggeri, Alyssa M. Miller, Michele Vendruscolo and Tuomas P. J. Knowles日期:08/20/2021,浏览量:861,Q&A: 0
    [Abstract]

    The phenomenon of reversible liquid-liquid phase separation of proteins underlies the formation of membraneless organelles, which are crucial for cellular processes such as signalling and transport. In addition, it is also of great interest to uncover the mechanisms of further irreversible maturation of the functional dense liquid phase into

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    Purification of Mitochondrial Ribosomes with the Translocase Oxa1L from HEK Cells
    用转位酶Oxa1L纯化HEK细胞线粒体核糖体
    作者:Hanting Yang and Nirupa Desai日期:08/05/2021,浏览量:967,Q&A: 0
    [Abstract]

    Mitochondrial ribosomes (mitoribosomes) perform protein synthesis inside mitochondria, the organelles responsible for energy conversion and adenosine triphosphate (ATP) production in eukaryotic cells. To investigate their functions and structures, large-scale purification of intact mitoribosomes from mitochondria-rich animal tissues or HEK cells

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    Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
    植物线粒体核糖体的纯化和低温电镜分析
    作者:Florent Waltz, Philippe Giegé and Yaser Hashem日期:08/05/2021,浏览量:767,Q&A: 0
    [Abstract]

    Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production. Among mitochondrial gene expression processes, translation is the least understood; although, recent advances

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    Spatiotemporal Quantification of Cytosolic pH in Arabidopsis Pollen Tubes
    拟南芥花粉管胞质pH的时空定量研究
    作者:Maria Teresa Portes, Daniel S.C. Damineli and José A. Feijó日期:07/20/2021,浏览量:809,Q&A: 0
    [Abstract]

    Ion-specific probes and fluorescent indicators have been key in establishing the role of ion signaling, namely calcium, protons, and anions, in plant development, providing a robust approach for monitoring spatiotemporal changes in intracellular ion dynamics. The integration of protons/pH in signaling mechanisms is especially important as reports

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    A Multi-color Bicistronic Biosensor to Compare the Translation Dynamics of Different Open Reading Frames at Single-molecule Resolution in Live Cells
    一种多色双顺反子生物传感器比较活细胞中不同开放阅读框在单分子分辨率下的翻译动力学
    作者:Amanda L. Koch, Tatsuya Morisaki and Timothy J. Stasevich日期:07/20/2021,浏览量:1127,Q&A: 0
    [Abstract]

    Here, we describe how to image and quantitate the translation dynamics of a bicistronic biosensor that we recently created to fairly compare cap-dependent and IRES-mediated translation at single-molecule resolution in live human cells. This technique employs a pair of complementary intrabodies loaded into living cells that co-translationally bind

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    Single-molecule Fluorescence Technique to Monitor the Co-transcriptional Formation of G-quadruplex and R-loop Structures
    单分子荧光技术监测G-四链体和R-环结构的共转录形成
    作者:Gunhyoung Lim and Sungchul Hohng日期:07/05/2021,浏览量:1625,Q&A: 0
    [Abstract]

    G-quadruplexes (GQ) and R-loops are non-canonical nucleic acid structures related to gene regulation and genome instability that can be formed during transcription; however, their formation mechanisms remain elusive. To address this question, we developed a single-molecule fluorescence technique to monitor the formation of G-quadruplex and R-loop

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    Building a Total Internal Reflection Microscope (TIRF) with Active Stabilization (Feedback SMLM)
    建立一个主动稳定的全内反射显微镜(TIRF)
    作者:Simao Coelho, Jongho Baek, J. Justin Gooding and Katharina Gaus日期:07/05/2021,浏览量:1818,Q&A: 0
    [Abstract]

    The data quality of high-resolution imaging can be markedly improved with active stabilization, which is based on feedback loops within the microscope that maintain the sample in the same location throughout the experiment. The purpose is to provide a highly accurate focus lock, therefore eliminating drift and improving localization precision.

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    Electron Tomography to Study the Three-dimensional Structure of the Reovirus Egress Pathway in Mammalian Cells
    电子断层扫描研究哺乳动物细胞呼肠孤病毒出口途径的三维结构
    作者:Isabel Fernández de Castro, Jose Jesús Fernández, Terence S. Dermody and Cristina Risco日期:07/05/2021,浏览量:1397,Q&A: 0
    [Abstract]

    Mammalian orthoreoviruses (reoviruses) are nonenveloped, double-stranded RNA viruses that replicate and assemble in cytoplasmic membranous organelles called viral inclusions (VIs). To define the cellular compartments involved in nonlytic reovirus egress, we imaged viral egress in infected, nonpolarized human brain microvascular endothelial cells

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    Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy
    单粒子低温电子显微镜双态微管馏分的制备
    作者:Corbin Black, Daniel Chen Dai, Katya Peri, Muneyoshi Ichikawa and Khanh Huy Bui日期:06/05/2021,浏览量:1745,Q&A: 0
    [Abstract]

    Over the years, studying the ultrastructure of the eukaryotic cilia/flagella using electron microscopy (EM) has contributed significantly toward our understanding of ciliary function. Major complexes in the cilia, such as inner and outer dynein arms, radial spokes, and dynein regulatory complexes, were originally discovered by EM. Classical

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